The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Su, S. B.
Right arrow Articles by Caspi, R. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Su, S. B.
Right arrow Articles by Caspi, R. R.
Right arrowPubmed/NCBI databases
*Substance via MeSH
Medline Plus Health Information
*Autoimmune Diseases
The Journal of Immunology, 2001, 167: 250-256.
Copyright © 2001 by The American Association of Immunologists

Pertussis Toxin Inhibits Induction of Tissue-Specific Autoimmune Disease by Disrupting G Protein-Coupled Signals

Shao Bo Su, Phyllis B. Silver, Meifen Zhang, Chi-Chao Chan and Rachel R. Caspi1

Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892

Pertussis toxin (PTX) has been used for many years as an adjuvant that promotes development of tissue-specific experimental autoimmune diseases such as experimental autoimmune encephalomyelitis, experimental autoimmune uveitis (EAU), and others. Enhancement of vascular permeability and of Th1 responses have been implicated in this effect. Here we report a surprising observation that, in a primed system, PTX can completely block the development of EAU. Disease was induced in B10.RIII mice by adoptive transfer of uveitogenic T cells, or by immunization with a uveitogenic peptide. A single injection of PTX concurrently with infusion of the uveitogenic T cells, or two injections 7 and 10 days after active immunization, completely blocked development of EAU. EAU also was prevented by a 1-h incubation in vitro of the uveitogenic T cells with PTX before infusing them into recipients. Uveitogenic T cells treated with PTX in vitro and lymphoid cells from mice treated with PTX in vivo failed to migrate to chemokines in a standard chemotaxis assay. Neither the isolated B-oligomer subunit of PTX that lacks ADP ribosyltransferase activity nor the related cholera toxin that ADP-ribosylates Gs (but not Gi) proteins blocked EAU induction or migration to chemokines. We conclude that PTX present at the time of cell migration to the target organ prevents EAU, and propose that it does so at least in part by disrupting signaling through Gi protein-coupled receptors. Thus, the net effect of PTX on autoimmune disease would represent an integration of enhancing and inhibitory effects.




This article has been cited by other articles:


Home page
 IOVSHome page
B. J. E. Raveney, C. Richards, M.-L. Aknin, D. A. Copland, B. R. Burton, E. Kerr, L. B. Nicholson, N. A. Williams, and A. D. Dick
The B Subunit of Escherichia coli Heat-Labile Enterotoxin Inhibits Th1 but Not Th17 Cell Responses in Established Experimental Autoimmune Uveoretinitis
Invest. Ophthalmol. Vis. Sci., September 1, 2008; 49(9): 4008 - 4017.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
S. B. Su, R. S. Grajewski, D. Luger, R. K. Agarwal, P. B. Silver, J. Tang, J. Tuo, C.-C. Chan, and R. R. Caspi
Altered Chemokine Profile Associated with Exacerbated Autoimmune Pathology under Conditions of Genetic Interferon-{gamma} Deficiency
Invest. Ophthalmol. Vis. Sci., October 1, 2007; 48(10): 4616 - 4625.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
U. M. Padigel, L. Stein, K. Redding, J. J. Lee, T. J. Nolan, G. A. Schad, L. Birnbaumer, and D. Abraham
Signaling through G{alpha}i2 protein is required for recruitment of neutrophils for antibody-mediated elimination of larval Strongyloides stercoralis in mice
J. Leukoc. Biol., April 1, 2007; 81(4): 1120 - 1126.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. Cassan, E. Piaggio, J. P. Zappulla, L. T. Mars, N. Couturier, F. Bucciarelli, S. Desbois, J. Bauer, D. Gonzalez-Dunia, and R. S. Liblau
Pertussis Toxin Reduces the Number of Splenic Foxp3+ Regulatory T Cells
J. Immunol., August 1, 2006; 177(3): 1552 - 1560.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Bo Su, P. B. Silver, P. Wang, C.-C. Chan, and R. R. Caspi
Cholera Toxin Prevents Th1-Mediated Autoimmune Disease by Inducing Immune Deviation
J. Immunol., July 15, 2004; 173(2): 755 - 761.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. B. Su, P. B. Silver, P. Wang, C.-C. Chan, and R. R. Caspi
Dissociating the Enhancing and Inhibitory Effects of Pertussis Toxin on Autoimmune Disease
J. Immunol., September 1, 2003; 171(5): 2314 - 2319.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. H. Hofstetter, C. L. Shive, and T. G. Forsthuber
Pertussis Toxin Modulates the Immune Response to Neuroantigens Injected in Incomplete Freund's Adjuvant: Induction of Th1 Cells and Experimental Autoimmune Encephalomyelitis in the Presence of High Frequencies of Th2 Cells
J. Immunol., July 1, 2002; 169(1): 117 - 125.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. F. Foxman, M. Zhang, S. D. Hurst, T. Muchamuel, D. Shen, E. F. Wawrousek, C.-C. Chan, and I. Gery
Inflammatory Mediators in Uveitis: Differential Induction of Cytokines and Chemokines in Th1- Versus Th2-Mediated Ocular Inflammation
J. Immunol., March 1, 2002; 168(5): 2483 - 2492.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2001 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2001 by The American Association of Immunologists, Inc. All rights reserved.