| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Medicine, University of Wales, College of Medicine, Cardiff, United Kingdom
Dendritic cells (DCs) play a pivotal role in the development of
anti-viral CD8+ CTL responses. This is
straightforward if they are directly infected with virus, but is less
clear in response to viruses that cannot productively infect DCs. Human
CMV (HCMV) shows strain-specific cell tropism: fibroblast (Fb)-adapted
laboratory strains (AD169) and recent clinical isolates do not infect
DCs, whereas endothelial cell-adapted strains (TB40/E) result in
productive lytic DC infection. However, we show here that uninfected
DCs induce CD8+ T cell cytotoxicity and IFN-
production
against HCMV pp65 and immediate early 1 Ags following in vitro
coculture with HCMV-AD169-infected Fbs, regardless of the HLA type of
these Fbs. CD8+ T cell stimulation was inhibited by
pretreatment of DCs with cytochalasin B or brefeldin A, indicating a
phagosome/endosome to cytosol pathway. HCMV-infected Fbs were not
apoptotic as measured by annexin V binding, and induction of apoptosis
of infected Fbs in vitro did not augment CTL induction by DCs,
suggesting a mechanism other than apoptosis in the initiation of
cross-presentation. Furthermore, HCMV-infected Fbs provided a
maturation signal for immature DCs during coculture, as evidenced by
increased CD83 and HLA class II expression. Cross-presentation of HCMV
Ags by host DCs enables these professional APCs to bypass some of the
evasion mechanisms HCMV has developed to avoid T cell recognition. It
may also serve to explain the presence of immediate early 1 Ag-specific
CTLs in the face of pp65-induced inhibition of Ag presentation at the
level of the infected cell.
This article has been cited by other articles:
|
|
 |
|
  M. M. Weck, S. Appel, D. Werth, C. Sinzger, A. Bringmann, F. Grunebach, and P. Brossart hDectin-1 is involved in uptake and cross-presentation of cellular antigens Blood, April 15, 2008; 111(8): 4264 - 4272. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Weck, F. Grunebach, D. Werth, C. Sinzger, A. Bringmann, and P. Brossart TLR ligands differentially affect uptake and presentation of cellular antigens Blood, May 1, 2007; 109(9): 3890 - 3894. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. N. Posnett, M. E. Engelhorn, and A. N. Houghton Antiviral T cell responses: phalanx or multipronged attack? J. Exp. Med., June 20, 2005; 201(12): 1881 - 1884. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Bosnjak, M. Miranda-Saksena, D. M. Koelle, R. A. Boadle, C. A. Jones, and A. L. Cunningham Herpes Simplex Virus Infection of Human Dendritic Cells Induces Apoptosis and Allows Cross-Presentation via Uninfected Dendritic Cells J. Immunol., February 15, 2005; 174(4): 2220 - 2227. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Manley, L. Luy, T. Jones, M. Boeckh, H. Mutimer, and S. R. Riddell Immune evasion proteins of human cytomegalovirus do not prevent a diverse CD8+ cytotoxic T-cell response in natural infection Blood, August 15, 2004; 104(4): 1075 - 1082. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. N. Fleeton, N. Contractor, F. Leon, J. D. Wetzel, T. S. Dermody, and B. L. Kelsall Peyer's Patch Dendritic Cells Process Viral Antigen from Apoptotic Epithelial Cells in the Intestine of Reovirus-infected Mice J. Exp. Med., July 19, 2004; 200(2): 235 - 245. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Li Pira, L. Bottone, F. Ivaldi, R. Pelizzoli, F. Del Galdo, L. Lozzi, L. Bracci, A. Loregian, G. Palu, R. De Palma, et al. Identification of new Th peptides from the cytomegalovirus protein pp65 to design a peptide library for generation of CD4 T cell lines for cellular immunoreconstitution Int. Immunol., May 1, 2004; 16(5): 635 - 642. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Moutaftsi, P. Brennan, S. A. Spector, and Z. Tabi Impaired Lymphoid Chemokine-Mediated Migration due to a Block on the Chemokine Receptor Switch in Human Cytomegalovirus-Infected Dendritic Cells J. Virol., March 15, 2004; 78(6): 3046 - 3054. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Khan, A. Zimmermann, M. Basler, M. Groettrup, and H. Hengel A Cytomegalovirus Inhibitor of Gamma Interferon Signaling Controls Immunoproteasome Induction J. Virol., February 15, 2004; 78(4): 1831 - 1842. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. Fonteneau, D. G. Kavanagh, M. Lirvall, C. Sanders, T. L. Cover, N. Bhardwaj, and M. Larsson Characterization of the MHC class I cross-presentation pathway for cell-associated antigens by human dendritic cells Blood, December 15, 2003; 102(13): 4448 - 4455. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Hertel, V. G. Lacaille, H. Strobl, E. D. Mellins, and E. S. Mocarski Susceptibility of Immature and Mature Langerhans Cell-Type Dendritic Cells to Infection and Immunomodulation by Human Cytomegalovirus J. Virol., July 1, 2003; 77(13): 7563 - 7574. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Karrer, S. Sierro, M. Wagner, A. Oxenius, H. Hengel, U. H. Koszinowski, R. E. Phillips, and P. Klenerman Memory Inflation: Continuous Accumulation of Antiviral CD8+ T Cells Over Time J. Immunol., February 15, 2003; 170(4): 2022 - 2029. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Koelle and L. Corey Recent Progress in Herpes Simplex Virus Immunobiology and Vaccine Research Clin. Microbiol. Rev., January 1, 2003; 16(1): 96 - 113. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Kondo, M. S. Topp, H.-P. Kiem, Y. Obata, Y. Morishima, K. Kuzushima, M. Tanimoto, M. Harada, T. Takahashi, and Y. Akatsuka Efficient Generation of Antigen-Specific Cytotoxic T Cells Using Retrovirally Transduced CD40-Activated B Cells J. Immunol., August 15, 2002; 169(4): 2164 - 2171. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Moutaftsi, A. M. Mehl, L. K. Borysiewicz, and Z. Tabi Human cytomegalovirus inhibits maturation and impairs function of monocyte-derived dendritic cells Blood, April 15, 2002; 99(8): 2913 - 2921. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-Q. Zhao, X.-L. Huang, P. Gupta, L. Borowski, Z. Fan, S. C. Watkins, E. K. Thomas, and C. R. Rinaldo Jr. Induction of Anti-Human Immunodeficiency Virus Type 1 (HIV-1) CD8+ and CD4+ T-Cell Reactivity by Dendritic Cells Loaded with HIV-1 X4-Infected Apoptotic Cells J. Virol., February 22, 2002; 76(6): 3007 - 3014. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Arrode, C. Boccaccio, J.-P. Abastado, and C. Davrinche Cross-Presentation of Human Cytomegalovirus pp65 (UL83) to CD8+ T Cells Is Regulated by Virus-Induced, Soluble-Mediator-Dependent Maturation of Dendritic Cells J. Virol., January 1, 2002; 76(1): 142 - 150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Kammerer, D. Stober, P. Riedl, C. Oehninger, R. Schirmbeck, and J. Reimann Noncovalent Association with Stress Protein Facilitates Cross-Priming of CD8+ T Cells to Tumor Cell Antigens by Dendritic Cells J. Immunol., January 1, 2002; 168(1): 108 - 117. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
