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Department of Pathology and Comprehensive Kaplan Cancer Center, New York University School of Medicine, New York, NY 10016; and
Department of Pathology and Laboratory Medicine, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark, NJ 07103
Spontaneous germinal center (GC)-derived B cell lymphomas of
SJL mice (RCS) transcribe a 1.8-kb Mtv-29 mRNA under
control of the META-env promoter. The encoded vSAg29
stimulates syngeneic V
16+ CD4+ T cells,
thereby acquiring T cell help necessary for RCS growth. Other strains
of B cell lymphoma-prone mice include Mtv29+
C57L and MA/MyJ, and the Mtv29-
Mtv7+-recombinant inbred strain, SW x
J-1. The lymphomas of these mice produce similar mouse
mtv-vSAg-encoding mRNA, as characterized by Northern blotting, PCR, and
RNase protection. A 1.8-kb mRNA in C57L/J and MA/MyJ lymphomas
hybridized with an Mtv29-specific oligonucleotide,
whereas SW x J-1 lymphomas produced 1.8-kb transcripts
hybridizing with an Mtv7-specific oligonucleotide.
Similar META-env-initiated transcripts were absent from LPS-activated B
cells from any strain examined but were detected in Peyer’s patch RNA
from SJL mice. Like typical SJL-derived RCS, all these lymphomas
stimulated syngeneic CD4+ T cells and V16+ T
hybridoma cells. Immunohistochemical staining of primary tumors showed
the presence of peanut agglutinin binding (PNA+) highly
mitotic lymphoblasts, suggesting their GC derivation. The findings
indicate that this novel mRNA for Mtv29 is present in B
cell lymphomas from several Mtv29+ mouse
strains. Additionally, this is the first description of the ability of
Mtv7 to produce transcripts that are controlled and
spliced identically to those of Mtv29 and that are
expressed in SW x J-1, I-As+, lymphomas that also
stimulate V16+ T cells. Our results suggest an important
role for mouse mtv-vSAgs and V16 T cell stimulation in the
development of GC-derived murine B cell
lymphomas.
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