HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fantuzzi, L. Articles by Gessani, S. Search for Related Content PubMed PubMed Citation Articles by Fantuzzi, L. Articles by Gessani, S.

HIV-1 gp120 Stimulates the Production of -Chemokines in Human Peripheral Blood Monocytes Through a CD4-Independent Mechanism¹

Laboratory of Virology, Istituto Superiore di Sanità, Rome, Italy

The present study was designed to evaluate the effect of the HIV-1 envelope glycoprotein gp120 on the expression of -chemokines in cultured monocytes/macrophages. Treatment of either freshly isolated 1-day-cultured monocytes or 7-day-cultured monocyte-derived macrophages (MDM) with recombinant gp120-IIIB resulted in a specific and dose-dependent enhancement of secretion of monocyte chemoattractant protein-1, macrophage inflammatory protein-1, and RANTES as well as a clear-cut increase in transcript accumulation. The expression of these mRNA was increased, but not superinduced, in the presence of cycloheximide. -Chemokine secretion was also induced after exposure of monocyte cultures to gp120-JRFL and aldrithiol-2-inactivated R5 and X4 HIV-1 strains, retaining conformational and functional integrity of envelope proteins. In contrast, no -chemokine secretion was triggered by X4 and R5 gp120 or aldrithiol-2-inactivated virus treatment of monocytoid cell lines that were fully responsive to LPS. The gp120-mediated effect was independent of its interaction with CD4, as preincubation with soluble CD4 did not abrogate -chemokine induction. Moreover, triggering of CD4 receptor by a specific Ab did not result in any -chemokine secretion. Interestingly, engagement of CCR5 and CXCR4 receptors by specific Abs as well as treatment with CCR5 and CXCR4 ligands induced -chemokine secretion. On the whole, these results indicate that HIV-1 stimulates monocytes/macrophages to produce -chemokines by a specific interaction of gp120 with HIV-1 coreceptors on the cell membrane. The expression of these related polypeptides may represent an important cellular response for regulating both the extent of viral infection and the recruitment of immune cells.

This article has been cited by other articles:

				M. Del Corno, A. Michienzi, A. Masotti, L. Da Sacco, G. F. Bottazzo, F. Belardelli, and S. Gessani CC chemokine ligand 2 down-modulation by selected Toll-like receptor agonist combinations contributes to T helper 1 polarization in human dendritic cells Blood, July 23, 2009; 114(4): 796 - 806. [Abstract] [Full Text] [PDF]

				M. S. Giri, M. Nebozyhn, A. Raymond, B. Gekonge, A. Hancock, S. Creer, C. Nicols, M. Yousef, A. S. Foulkes, K. Mounzer, et al. Circulating Monocytes in HIV-1-Infected Viremic Subjects Exhibit an Antiapoptosis Gene Signature and Virus- and Host-Mediated Apoptosis Resistance J. Immunol., April 1, 2009; 182(7): 4459 - 4470. [Abstract] [Full Text] [PDF]

				G. R. Campbell and S. A. Spector CCL2 Increases X4-tropic HIV-1 Entry into Resting CD4+ T Cells J. Biol. Chem., November 7, 2008; 283(45): 30745 - 30753. [Abstract] [Full Text] [PDF]

				L. Fantuzzi, F. Spadaro, C. Purificato, S. Cecchetti, F. Podo, F. Belardelli, S. Gessani, and C. Ramoni Phosphatidylcholine-specific phospholipase C activation is required for CCR5-dependent, NF-kB-driven CCL2 secretion elicited in response to HIV-1 gp120 in human primary macrophages Blood, April 1, 2008; 111(7): 3355 - 3363. [Abstract] [Full Text] [PDF]

				H. J. J. van der Vliet, M. G. A. van Vonderen, J. W. Molling, H. J. Bontkes, M. Reijm, P. Reiss, M. A. van Agtmael, S. A. Danner, A. J. M. van den Eertwegh, B. M. E. von Blomberg, et al. Cutting Edge: Rapid Recovery of NKT Cells upon Institution of Highly Active Antiretroviral Therapy for HIV-1 Infection J. Immunol., November 1, 2006; 177(9): 5775 - 5778. [Abstract] [Full Text] [PDF]

				B. Tomkowicz, C. Lee, V. Ravyn, R. Cheung, A. Ptasznik, and R. G. Collman The Src kinase Lyn is required for CCR5 signaling in response to MIP-1beta and R5 HIV-1 gp120 in human macrophages Blood, August 15, 2006; 108(4): 1145 - 1150. [Abstract] [Full Text] [PDF]

				P. Ancuta, P. Autissier, A. Wurcel, T. Zaman, D. Stone, and D. Gabuzda CD16+ Monocyte-Derived Macrophages Activate Resting T Cells for HIV Infection by Producing CCR3 and CCR4 Ligands J. Immunol., May 15, 2006; 176(10): 5760 - 5771. [Abstract] [Full Text] [PDF]

				M. Del Corno, M. C. Gauzzi, G. Penna, F. Belardelli, L. Adorini, and S. Gessani Human Immunodeficiency Virus Type 1 gp120 and Other Activation Stimuli Are Highly Effective in Triggering Alpha Interferon and CC Chemokine Production in Circulating Plasmacytoid but Not Myeloid Dendritic Cells J. Virol., October 1, 2005; 79(19): 12597 - 12601. [Abstract] [Full Text] [PDF]

				C. Lee, B. Tomkowicz, B. D. Freedman, and R. G. Collman HIV-1 gp120-induced TNF-{alpha} production by primary human macrophages is mediated by phosphatidylinositol-3 (PI-3) kinase and mitogen-activated protein (MAP) kinase pathways J. Leukoc. Biol., October 1, 2005; 78(4): 1016 - 1023. [Abstract] [Full Text] [PDF]

				J. F. Foley, C.-R. Yu, R. Solow, M. Yacobucci, K. W. C. Peden, and J. M. Farber Roles for CXC Chemokine Ligands 10 and 11 in Recruiting CD4+ T Cells to HIV-1-Infected Monocyte-Derived Macrophages, Dendritic Cells, and Lymph Nodes J. Immunol., April 15, 2005; 174(8): 4892 - 4900. [Abstract] [Full Text] [PDF]

				L. Fantuzzi, C. Purificato, K. Donato, F. Belardelli, and S. Gessani Human Immunodeficiency Virus Type 1 gp120 Induces Abnormal Maturation and Functional Alterations of Dendritic Cells: a Novel Mechanism for AIDS Pathogenesis J. Virol., September 15, 2004; 78(18): 9763 - 9772. [Abstract] [Full Text] [PDF]

				J. E. Kirkley and F. A. Robey Inhibition of U937 Cytokine Secretion by HIV-1 gp120 C4-Derived Peptide Constructs Journal of Bioactive and Compatible Polymers, May 1, 2004; 19(3): 173 - 195. [Abstract] [PDF]

				A. M. Masci, M. Galgani, S. Cassano, S. De Simone, A. Gallo, V. De Rosa, S. Zappacosta, and L. Racioppi HIV-1 gp120 induces anergy in naive T lymphocytes through CD4-independent protein kinase-A-mediated signaling J. Leukoc. Biol., December 1, 2003; 74(6): 1117 - 1124. [Abstract] [Full Text]

				C. Lee, Q.-H. Liu, B. Tomkowicz, Y. Yi, B. D. Freedman, and R. G. Collman Macrophage activation through CCR5- and CXCR4-mediated gp120-elicited signaling pathways J. Leukoc. Biol., November 1, 2003; 74(5): 676 - 682. [Abstract] [Full Text] [PDF]

				L. Fantuzzi, F. Belardelli, and S. Gessani Monocyte/macrophage-derived CC chemokines and their modulation by HIV-1 and cytokines: A complex network of interactions influencing viral replication and AIDS pathogenesis J. Leukoc. Biol., November 1, 2003; 74(5): 719 - 725. [Abstract] [Full Text] [PDF]

				W. Choe, D. J. Volsky, and M. J. Potash Induction of Rapid and Extensive beta -Chemokine Synthesis in Macrophages by Human Immunodeficiency Virus Type 1 and gp120, Independently of Their Coreceptor Phenotype J. Virol., November 15, 2001; 75(22): 10738 - 10745. [Abstract] [Full Text] [PDF]

				M. Del Corno, Q.-H. Liu, D. Schols, E. de Clercq, S. Gessani, B. D. Freedman, and R. G. Collman HIV-1 gp120 and chemokine activation of Pyk2 and mitogen-activated protein kinases in primary macrophages mediated by calcium-dependent, pertussis toxin-insensitive chemokine receptor signaling Blood, November 15, 2001; 98(10): 2909 - 2916. [Abstract] [Full Text] [PDF]