| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
-Mediated Activation of Human Chondrocytes1
*
Division of Rheumatology, Scripps Clinic, La Jolla, CA 92037; and
Division of Arthritis Research, The Scripps Research Institute, La Jolla, CA 92037
Glucosamine represents one of the most commonly used drugs to treat
osteoarthritis. However, mechanisms of its antiarthritic activities are
still poorly understood. The present study identifies a novel mechanism
of glucosamine-mediated anti-inflammatory activity. It is shown
that both glucosamine and N-acetylglucosamine inhibit
IL-1
- and TNF-
-induced NO production in normal human articular
chondrocytes. The effect of the sugars on NO production is specific,
since several other monosaccharides, including glucose, glucuronic
acid, and N-acetylmannosamine, do not express this
activity. Furthermore, N-acetylglucosamine polymers,
including the dimer and the trimer, also do not affect NO production.
The observed suppression of IL-1-induced NO production is associated
with inhibition of inducible NO synthase mRNA and protein expression.
In addition, N-acetylglucosamine also suppresses the
production of IL-1-induced cyclooxygenase-2 and IL-6. The
constitutively expressed cyclooxygenase-1, however, was not affected by
the sugar. N-acetylglucosamine-mediated inhibition of
the IL-1 response of human chondrocytes was not associated with the
decreased inhibition of the mitogen-activated protein kinases c-Jun
N-terminal kinase, extracellular signal-related kinase, and p38, nor
with activation of the transcription factor NF-
B. In conclusion,
these results demonstrate that N-acetylglucosamine
expresses a unique range of activities and identifies a novel mechanism
for the inhibition of inflammatory processes.
This article has been cited by other articles:
|
|
 |
|
  Y.-H. Chang, C.-T. Horng, Y.-H. Chen, P.-L. Chen, C.-L. Chen, C.-M. Liang, M.-W. Chien, and J.-T. Chen Inhibitory Effects of Glucosamine on Endotoxin-Induced Uveitis in Lewis Rats Invest. Ophthalmol. Vis. Sci., December 1, 2008; 49(12): 5441 - 5449. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Xing, W. Feng, L. G. Not, A. P. Miller, Y. Zhang, Y.-F. Chen, E. Majid-Hassan, J. C. Chatham, and S. Oparil Increased protein O-GlcNAc modification inhibits inflammatory and neointimal responses to acute endoluminal arterial injury Am J Physiol Heart Circ Physiol, July 1, 2008; 295(1): H335 - H342. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-T. Chen, J.-B. Liang, C.-L. Chou, M.-W. Chien, R.-C. Shyu, P.-I Chou, and D.-W. Lu Glucosamine Sulfate Inhibits TNF-{alpha} and IFN-{gamma}-Induced Production of ICAM-1 in Human Retinal Pigment Epithelial Cells In Vitro Invest. Ophthalmol. Vis. Sci., February 1, 2006; 47(2): 664 - 672. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Verbruggen Chondroprotective drugs in degenerative joint diseases Rheumatology, February 1, 2006; 45(2): 129 - 138. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G.-X. Zhang, S. Yu, B. Gran, and A. Rostami Glucosamine Abrogates the Acute Phase of Experimental Autoimmune Encephalomyelitis by Induction of Th2 Response J. Immunol., December 1, 2005; 175(11): 7202 - 7208. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Yang, S. G. Carlson, D. McBurney, and W. E. Horton Jr. Multiple Signals Induce Endoplasmic Reticulum Stress in Both Primary and Immortalized Chondrocytes Resulting in Loss of Differentiation, Impaired Cell Growth, and Apoptosis J. Biol. Chem., September 2, 2005; 280(35): 31156 - 31165. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Bitler, T. M. Viale, B. Damaj, and R. Crea Hydrolyzed Olive Vegetation Water in Mice Has Anti-Inflammatory Activity J. Nutr., June 1, 2005; 135(6): 1475 - 1479. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A R Shikhman, D Amiel, D D'Lima, S-B Hwang, C Hu, A Xu, S Hashimoto, K Kobayashi, T Sasho, and M K Lotz Chondroprotective activity of N-acetylglucosamine in rabbits with experimental osteoarthritis Ann Rheum Dis, January 1, 2005; 64(1): 89 - 94. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. K. Sohanpal, S. El-Labany, M. Lahooti, J. A. Plumbridge, and I. C. Blomfield Integrated regulatory responses of fimB to N-acetylneuraminic (sialic) acid and GlcNAc in Escherichia coli K-12 PNAS, November 16, 2004; 101(46): 16322 - 16327. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. W. Poustie, J. Carran, K. McEleney, S. J. Dixon, T. P. Anastassiades, and S. M. Bernier N-Butyryl Glucosamine Increases Matrix Gene Expression by Chondrocytes J. Pharmacol. Exp. Ther., November 1, 2004; 311(2): 610 - 616. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ulrich-Vinther, M. D. Maloney, E. M. Schwarz, R. Rosier, and R. J. O'Keefe Articular Cartilage Biology J. Am. Acad. Ortho. Surg., November 1, 2003; 11(6): 421 - 430. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Pavelka, J. Gatterova, M. Olejarova, S. Machacek, G. Giacovelli, and L. C. Rovati Glucosamine Sulfate Use and Delay of Progression of Knee Osteoarthritis: A 3-Year, Randomized, Placebo-Controlled, Double-blind Study Arch Intern Med, October 14, 2002; 162(18): 2113 - 2123. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Ma, W. A. Rudert, J. Harnaha, M. Wright, J. Machen, R. Lakomy, S. Qian, L. Lu, P. D. Robbins, M. Trucco, et al. Immunosuppressive Effects of Glucosamine J. Biol. Chem., October 11, 2002; 277(42): 39343 - 39349. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Shikhman, D. C. Brinson, J. Valbracht, and M. K. Lotz Cytokine Regulation of Facilitated Glucose Transport in Human Articular Chondrocytes J. Immunol., December 15, 2001; 167(12): 7001 - 7008. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
