The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shimaoka, T.
Right arrow Articles by Yonehara, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shimaoka, T.
Right arrow Articles by Yonehara, S.
The Journal of Immunology, 2001, 166: 5108-5114.
Copyright © 2001 by The American Association of Immunologists

LOX-1 Supports Adhesion of Gram-Positive and Gram-Negative Bacteria

Takeshi Shimaoka*, Noriaki Kume1,{dagger}, Manabu Minami{dagger}, Kazutaka Hayashida{dagger}, Tatsuya Sawamura{ddagger}, Toru Kita{dagger} and Shin Yonehara*

* Institute for Virus Research and {dagger} Department of Geriatric Medicine, Graduate School of Medicine, Kyoto University, Kyoto, Japan; and {ddagger} Department of Bioscience, National Cardiovascular Center Research Institute, Suita, Japan

Adhesion of bacteria to vascular endothelial cells as well as mucosal cells and epithelial cells appears to be one of the initial steps in the process of bacterial infection, including infective endocarditis. We examined whether lectin-like oxidized low-density lipoprotein receptor 1 (LOX-1), a member of scavenger receptor family molecules with C-type lectin-like structure, can support adhesion of Gram-positive and Gram-negative bacteria. Chinese hamster ovary-K1 (CHO-K1) cells stably expressing LOX-1 can support binding of FITC-labeled Staphylococcus aureus and Escherichia coli, which was suppressed by poly(I) and an anti-LOX-1 mAb. Adhesion of these bacteria to LOX-1 does not require divalent cations or serum factors and can be supported under both static and nonstatic conditions. Cultured bovine aortic endothelial cells (BAEC) can also support adhesion of FITC-labeled S. aureus, which was similarly suppressed by poly(I) and an anti-LOX-1 mAb. In contrast, binding of FITC-labeled E. coli to BAEC was partially inhibited by the anti-LOX-1 mAb, and poly(I) did not block FITC-labeled E. coli adhesion to BAEC, but, rather, enhanced it under a static condition. TNF-{alpha} increased LOX-1-dependent adhesion of E. coli, but not that of S. aureus, suggesting that S. aureus adhesion to BAEC may require additional molecules, which cooperate with LOX-1 and suppressed by TNF-{alpha}. Taken together, LOX-1 can work as a cell surface receptor for Gram-positive and Gram-negative bacteria, such as S. aureus and E. coli, in a mechanism similar to that of class A scavenger receptors; however, other unknown molecules may also be involved in the adhesion of E. coli to BAEC, which is enhanced by poly(I).




This article has been cited by other articles:


Home page
 Clin. Chem.Home page
Y. Fujita, A. Kakino, N. Nishimichi, S. Yamaguchi, Y. Sato, S. Machida, L. Cominacini, Y. Delneste, H. Matsuda, and T. Sawamura
Oxidized LDL Receptor LOX-1 Binds to C-Reactive Protein and Mediates Its Vascular Effects
Clin. Chem., February 1, 2009; 55(2): 285 - 294.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
E. Herczenik and M. F. B. G. Gebbink
Molecular and cellular aspects of protein misfolding and disease
FASEB J, July 1, 2008; 22(7): 2115 - 2133.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J. E. Murphy, R. S. Vohra, S. Dunn, Z. G. Holloway, A. P. Monaco, S. Homer-Vanniasinkam, J. H. Walker, and S. Ponnambalam
Oxidised LDL internalisation by the LOX-1 scavenger receptor is dependent on a novel cytoplasmic motif and is regulated by dynamin-2
J. Cell Sci., July 1, 2008; 121(13): 2136 - 2147.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. Esen and T. Kielian
Central Role for MyD88 in the Responses of Microglia to Pathogen-Associated Molecular Patterns.
J. Immunol., June 1, 2006; 176(11): 6802 - 6811.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
J.-i. Hinagata, M. Kakutani, T. Fujii, T. Naruko, N. Inoue, Y. Fujita, J. L. Mehta, M. Ueda, and T. Sawamura
Oxidized LDL receptor LOX-1 is involved in neointimal hyperplasia after balloon arterial injury in a rat model
Cardiovasc Res, January 1, 2006; 69(1): 263 - 271.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
K. Inoue, Y. Arai, H. Kurihara, T. Kita, and T. Sawamura
Overexpression of Lectin-Like Oxidized Low-Density Lipoprotein Receptor-1 Induces Intramyocardial Vasculopathy in Apolipoprotein E-Null Mice
Circ. Res., July 22, 2005; 97(2): 176 - 184.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Park, F. G. Adsit, and J. C. Boyington
The 1.4 A Crystal Structure of the Human Oxidized Low Density Lipoprotein Receptor Lox-1
J. Biol. Chem., April 8, 2005; 280(14): 13593 - 13599.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Prevo, S. Banerji, J. Ni, and D. G. Jackson
Rapid Plasma Membrane-Endosomal Trafficking of the Lymph Node Sinus and High Endothelial Venule Scavenger Receptor/Homing Receptor Stabilin-1 (Feel-1/Clever-1)
J. Biol. Chem., December 10, 2004; 279(50): 52580 - 52592.
[Abstract] [Full Text] [PDF]

Home page
 Ann Rheum DisHome page
G F Ferraccioli and E Gremese
Autoantibodies and thrombophilia in RA: TNF{alpha} and TNF{alpha} blockers
Ann Rheum Dis, June 1, 2004; 63(6): 613 - 615.
[Full Text]

Home page
 J. Biol. Chem.Home page
A. S. J. Marshall, J. A. Willment, H.-H. Lin, D. L. Williams, S. Gordon, and G. D. Brown
Identification and Characterization of a Novel Human Myeloid Inhibitory C-type Lectin-like Receptor (MICL) That Is Predominantly Expressed on Granulocytes and Monocytes
J. Biol. Chem., April 9, 2004; 279(15): 14792 - 14802.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. Shimaoka, T. Nakayama, N. Kume, S. Takahashi, J. Yamaguchi, M. Minami, K. Hayashida, T. Kita, J. Ohsumi, O. Yoshie, et al.
Cutting Edge: SR-PSOX/CXC Chemokine Ligand 16 Mediates Bacterial Phagocytosis by APCs Through its Chemokine Domain
J. Immunol., August 15, 2003; 171(4): 1647 - 1651.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
P. D. Rogers, J. Thornton, K. S. Barker, D. O. McDaniel, G. S. Sacks, E. Swiatlo, and L. S. McDaniel
Pneumolysin-Dependent and -Independent Gene Expression Identified by cDNA Microarray Analysis of THP-1 Human Mononuclear Cells Stimulated by Streptococcus pneumoniae
Infect. Immun., April 1, 2003; 71(4): 2087 - 2094.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Honjo, K. Nakamura, K. Yamashiro, J. Kiryu, H. Tanihara, L. M. McEvoy, Y. Honda, E. C. Butcher, T. Masaki, and T. Sawamura
Lectin-like oxidized LDL receptor-1 is a cell-adhesion molecule involved in endotoxin-induced inflammation
PNAS, February 4, 2003; 100(3): 1274 - 1279.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
F. Coutant, L. Perrin-Cocon, S. Agaugue, T. Delair, P. Andre, and V. Lotteau
Mature Dendritic Cell Generation Promoted by Lysophosphatidylcholine
J. Immunol., August 15, 2002; 169(4): 1688 - 1695.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
M. Minami, N. Kume, T. Shimaoka, H. Kataoka, K. Hayashida, Y. Akiyama, I. Nagata, K. Ando, M. Nobuyoshi, M. Hanyuu, et al.
Expression of SR-PSOX, a Novel Cell-Surface Scavenger Receptor for Phosphatidylserine and Oxidized LDL in Human Atherosclerotic Lesions
Arterioscler Thromb Vasc Biol, November 1, 2001; 21(11): 1796 - 1800.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2001 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2001 by The American Association of Immunologists, Inc. All rights reserved.