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The Journal of Immunology, 2001, 166: 5058-5067.
Copyright © 2001 by The American Association of Immunologists

Identification of Novel Functional Regions Important for the Activity of HOXB7 in Mammalian Cells1

Yifah Yaron, Jeanne K. McAdara, Maureen Lynch, Elizabeth Hughes and Judith C. Gasson2

Division of Hematology-Oncology, Department of Medicine, Department of Biological Chemistry and Jonsson Comprehensive Cancer Center, University of California School of Medicine, Los Angeles, CA 90095

Members of the HOX family of homeobox transcription factors play a role in pattern formation in diverse developmental systems. The clearly documented role of HOX genes in the proliferation and differentiation of primary hematopoietic cells and cell lines provides a convenient system to pursue a biochemical analysis of HOX gene function in mammalian cells. To explore the role of HOXB7 in myeloid hematopoiesis, a number of mutations and deletions in the gene were constructed that targeted sequences with known functions or in regions that had not been examined previously. The wild-type and mutant B7 constructs were introduced into the murine myelomonocytic cell line, 32D, and assayed for their effects on G-CSF-induced myeloid differentiation. Wild-type HOXB7 inhibited the differentiation of 32D cells, whereas mutations in the Pbx-binding pentapeptide motif or the DNA-binding homeodomain, as well as internal deletions of the N-terminal unique region, blocked this effect. Interestingly, mutations eliminating two target sites for casein kinase II, the glutamate-rich C terminus, or the first 14 amino acids of HOXB7, led to enhanced 32D differentiation. A model proposing a role for these regions of HOXB7 is presented.




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