| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Antigen Presentation Research Group, Imperial College School of Medicine, Northwick Park Institute for Medical Research, and
St. Marks Hospital, Harrow, Middlesex, United Kingdom
Dendritic cells (DC) in the colon may regulate intestinal immunity
but remain poorly characterized. In this study a
CD11c+HLA-DR+lin-
(CD3-CD14-CD16-CD19-CD34-)
population has been identified by flow cytometry in cells obtained by
rapid collagenase digestion of human colonic and rectal biopsies. These
day 0 (d0) CD11c+HLA-DR+lin- cells
comprised 
0.6% of the mononuclear cells obtained from the lamina
propria, were endocytically active, and had the phenotype of immature
DC; they were CD40+ and expressed low levels of CD83 and
CD86, but little or no CD80 or CD25. Similar d0 DC populations were
isolated from the colonic mucosa of healthy controls and from both
inflamed and noninflamed tissue from patients with Crohn’s disease.
The lamina propria also contained a population of cells capable of
migrating out of biopsies during an overnight culture and
differentiating into mature DC with lower levels of endocytic activity
and high cell surface expression of CD40, CD80, CD86, CD83, and CD25.
This mature DC population was a potent stimulator of an allogeneic
mixed leukocyte (MLR). Overnight culture of cells isolated by enzymatic
digestion on d0 yielded DC with a phenotype intermediate between that
of the d0 cells and that of the cells migrating out overnight.
Overnight culture of colonic cells in which DC and
HLA-DR+lin+ cells were differentially labeled
with FITC-dextran suggested that some of the maturing DC might
differentiate from HLA-DR+lin+ progenitors.
This study presents the first analysis of the phenotype, maturational
status, and migratory activity of human gut DC.
This article has been cited by other articles:
|
|
 |
|
  D. A. Carlow, M. R. Gold, and H. J. Ziltener Lymphocytes in the Peritoneum Home to the Omentum and Are Activated by Resident Dendritic Cells J. Immunol., July 15, 2009; 183(2): 1155 - 1165. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Y. Salim, M. A. Silva, A. V. Keita, M. Larsson, P. Andersson, K.-E. Magnusson, M. H. Perdue, and J. D. Soderholm CD83+CCR7- Dendritic Cells Accumulate in the Subepithelial Dome and Internalize Translocated Escherichia coli HB101 in the Peyer's Patches of Ileal Crohn's Disease Am. J. Pathol., January 1, 2009; 174(1): 82 - 90. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Sun, P. W. Denton, J. D. Estes, F. A. Othieno, B. L. Wei, A. K. Wege, M. W. Melkus, A. Padgett-Thomas, M. Zupancic, A. T. Haase, et al. Intrarectal transmission, systemic infection, and CD4+ T cell depletion in humanized mice infected with HIV-1 J. Exp. Med., April 16, 2007; 204(4): 705 - 714. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Donaghy, J. Wilkinson, and A. L. Cunningham HIV interactions with dendritic cells: has our focus been too narrow? J. Leukoc. Biol., November 1, 2006; 80(5): 1001 - 1012. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Bedford, V. Todorovic, E. D. A. Westcott, A. C. J. Windsor, N. R. English, H. O. Al-Hassi, K. S. Raju, S. Mills, and S. C. Knight Adipose tissue of human omentum is a major source of dendritic cells, which lose MHC Class II and stimulatory function in Crohn's disease J. Leukoc. Biol., September 1, 2006; 80(3): 546 - 554. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J O Lindsay, K Whelan, A J Stagg, P Gobin, H O Al-Hassi, N Rayment, M A Kamm, S C Knight, and A Forbes Clinical, microbiological, and immunological effects of fructo-oligosaccharide in patients with Crohn's disease Gut, March 1, 2006; 55(3): 348 - 355. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P Middel, D Raddatz, B Gunawan, F Haller, and H-J Radzun Increased number of mature dendritic cells in Crohn's disease: evidence for a chemokine mediated retention mechanism Gut, February 1, 2006; 55(2): 220 - 227. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. B. Gurney, J. Elliott, H. Nassanian, C. Song, E. Soilleux, I. McGowan, P. A. Anton, and B. Lee Binding and Transfer of Human Immunodeficiency Virus by DC-SIGN+ Cells in Human Rectal Mucosa J. Virol., May 1, 2005; 79(9): 5762 - 5773. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B Eksteen, L S K Walker, and D H Adams Immune regulation and colitis: suppression of acute inflammation allows the development of chronic inflammatory bowel disease Gut, January 1, 2005; 54(1): 4 - 6. [Full Text] [PDF]
|
|
|
|
|
|
A L Hart, K Lammers, P Brigidi, B Vitali, F Rizzello, P Gionchetti, M Campieri, M A Kamm, S C Knight, and A J Stagg Modulation of human dendritic cell phenotype and function by probiotic bacteria Gut, November 1, 2004; 53(11): 1602 - 1609. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-F. Fonteneau, M. Larsson, A.-S. Beignon, K. McKenna, I. Dasilva, A. Amara, Y.-J. Liu, J. D. Lifson, D. R. Littman, and N. Bhardwaj Human Immunodeficiency Virus Type 1 Activates Plasmacytoid Dendritic Cells and Concomitantly Induces the Bystander Maturation of Myeloid Dendritic Cells J. Virol., May 15, 2004; 78(10): 5223 - 5232. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Karlsson, P. Larsson, A. E. Wold, and A. Rudin Pattern of Cytokine Responses to Gram-Positive and Gram-Negative Commensal Bacteria Is Profoundly Changed when Monocytes Differentiate into Dendritic Cells Infect. Immun., May 1, 2004; 72(5): 2671 - 2678. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Goddard, J. Youster, E. Morgan, and D. H. Adams Interleukin-10 Secretion Differentiates Dendritic Cells from Human Liver and Skin Am. J. Pathol., February 1, 2004; 164(2): 511 - 519. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A J Stagg, A L Hart, S C Knight, and M A Kamm The dendritic cell: its role in intestinal inflammation and relationship with gut bacteria Gut, October 1, 2003; 52(10): 1522 - 1529. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Gardner and A. Moffett Dendritic Cells in the Human Decidua Biol Reprod, October 1, 2003; 69(4): 1438 - 1446. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
