| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
E00-13 Institut National de la Recherche Scientifique (Institut National de la Santé et de la Recherche Médicale), Université Paris 6 and Laboratoire d’Immunologie Cellulaire et Immunopathologie de l’Ecole Pratique des Hautes Etudes, Paris, France;
Institut National de la Santé et de la Recherche Médicale Unité 494, hôpital Pitié-Salpêtrière, Paris, France;
Laboratoire d’Oncologie Virale, Institut Pasteur, Paris, France; and
Laboratoire de Biochimie and JER 3012 Associée à l’Agence Universitaire Francophone (AUPELF-UREF), Faculté des Sciences, Rabat, Morocco
Maturation of dendritic cells (DC) is known to result in decreased
capacity to produce HIV due to postentry block of its replicative
cycle. In this study, we compared the early phases of this cycle in
immature DC (iDC) and mature DC (mDC) generated from monocytes cultured
with GM-CSF and IL-4, trimeric CD40 ligand (DCCD40LT), or
monocyte-conditioned medium (DCMCM) being added or not from
day 5. Culture day 8 cells exposed to X4 HIV-1LAI or R5
HIV-1Ba-L were analyzed by semiquantitative R-U5 PCR,
which detects total HIV DNA. CXC chemokine receptor
4low (CXCR4low) CCR5+ iDC
harbored similar viral DNA amounts when exposed to either strain.
HIV-1LAI entered more efficiently into DCCD40LT
or DCMCM with up-regulated CXCR4. CCR5low
DCCD40LT still allowed entry of HIV-1Ba-L,
whereas CCR5- DCMCM displayed reduced
permissivity to this virus. Comparing amounts of late (long terminal
repeat (LTR)-gag PCR) and total (R-U5 PCR) viral DNA
products showed that HIV-1Ba-L reverse transcription was
more efficient than that of HIV-1LAI, but was not affected
by DC maturation. Southern blot detection of linear, circular, and
integrated HIV DNA showed that maturation affected neither HIV-1
nuclear import nor integration. When assessing virus transcription by
exposing iDC to pNL4-3.GFP or pNL4-3.Luc viruses pseudotyped with the G
protein of vesicular stomatitis virus (VSV-G), followed by culture with
or without CD40LT or MCM, GFP and luciferase activities decreased by
60–75% in mDC vs iDC. Thus, reduced HIV replication in mDC is
primarily due to a postintegration block occurring mainly at the
transcriptional level. We could not relate this block to altered
expression and nuclear localization of NF-
B proteins and SP1 and SP3
transcription factors.
This article has been cited by other articles:
|
|
 |
|
  I. Frank, H. Stossel, A. Gettie, S. G. Turville, J. W. Bess Jr., J. D. Lifson, I. Sivin, N. Romani, and M. Robbiani A Fusion Inhibitor Prevents Spread of Immunodeficiency Viruses, but Not Activation of Virus-Specific T Cells, by Dendritic Cells J. Virol., June 1, 2008; 82(11): 5329 - 5339. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Dong, A. M. Janas, J.-H. Wang, W. J. Olson, and L. Wu Characterization of Human Immunodeficiency Virus Type 1 Replication in Immature and Mature Dendritic Cells Reveals Dissociable cis- and trans-Infection J. Virol., October 15, 2007; 81(20): 11352 - 11362. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-H. Wang, A. M. Janas, W. J. Olson, and L. Wu Functionally Distinct Transmission of Human Immunodeficiency Virus Type 1 Mediated by Immature and Mature Dendritic Cells J. Virol., September 1, 2007; 81(17): 8933 - 8943. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. U. Cameron, A. J. Handley, D. C. Baylis, A. E. Solomon, N. Bernard, D. F. J. Purcell, and S. R. Lewin Preferential Infection of Dendritic Cells during Human Immunodeficiency Virus Type 1 Infection of Blood Leukocytes J. Virol., March 1, 2007; 81(5): 2297 - 2306. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Gilbert, C. Barat, R. Cantin, and M. J. Tremblay Involvement of Src and Syk Tyrosine Kinases in HIV-1 Transfer from Dendritic Cells to CD4+ T Lymphocytes J. Immunol., March 1, 2007; 178(5): 2862 - 2871. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Donaghy, J. Wilkinson, and A. L. Cunningham HIV interactions with dendritic cells: has our focus been too narrow? J. Leukoc. Biol., November 1, 2006; 80(5): 1001 - 1012. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. B. Gurney and C. H. Uittenbogaart Human Immunodeficiency Virus Persistence and Production in T-Cell Development Clin. Vaccine Immunol., November 1, 2006; 13(11): 1237 - 1245. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Holl, M. Peressin, S. Schmidt, T. Decoville, S. Zolla-Pazner, A.-M. Aubertin, and C. Moog Efficient inhibition of HIV-1 replication in human immature monocyte-derived dendritic cells by purified anti-HIV-1 IgG without induction of maturation Blood, June 1, 2006; 107(11): 4466 - 4474. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Cavrois, J. Neidleman, J. F. Kreisberg, D. Fenard, C. Callebaut, and W. C. Greene Human Immunodeficiency Virus Fusion to Dendritic Cells Declines as Cells Mature J. Virol., February 15, 2006; 80(4): 1992 - 1999. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Nobile, C. Petit, A. Moris, K. Skrabal, J.-P. Abastado, F. Mammano, and O. Schwartz Covert Human Immunodeficiency Virus Replication in Dendritic Cells and in DC-SIGN-Expressing Cells Promotes Long-Term Transmission to Lymphocytes J. Virol., May 1, 2005; 79(9): 5386 - 5399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Popov, A.-L. Chenine, A. Gruber, P.-L. Li, and R. M. Ruprecht Long-Term Productive Human Immunodeficiency Virus Infection of CD1a-Sorted Myeloid Dendritic Cells J. Virol., January 1, 2005; 79(1): 602 - 608. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Wilflingseder, B. Mullauer, H. Schramek, Z. Banki, M. Pruenster, M. P. Dierich, and H. Stoiber HIV-1-Induced Migration of Monocyte-Derived Dendritic Cells Is Associated with Differential Activation of MAPK Pathways J. Immunol., December 15, 2004; 173(12): 7497 - 7505. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Schiffer, C.-H. Lecellier, A. Mannioui, N. Felix, E. Nelson, J. Lehmann-Che, M.-L. Giron, J. C. Gluckman, A. Saib, and B. Canque Persistent Infection with Primate Foamy Virus Type 1 Increases Human Immunodeficiency Virus Type 1 Cell Binding via a Bet-Independent Mechanism J. Virol., October 15, 2004; 78(20): 11405 - 11410. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Emori, T. Ikeda, T. Ohashi, T. Masuda, T. Kurimoto, M. Takei, and M. Kannagi Inhibition of human immunodeficiency virus type 1 replication by Z-100, an immunomodulator extracted from human-type tubercle bacilli, in macrophages J. Gen. Virol., September 1, 2004; 85(9): 2603 - 2613. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Granelli-Piperno, A. Golebiowska, C. Trumpfheller, F. P. Siegal, and R. M. Steinman HIV-1-infected monocyte-derived dendritic cells do not undergo maturation but can elicit IL-10 production and T cell regulation PNAS, May 18, 2004; 101(20): 7669 - 7674. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Maranon, J.-F. Desoutter, G. Hoeffel, W. Cohen, D. Hanau, and A. Hosmalin Dendritic cells cross-present HIV antigens from live as well as apoptotic infected CD4+ T lymphocytes PNAS, April 20, 2004; 101(16): 6092 - 6097. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Poudrier, X. Weng, D. G. Kay, Z. Hanna, and P. Jolicoeur The AIDS-Like Disease of CD4C/Human Immunodeficiency Virus Transgenic Mice Is Associated with Accumulation of Immature CD11bHi Dendritic Cells J. Virol., November 1, 2003; 77(21): 11733 - 11744. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Turville, J. Wilkinson, P. Cameron, J. Dable, and A. L. Cunningham The role of dendritic cell C-type lectin receptors in HIV pathogenesis J. Leukoc. Biol., November 1, 2003; 74(5): 710 - 718. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Rohr, C. Marban, D. Aunis, and E. Schaeffer Regulation of HIV-1 gene transcription: from lymphocytes to microglial cells J. Leukoc. Biol., November 1, 2003; 74(5): 736 - 749. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bros, X.-L. Ross, A. Pautz, A. B. Reske-Kunz, and R. Ross The Human Fascin Gene Promoter Is Highly Active in Mature Dendritic Cells Due to a Stage-Specific Enhancer J. Immunol., August 15, 2003; 171(4): 1825 - 1834. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Trumpfheller, C. G. Park, J. Finke, R. M. Steinman, and A. Granelli-Piperno Cell type-dependent retention and transmission of HIV-1 by DC-SIGN Int. Immunol., February 1, 2003; 15(2): 289 - 298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. R. Clapham and A. McKnight Cell surface receptors, virus entry and tropism of primate lentiviruses J. Gen. Virol., August 1, 2002; 83(8): 1809 - 1829. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. D. Foley, M. Otero, J. M. Orenstein, R. J. Pomerantz, and M. J. Schnell Rhabdovirus-Based Vectors with Human Immunodeficiency Virus Type 1 (HIV-1) Envelopes Display HIV-1-Like Tropism and Target Human Dendritic Cells J. Virol., January 1, 2002; 76(1): 19 - 31. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
