The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sano, H.
Right arrow Articles by Leff, A. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sano, H.
Right arrow Articles by Leff, A. R.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH
The Journal of Immunology, 2001, 166: 3515-3521.
Copyright © 2001 by The American Association of Immunologists

Extracellular Signal-Regulated Kinase 1/2-Mediated Phosphorylation of Cytosolic Phospholipase A2 Is Essential for Human Eosinophil Adhesion to Fibronectin1

Hiroyuki Sano*, Xiangdong Zhu*, Akiko Sano*, Evan E. Boetticher*, Takanobu Shioya{ddagger}, Benjamin Jacobs*, Nilda M. Munoz* and Alan R. Leff2,*,{dagger}

* Section of Pulmonary and Critical Care Medicine, Department of Medicine and {dagger} Department of Neurobiology, Pharmacological and Physiology, Pediatrics, Anesthesia and Critical Care, and Committees on Clinical Pharmacology and Cell Physiology, Division of Biological Sciences, University of Chicago, Chicago, IL 60637; and {ddagger} Akita University College of Allied Medical Science, Akita, Japan

We examined the role of p38, p42, and p44 mitogen-activated protein kinase (MAPK) isoforms and cytosolic phospholipase A2 (cPLA2) activation in human eosinophil adhesion to plate-coated fibronectin (FN). In the control state, eosinophil adhesion was maximal, with 10 µg/ml FN at 30 min, and decreased after 60–90 min. Western blot analysis demonstrated that p44/42 MAPK (extracellular signal-regulated kinase (ERK)1/2) and cPLA2 were phosphorylated during adhesion to FN, whereas p38 MAPK phosphorylation was unchanged. Preincubation of eosinophils with U0126 or PD98059, two structurally unrelated MAPK kinase inhibitors, or arachidonic trifluoromethyl ketone, a cPLA2 inhibitor, blocked eosinophil adhesion to FN. By contrast, eosinophil adhesion was unaffected by SB203580, a p38 MAPK inhibitor. Pretreatment of eosinophils with okadaic acid, a serine/threonine phosphatase inhibitor, at the concentrations that induced ERK1/2 and cPLA2 phosphorylation caused an increase in maximal eosinophil adhesion to FN for >60 min. MAPK kinase inhibition but not p38 inhibition also blocked FN-mediated F-actin redistribution in eosinophils and prevented cPLA2 phosphorylation caused by adhesion to FN. These results demonstrate that ERK1/2 mediating cPLA2 activation is essential for eosinophil adhesion to FN.




This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
M. Mashimo, T. Hirabayashi, T. Murayama, and T. Shimizu
Cytosolic PLA2{alpha} activation in Purkinje neurons and its role in AMPA-receptor trafficking
J. Cell Sci., September 15, 2008; 121(18): 3015 - 3024.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. H. Ulfman, V. M. Kamp, C. W. van Aalst, L. P. Verhagen, M. E. Sanders, K. A. Reedquist, M. Buitenhuis, and L. Koenderman
Homeostatic Intracellular-Free Ca2+ Is Permissive for Rap1-Mediated Constitutive Activation of {alpha}4 Integrins on Eosinophils
J. Immunol., April 15, 2008; 180(8): 5512 - 5519.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. M. Munoz, A. Y. Meliton, A. Lambertino, E. Boetticher, J. Learoyd, F. Sultan, X. Zhu, W. Cho, and A. R. Leff
Transcellular Secretion of Group V Phospholipase A2 from Epithelium Induces beta2-Integrin-Mediated Adhesion and Synthesis of Leukotriene C4 in Eosinophils
J. Immunol., July 1, 2006; 177(1): 574 - 582.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
S. Myo, X. Zhu, S. Myou, A.Y. Meliton, J. Liu, E. Boetticher, A.T. Lambertino, C. Xu, N.M. Munoz, and A.R. Leff
Additive blockade of {beta}2-integrin adhesion of eosinophils by salmeterol and fluticasone propionate
Eur. Respir. J., April 1, 2004; 23(4): 511 - 517.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. M. Sandberg, X. Ma, D. VonDerLinden, M. D. Godeny, and P. P. Sayeski
Jak2 Tyrosine Kinase Mediates Angiotensin II-dependent Inactivation of ERK2 via Induction of Mitogen-activated Protein Kinase Phosphatase 1
J. Biol. Chem., January 16, 2004; 279(3): 1956 - 1967.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Esnault and J. S. Malter
Hyaluronic Acid or TNF-{alpha} Plus Fibronectin Triggers Granulocyte Macrophage-Colony-Stimulating Factor mRNA Stabilization in Eosinophils Yet Engages Differential Intracellular Pathways and mRNA Binding Proteins
J. Immunol., December 15, 2003; 171(12): 6780 - 6787.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
A. Holub, J. Byrnes, S. Anderson, L. Dzaidzio, N. Hogg, and A. Huttenlocher
Ligand density modulates eosinophil signaling and migration
J. Leukoc. Biol., May 1, 2003; 73(5): 657 - 664.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
X. Zhu, B. Jacobs, E. Boetticher, S. Myou, A. Meliton, H. Sano, A. T. Lambertino, N. M. Munoz, and A. R. Leff
IL-5-induced integrin adhesion of human eosinophils caused by ERK1/2-mediated activation of cPLA2
J. Leukoc. Biol., November 1, 2002; 72(5): 1046 - 1053.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Vliagoftis
Thrombin Induces Mast Cell Adhesion to Fibronectin: Evidence for Involvement of Protease-Activated Receptor-1
J. Immunol., October 15, 2002; 169(8): 4551 - 4558.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Myou, X. Zhu, E. Boetticher, S. Myo, A. Meliton, A. Lambertino, N. M. Munoz, and A. R. Leff
Blockade of Focal Clustering and Active Conformation in {beta}2-Integrin-Mediated Adhesion of Eosinophils to Intercellular Adhesion Molecule-1 Caused by Transduction of HIV TAT-Dominant Negative Ras
J. Immunol., September 1, 2002; 169(5): 2670 - 2676.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Hoyos, A. Imam, I. Korichneva, E. Levi, R. Chua, and U. Hammerling
Activation of c-Raf Kinase by Ultraviolet Light. REGULATION BY RETINOIDS
J. Biol. Chem., June 21, 2002; 277(26): 23949 - 23957.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Cussac, S. Schaak, C. Denis, and H. Paris
alpha 2B-Adrenergic Receptor Activates MAPK via a Pathway Involving Arachidonic Acid Metabolism, Matrix Metalloproteinases, and Epidermal Growth Factor Receptor Transactivation
J. Biol. Chem., May 24, 2002; 277(22): 19882 - 19888.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. Esnault and J. S. Malter
Extracellular signal-regulated kinase mediates granulocyte-macrophage colony-stimulating factor messenger RNA stabilization in tumor necrosis factor-alpha plus fibronectin-activated peripheral blood eosinophils
Blood, May 13, 2002; 99(11): 4048 - 4052.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2001 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2001 by The American Association of Immunologists, Inc. All rights reserved.