HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kacani, L. Articles by Dierich, M. P. Search for Related Content PubMed PubMed Citation Articles by Kacani, L. Articles by Dierich, M. P.

C5a and C5a^desArg Enhance the Susceptibility of Monocyte-Derived Macrophages to HIV Infection¹

Laco Kacani^2,*, Zoltán Bánki^*, Jörg Zwirner, Harald Schennach, Zsuzsa Bajtay, Anna Erdei, Heribert Stoiber^* and Manfred P. Dierich^*

^* Institut für Hygiene und Sozialmedizin, Leopold-Franzens-Universität, Ludwig-Boltzmann-Institut für AIDS-Forschung, Innsbruck, Austria; Department of Immunology, University of Göttingen, Göttingen, Germany; Central Institute for Blood Transfusion, General Hospital, Innsbruck, Austria; and Department of Immunology, Eötvös Loránd University, Budapest, Hungary

Mononuclear phagocytes, which include circulating blood monocytes and differentiated tissue macrophages, are believed to play a central role in the sexual transmission of HIV infection. The ability of HIV to productively infect these cells may be influenced by action of exogenous or host-derived substances at the site of viral entry. Given the potent capacities of inflammatory mediators to stimulate anaphylatoxic and immunomodulatory functions in mucosa, the effects of complement-derived anaphylatoxins on the susceptibility of monocytes and monocyte-derived macrophages (MDM) to HIV-1 infection were examined. In our in vitro system, the susceptibility to infection was up to 40 times increased in MDM that had been exposed to C5a or C5a^desArg, but not to C3a or C3a^desArg, for 2 days before adding of virus. By contrast, the treatment with complement anaphylatoxins did not affect HIV replication in fresh monocytes. Stimulatory effect of C5a and its desArg derivative on HIV infection correlated with the increase of TNF- and IL-6 secretion from MDM. All these functional effects of C5a and C5a^desArg were reversible by treatment of cells with the mAb that functionally blocks C5aR. Taken together, these results indicate that C5a and C5a^desArg may increase the susceptibility of MDM to HIV infection through stimulation of TNF- and IL-6 secretion from these cells.

This article has been cited by other articles:

				Y. Terao, M. Yamaguchi, S. Hamada, and S. Kawabata Multifunctional Glyceraldehyde-3-phosphate Dehydrogenase of Streptococcus pyogenes Is Essential for Evasion from Neutrophils J. Biol. Chem., May 19, 2006; 281(20): 14215 - 14223. [Abstract] [Full Text] [PDF]

				H. Boshra, J. Li, R. Peters, J. Hansen, A. Matlapudi, and J. O. Sunyer Cloning, Expression, Cellular Distribution, and Role in Chemotaxis of a C5a Receptor in Rainbow Trout: The First Identification of a C5a Receptor in a Nonmammalian Species J. Immunol., April 1, 2004; 172(7): 4381 - 4390. [Abstract] [Full Text] [PDF]