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Immature Multipotent Hemopoietic Progenitors Lacking Long-Term Bone Marrow-Reconstituting Activity in the Aorta-Gonad-Mesonephros Region of Murine Day 10 Fetuses¹

Koichiro Ohmura^*,, Hiroshi Kawamoto^*, Min Lu^*, Tomokatsu Ikawa^*, Shoichi Ozaki, Kazuwa Nakao and Yoshimoto Katsura^2,*

^* Department of Immunology, Institute for Frontier Medical Sciences, and Department of Medicine and Clinical Science, Faculty of Medicine, Kyoto University, Kyoto, Japan

Previous studies indicated that multipotent progenitors exist in early fetuses that do not contain long-term reconstituting (LTR) activity. However, it remained unclear whether these multipotent progenitors are committed to the hemopoietic lineage or are immature mesodermal cells or hemangioblasts. In this study, we have succeeded in enriching the multipotent progenitors that are capable of generating myeloid, T, and B cells in the LFA-1^- subpopulation of TER-119^-c-kit⁺CD45⁺ cells from the aorta-gonad-mesonephros (AGM) region of day 10 fetuses. We found that these day 10 AGM LFA-1^- cells do not show the LTR activity, whereas day 11 AGM LFA-1^- cells do have such an activity. These results strongly suggest that multipotent progenitors lacking LTR activity emerge as CD45⁺ hemopoietic progenitor cells in the AGM region on the 10th day of gestation, and such p-Multi mature into hemopoietic stem cells by acquiring LTR activity.

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