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Phenotypic Distinction and Functional Characterization of Pro-B Cells in Adult Mouse Bone Marrow¹

Mariluz P. Mojica^*, S. Scott Perry, A. Elena Searles, Kojo S. J. Elenitoba-Johnson, L. Jeanne Pierce, Anne Wiesmann, William B. Slayton^¶ and Gerald J. Spangrude^,,

Departments of ^* Human Genetics, Pathology, Medicine (Division of Hematology), Oncological Sciences, and ^¶ Pediatrics, University of Utah, Salt Lake City, UT 84132

A lymphoid-committed progenitor population was isolated from mouse bone marrow based on the cell surface phenotype Thy-1.1^negSca-1^posc-Kit^lowLin^neg. These cells were CD43^posCD24^pos on isolation and proliferated in response to the cytokine combination of steel factor, IL-7, and Flt3 ligand. Lymphoid-committed progenitors could be segregated into more primitive and more differentiated subsets based on expression of AA4.1. The more differentiated subset generated only B lymphoid cells in 92% of total colonies assayed, lacked T lineage potential, and expressed Pax5. These studies have therefore defined and isolated a B lymphoid-committed progenitor population at a developmental stage corresponding to the initial expression of CD45R.

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