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*
Meakins-Christie Laboratories, McGill University, Montreal, Quebec, Canada;
Centre hospitalier de l’Université de Montreal, Notre-Dame Hospital, Montreal, Quebec, Canada; and
Magainin Institute of Molecular Medicine, Magainin Pharmaceuticals Inc., Plymouth, PA 19462
Human polymorphonuclear neutrophils (PMNs) express surface
receptors for various inflammatory mediators, including IgE and IL-4.
Recently, the IL-9R locus has been genetically linked to asthma and
bronchial hyperresponsiveness in humans. In this study, we evaluated
expression of the IL-9R and the effect of IL-9 on human PMNs. RT-PCR
analysis showed the presence of IL-9R
-chain mRNA in PMN RNA
preparations from asthmatic patients. Using FACS analysis, surface
expression of IL-9R was detected on PMNs freshly isolated from
asthmatics, and to a lesser extent on normal controls. In addition,
protein expression of IL-9R was also detected in peripheral blood
and bronchoalveolar lavage PMNs. Furthermore, functional studies showed
that IL-9 stimulation of PMNs results in the release of IL-8 in a
concentration-dependent manner. The anti-IL-9 neutralizing Ab
suppressed this effect, but had no effect on GM-CSF-induced IL-8
release from PMNs. Taken together, these findings suggest a novel role
for PMNs in allergic disease through the expression and activation of
the IL-9R.
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