HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Le, P. T. Articles by Ellis, T. M. Search for Related Content PubMed PubMed Citation Articles by Le, P. T. Articles by Ellis, T. M. Pubmed/NCBI databases Substance via MeSH Medline Plus Health Information Stem Cells

Human Thymic Epithelial Cells Inhibit IL-15- and IL-2-Driven Differentiation of NK Cells from the Early Human Thymic Progenitors¹

Phong T. Le^2,*, Kimberly L. Adams^*, Ninef Zaya, Herbert L. Mathews, Walter J. Storkus^¶ and Thomas M. Ellis^,§

^* Department of Cell Biology, Neurobiology, and Anatomy and the Program in Immunology for Aging, Stritch School of Medicine, and Departments of Microbiology and Immunology and ^§ Medicine, Loyola University Chicago Medical Center, Maywood, IL 60153; and ^¶ Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261

T/NK progenitors are present in the thymus; however, the thymus predominantly promotes T cell development. In this study, we demonstrated that human thymic epithelial cells (TEC) inhibit NK cell development. Most ex vivo human thymocytes express CD1a, indicating that thymic progenitors are predominantly committed to the T cell lineage. In contrast, the CD1a^-CD3^-CD56⁺ NK population comprises only 0.2% (n = 7) of thymocytes. However, we observed increases in the percentage (20- to 25-fold) and absolute number (13- to 71-fold) of NK cells when thymocytes were cultured with mixtures of either IL-2, IL-7, and stem cell factor or IL-15, IL-7, and stem cell factor. TEC, when present in the cultures, inhibited the increases in the percentage (3- to 10-fold) and absolute number (3- to 25-fold) of NK cells. Furthermore, we show that TEC-derived soluble factors inhibit generation of NK-CFU and inhibit IL15- or IL2-driven NK cell differentiation from thymic CD34⁺ triple-negative thymocytes. The inhibitory activity was found to be associated with a 8,000- to 30,000 Da fraction. Thus, our data demonstrate that TEC inhibit NK cell development from T/NK CD34⁺ triple negative progenitors via soluble factor(s), suggesting that the human thymic microenvironment not only actively promotes T cell maturation but also controls the development of non-T lineage cells such as the NK lineage.