| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of
*
Medicine and
Microbiology and Immunology, Division of Infectious Disease, Albert Einstein College of Medicine, Bronx, NY 10461
The IgM mAbs 12A1 and 13F1 are protective and nonprotective, respectively, against lethal Cryptococcus neoformans infection in mice. To better understand the variables that contribute to IgM efficacy against C. neoformans, we studied the effects of inoculum size, route of infection, and Ab dose for each of these mAbs. mAb 13F1 did not prolong survival under any condition studied. mAb 12A1 prolonged survival after the administration of certain Ab doses after i.p. infection with defined inocula and promoted phagocytosis, agglutination, and the formation of inflammatory cell rings around yeast cells in vivo. Large Ab doses of mAb 12A1 resulted in either no protection or enhanced infection, consistent with a prozone-like effect. Investigation of this phenomenon revealed that the fungal cell was protected against microbicidal nitrogen-derived oxidants when large amounts of Ab were bound to the C. neoformans capsule. mAb 12A1 was opsonic in vitro for peritoneal, but not splenic or alveolar macrophages. In summary, our results indicate that IgM efficacy against C. neoformans is a function of the route of infection, inoculum, and Ab dose and is associated with its ability to promote opsonization, agglutination, and phagocytic ring formation in vivo. The occurrence of the prozone-like phenomenon implies that high Ab titers are not necessarily beneficial in assuring protection against certain pathogens and that caution should be exercised in using high Ab titer as a measure for vaccine efficacy.
This article has been cited by other articles:
|
|
 |
|
  R. Buissa-Filho, R. Puccia, A. F. Marques, F. A. Pinto, J. E. Munoz, J. D. Nosanchuk, L. R. Travassos, and C. P. Taborda The Monoclonal Antibody against the Major Diagnostic Antigen of Paracoccidioides brasiliensis Mediates Immune Protection in Infected BALB/c Mice Challenged Intratracheally with the Fungus Infect. Immun., July 1, 2008; 76(7): 3321 - 3328. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Rodrigues, L. Shi, E. Barreto-Bergter, L. Nimrichter, S. E. Farias, E. G. Rodrigues, L. R. Travassos, and J. D. Nosanchuk Monoclonal Antibody to Fungal Glucosylceramide Protects Mice against Lethal Cryptococcus neoformans Infection Clin. Vaccine Immunol., October 1, 2007; 14(10): 1372 - 1376. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Zaragoza, M. Alvarez, A. Telzak, J. Rivera, and A. Casadevall The Relative Susceptibility of Mouse Strains to Pulmonary Cryptococcus neoformans Infection Is Associated with Pleiotropic Differences in the Immune Response Infect. Immun., June 1, 2007; 75(6): 2729 - 2739. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Macura, T. Zhang, and A. Casadevall Dependence of Macrophage Phagocytic Efficacy on Antibody Concentration Infect. Immun., April 1, 2007; 75(4): 1904 - 1915. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. R. Kozel, P. Thorkildson, S. Brandt, W. H. Welch, J. A. Lovchik, D. P. AuCoin, J. Vilai, and C. R. Lyons Protective and Immunochemical Activities of Monoclonal Antibodies Reactive with the Bacillus anthracis Polypeptide Capsule Infect. Immun., January 1, 2007; 75(1): 152 - 163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Mulgrew, K. Kinneer, X.-T. Yao, B. K. Ward, M. M. Damschroder, B. Walsh, S.-Y. Mao, C. Gao, P. A. Kiener, S. Coats, et al. Direct targeting of {alpha}v{beta}3 integrin on tumor cells with a monoclonal antibody, AbegrinTM Mol. Cancer Ther., December 1, 2006; 5(12): 3122 - 3129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Sevilla, B. Robledo, A. Rementeria, M. D. Moragues, and J. Ponton A Fungicidal Monoclonal Antibody Protects against Murine Invasive Candidiasis. Infect. Immun., May 1, 2006; 74(5): 3042 - 3045. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C. McFadden, M. De Jesus, and A. Casadevall The Physical Properties of the Capsular Polysaccharides from Cryptococcus neoformans Suggest Features for Capsule Construction J. Biol. Chem., January 27, 2006; 281(4): 1868 - 1875. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. E. McClelland, W. T. Perrine, W. K. Potts, and A. Casadevall Relationship of Virulence Factor Expression to Evolved Virulence in Mouse-Passaged Cryptococcus neoformans Lines Infect. Immun., October 1, 2005; 73(10): 7047 - 7050. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Subramaniam, N. French, and L.-a. Pirofski Cryptococcus neoformans-Reactive and Total Immunoglobulin Profiles of Human Immunodeficiency Virus-Infected and Uninfected Ugandans Clin. Vaccine Immunol., October 1, 2005; 12(10): 1168 - 1176. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Burns, M. Abadi, and L.-a. Pirofski Modulation of the Lung Inflammatory Response to Serotype 8 Pneumococcal Infection by a Human Immunoglobulin M Monoclonal Antibody to Serotype 8 Capsular Polysaccharide Infect. Immun., August 1, 2005; 73(8): 4530 - 4538. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Torres, R. May, M. D. Scharff, and A. Casadevall Variable-Region-Identical Antibodies Differing in Isotype Demonstrate Differences in Fine Specificity and Idiotype J. Immunol., February 15, 2005; 174(4): 2132 - 2142. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. J. Brady Antibody-Mediated Immunomodulation: a Strategy To Improve Host Responses against Microbial Antigens Infect. Immun., February 1, 2005; 73(2): 671 - 678. [Full Text] [PDF]
|
|
|
|
 |
|
 B. C. Fries, E. Cook, X. Wang, and A. Casadevall Effects of Antifungal Interventions on the Outcome of Experimental Infections with Phenotypic Switch Variants of Cryptococcus neoformans Antimicrob. Agents Chemother., January 1, 2005; 49(1): 350 - 357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. W. Maitta, K. Datta, Q. Chang, R. X. Luo, B. Witover, K. Subramaniam, and L.-a. Pirofski Protective and Nonprotective Human Immunoglobulin M Monoclonal Antibodies to Cryptococcus neoformans Glucuronoxylomannan Manifest Different Specificities and Gene Use Profiles Infect. Immun., August 1, 2004; 72(8): 4810 - 4818. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. W. Maitta, K. Datta, A. Lees, S. S. Belouski, and L.-a. Pirofski Immunogenicity and Efficacy of Cryptococcus neoformans Capsular Polysaccharide Glucuronoxylomannan Peptide Mimotope-Protein Conjugates in Human Immunoglobulin Transgenic Mice Infect. Immun., January 1, 2004; 72(1): 196 - 208. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. R. Kozel, R. S. MacGill, A. Percival, and Q. Zhou Biological Activities of Naturally Occurring Antibodies Reactive with Candida albicans Mannan Infect. Immun., January 1, 2004; 72(1): 209 - 218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Burns, Z. Zhong, M. Steinitz, and L.-a. Pirofski Modulation of Polymorphonuclear Cell Interleukin-8 Secretion by Human Monoclonal Antibodies to Type 8 Pneumococcal Capsular Polysaccharide Infect. Immun., December 1, 2003; 71(12): 6775 - 6783. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Dadachova, A. Nakouzi, R. A. Bryan, and A. Casadevall Ionizing radiation delivered by specific antibody is therapeutic against a fungal infection PNAS, September 16, 2003; 100(19): 10942 - 10947. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Netski and T. R. Kozel Fc-Dependent and Fc-Independent Opsonization of Cryptococcus neoformans by Anticapsular Monoclonal Antibodies: Importance of Epitope Specificity Infect. Immun., June 1, 2002; 70(6): 2812 - 2819. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Shapiro, D. O. Beenhouwer, M. Feldmesser, C. Taborda, M. C. Carroll, A. Casadevall, and M. D. Scharff Immunoglobulin G Monoclonal Antibodies to Cryptococcus neoformans Protect Mice Deficient in Complement Component C3 Infect. Immun., May 1, 2002; 70(5): 2598 - 2604. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
