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Chains Associated with the V
14 Semi-Invariant TCR -Chain in the Selection of CD4+ NK T Cells1
*
Unité de Biologie Moléculaire du Gène, Département d’Immunologie, Institut Pasteur; and
Hôpital Necker, Paris, France
The NK1.1+TCR
int CD4+, or
double negative T cells (NK T cells) consist of a mixture of
CD1d-restricted and CD1d-unrestricted cells. The relationships between
CD4+NK1.1+ T cells and conventional T cells are
not understood. To compare their respective TCR repertoires,
NK1.1+TCRint, CD4+ T cells
have been sorted out of the thymus, liver, spleen, and bone marrow of
C57BL/6 mice. Molecular analysis showed that thymus and liver used
predominantly the V14-J281 and V 2, 7, and 8 segments. These
cells are CD1d restricted and obey the original definition of NK T
cells. The complementarity-determining region 3 (CDR3) sequences of the
TCR V8.2-J2.5 chain of liver and thymus CD4+ NK T
cells were determined and compared with those of the same
rearrangements of conventional CD4+ T cells. No amino acid
sequence or usage characteristic of NK T cells could be evidenced: the
V8.2-J2.5 diversity regions being primarily the same in NK T and
in T cells. No clonal expansion of the -chains was observed in
thymus and liver CD1d-restricted CD4+NK T cells, suggesting
the absence of acute or chronic Ag-driven stimulation. Molecular
analysis of the TCR used by V14-J281 transgenic mice on a
C-/- background showed that the -chain can
associate with -chains using any V segment, except in NK T cells
in which it paired predominately with V 2, 7, and 8+
-chains. The structure of the TCR of NK T cells thus reflects the
affinity for the CD1d molecule rather than a structural constraint
leading to the association of the invariant -chain with a
distinctive subset of V segment.
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