| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
CUTTING EDGE |
*
Division of Clinical Immunology and Allergy, Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224;
Division of Otolaryngology, University of Chicago, Chicago, IL; and
LeukoSite Inc., Boston, MA 02142
Chemokine-induced eosinophil chemotaxis is
mediated primarily through the C-C chemokine receptor, CCR3. We have
now detected CCR3 immunoreactivity on epithelial cells in biopsies of
patients with asthma and other respiratory diseases. CCR3 mRNA was
detected by Northern blot analysis after TNF-
stimulation of the
human primary bronchial epithelial cells as well as the epithelial cell
line, BEAS-2B; IFN-
potentiated the TNF--induced expression.
Western blots and flow cytometry confirmed the expression of CCR3
protein. This receptor is functional based on studies demonstrating
eotaxin-induced intracellular Ca2+ flux and tyrosine
phosphorylation of cellular proteins. The specificity
of this functional response was confirmed by blocking these signaling
events with anti-CCR3 mAb (7B11) or pertussis toxin. Furthermore,
125I-eotaxin binding assay confirmed that CCR3 expressed on
epithelial cells have the expected ligand specificity. These studies
indicate that airway epithelial cells express CCR3 and suggest that
CCR3 ligands may influence epithelial cell
functions.
This article has been cited by other articles:
|
|
 |
|
  A. Ricciardi, A. R. Elia, P. Cappello, M. Puppo, C. Vanni, P. Fardin, A. Eva, D. Munroe, X. Wu, M. Giovarelli, et al. Transcriptome of Hypoxic Immature Dendritic Cells: Modulation of Chemokine/Receptor Expression Mol. Cancer Res., February 1, 2008; 6(2): 175 - 185. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Joubert, S. Lajoie-Kadoch, M. Welman, S. Dragon, S. Letuvee, B. Tolloczko, A. J. Halayko, A. S. Gounni, K. Maghni, and Q. Hamid Expression and Regulation of CCR1 by Airway Smooth Muscle Cells in Asthma J. Immunol., January 15, 2008; 180(2): 1268 - 1275. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Mori, K. Ogawa, K. Someya, Y. Kunori, D. Nagakubo, O. Yoshie, F. Kitamura, T. Hiroi, and O. Kaminuma Selective suppression of Th2-mediated airway eosinophil infiltration by low-molecular weight CCR3 antagonists Int. Immunol., August 1, 2007; 19(8): 913 - 921. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. C. Fulkerson, C. A. Fischetti, M. L. McBride, L. M. Hassman, S. P. Hogan, and M. E. Rothenberg A central regulatory role for eosinophils and the eotaxin/CCR3 axis in chronic experimental allergic airway inflammation PNAS, October 31, 2006; 103(44): 16418 - 16423. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Beck, B. Tancowny, M. E. Brummet, S. Y. Asaki, S. L. Curry, M. B. Penno, M. Foster, A. Bahl, and C. Stellato Functional Analysis of the Chemokine Receptor CCR3 on Airway Epithelial Cells. J. Immunol., September 1, 2006; 177(5): 3344 - 3354. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. O. Abonyo, M. S. Alexander, and A. S. Heiman Autoregulation of CCL26 synthesis and secretion in A549 cells: a possible mechanism by which alveolar epithelial cells modulate airway inflammation Am J Physiol Lung Cell Mol Physiol, September 1, 2005; 289(3): L478 - L488. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Joubert, S. Lajoie-Kadoch, I. Labonte, A. S. Gounni, K. Maghni, V. Wellemans, J. Chakir, M. Laviolette, Q. Hamid, and B. Lamkhioued CCR3 Expression and Function in Asthmatic Airway Smooth Muscle Cells J. Immunol., August 15, 2005; 175(4): 2702 - 2708. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. E. John, M. S. Thomas, A. A. Berlin, and N. W. Lukacs Temporal Production of CCL28 Corresponds to Eosinophil Accumulation and Airway Hyperreactivity in Allergic Airway Inflammation Am. J. Pathol., February 1, 2005; 166(2): 345 - 353. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Tager, R. L. Kradin, P. LaCamera, S. D. Bercury, G. S. V. Campanella, C. P. Leary, V. Polosukhin, L.-H. Zhao, H. Sakamoto, T. S. Blackwell, et al. Inhibition of Pulmonary Fibrosis by the Chemokine IP-10/CXCL10 Am. J. Respir. Cell Mol. Biol., October 1, 2004; 31(4): 395 - 404. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. G. Kelsen, M. O. Aksoy, Y. Yang, S. Shahabuddin, J. Litvin, F. Safadi, and T. J. Rogers The chemokine receptor CXCR3 and its splice variant are expressed in human airway epithelial cells Am J Physiol Lung Cell Mol Physiol, September 1, 2004; 287(3): L584 - L591. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. B. Kodali, W. J.H. Kim, I. I. Galaria, C. Miller, A. D. Schecter, S. A. Lira, and M. B. Taubman CCL11 (Eotaxin) Induces CCR3-Dependent Smooth Muscle Cell Migration Arterioscler Thromb Vasc Biol, July 1, 2004; 24(7): 1211 - 1216. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Oostendorp, M. N. Hylkema, M. Luinge, M. Geerlings, H. Meurs, W. Timens, J. Zaagsma, D. S. Postma, H. W. Boddeke, and K. Biber Localization and Enhanced mRNA Expression of the Orphan Chemokine Receptor L-CCR in the Lung in a Murine Model of Ovalbumin-induced Airway Inflammation J. Histochem. Cytochem., March 1, 2004; 52(3): 401 - 410. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Zhang, M. P. Soares, Y. Guan, S. Matheravidathu, R. Wnek, K. E. Johnson, A. Meisher, S. A. Iliff, J. S. Mudgett, M. S. Springer, et al. Functional Expression and Characterization of Macaque C-C Chemokine Receptor 3 (CCR3) and Generation of Potent Antagonistic Anti-macaque CCR3 Monoclonal Antibodies J. Biol. Chem., September 6, 2002; 277(37): 33799 - 33810. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-H. Cui, T. Adachi, H. Oyamada, Y. Kamada, T. Kuwasaki, Y. Yamada, N. Saito, H. Kayaba, and J. Chihara The Role of Mitogen-Activated Protein Kinases in Eotaxin-Induced Cytokine Production from Bronchial Epithelial Cells Am. J. Respir. Cell Mol. Biol., September 1, 2002; 27(3): 329 - 335. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. E. L. Stubbs, P. Schratl, A. Hartnell, T. J. Williams, B. A. Peskar, A. Heinemann, and I. Sabroe Indomethacin Causes Prostaglandin D2-like and Eotaxin-like Selective Responses in Eosinophils and Basophils J. Biol. Chem., July 12, 2002; 277(29): 26012 - 26020. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Banas, M. Wornle, T. Berger, P. J. Nelson, C. D. Cohen, M. Kretzler, J. Pfirstinger, M. Mack, M. Lipp, H.-J. Grone, et al. Roles of SLC/CCL21 and CCR7 in Human Kidney for Mesangial Proliferation, Migration, Apoptosis, and Tissue Homeostasis J. Immunol., May 1, 2002; 168(9): 4301 - 4307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Bandeira-Melo, K. Sugiyama, L. J. Woods, M. Phoofolo, D. M. Center, W. W. Cruikshank, and P. F. Weller IL-16 Promotes Leukotriene C4 and IL-4 Release from Human Eosinophils via CD4- and Autocrine CCR3-Chemokine-Mediated Signaling J. Immunol., May 1, 2002; 168(9): 4756 - 4763. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. D'AMBROSIO, M. MARIANI, P. PANINA-BORDIGNON, and F. SINIGAGLIA Chemokines and Their Receptors Guiding T Lymphocyte Recruitment in Lung Inflammation Am. J. Respir. Crit. Care Med., October 1, 2001; 164(7): 1266 - 1275. [Full Text] [PDF]
|
|
|
|
|
|
C. Bandeira-Melo, A. Herbst, and P. F. Weller Eotaxins . Contributing to the Diversity of Eosinophil Recruitment and Activation Am. J. Respir. Cell Mol. Biol., June 1, 2001; 24(6): 653 - 657. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
