| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Unité d’Immuno-Allergie, Institut Pasteur, Paris, France;
Institut Jacques Monod, Unité Mixte de Recherche 7592, Paris, France; and
Laboratoire de Chimie Structurale des Macromolécules (Unité de Recherche Associeé 2185, Centre National de la Recherche Scientifique), Paris, France
Mitogenic activity of bone marrow-derived mouse mast cells and mast
cell lines P815 and MC/9 on B and T lymphocytes is present in their
culture supernatants. To identify this activity, mast cells were
incubated in serum-free medium and the supernatant was subjected to
differential centrifugation, which resulted in two fractions, the
hypodense and dense fraction (pellet). When analyzed for their
mitogenic activity on spleen cells, all activity was found to be
associated with the dense fraction. Electron microscopy studies
revealed the presence in this fraction of small vesicles called
exosomes with a heterogeneous size from 60 to 100 nm of diameter. When
cocultured with spleen cells, purified exosomes induced blast
formation, proliferation, as well as IL-2 and IFN-
production, but
no detectable IL-4. Similar data were obtained by injecting exosomes
into naive mice. In contrast to mast cell lines, a pretreatment with
IL-4 is required for bone marrow-derived mast cells to secrete active
exosomes. Structurally, exosomes were found to harbor immunologically
relevant molecules such as MHC class II, CD86, LFA-1, and ICAM-1. These
findings indicate that mast cells can represent a critical component of
the immunoregulatory network through secreted exosomes that display
mitogenic activity on B and T lymphocytes both in vitro and in
vivo.
This article has been cited by other articles:
|
|
 |
|
  M. Kesimer, M. Scull, B. Brighton, G. DeMaria, K. Burns, W. O'Neal, R. J. Pickles, and J. K. Sheehan Characterization of exosome-like vesicles released from human tracheobronchial ciliated epithelium: a possible role in innate defense FASEB J, June 1, 2009; 23(6): 1858 - 1868. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. Khatua, H. E. Taylor, J. E. K. Hildreth, and W. Popik Exosomes Packaging APOBEC3G Confer Human Immunodeficiency Virus Resistance to Recipient Cells J. Virol., January 15, 2009; 83(2): 512 - 521. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G.-J. Wang, Y. Liu, A. Qin, S. V. Shah, Z.-b. Deng, X. Xiang, Z. Cheng, C. Liu, J. Wang, L. Zhang, et al. Thymus Exosomes-Like Particles Induce Regulatory T Cells J. Immunol., October 15, 2008; 181(8): 5242 - 5248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Prado, E. G. Marazuela, E. Segura, H. Fernandez-Garcia, M. Villalba, C. Thery, R. Rodriguez, and E. Batanero Exosomes from Bronchoalveolar Fluid of Tolerized Mice Prevent Allergic Reaction J. Immunol., July 15, 2008; 181(2): 1519 - 1525. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Saunderson, P. C. Schuberth, A. C. Dunn, L. Miller, B. D. Hock, P. A. MacKay, N. Koch, R. W. Jack, and A. D. McLellan Induction of Exosome Release in Primary B Cells Stimulated via CD40 and the IL-4 Receptor J. Immunol., June 15, 2008; 180(12): 8146 - 8152. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. L. Sprague, B. D. Elzey, S. A. Crist, T. J. Waldschmidt, R. J. Jensen, and T. L. Ratliff Platelet-mediated modulation of adaptive immunity: unique delivery of CD154 signal by platelet-derived membrane vesicles Blood, May 15, 2008; 111(10): 5028 - 5036. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. J. C. Quah and H. C. O'Neill Mycoplasma contaminants present in exosome preparations induce polyclonal B cell responses J. Leukoc. Biol., November 1, 2007; 82(5): 1070 - 1082. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Clayton, J. P. Mitchell, J. Court, M. D. Mason, and Z. Tabi Human Tumor-Derived Exosomes Selectively Impair Lymphocyte Responses to Interleukin-2 Cancer Res., August 1, 2007; 67(15): 7458 - 7466. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. van Niel, I. Porto-Carreiro, S. Simoes, and G. Raposo Exosomes: a common pathway for a specialized function. J. Biochem., July 1, 2006; 140(1): 13 - 21. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kolegraff, P. Bostik, and A. A. Ansari Characterization and role of lentivirus-associated host proteins. Experimental Biology and Medicine, March 1, 2006; 231(3): 252 - 263. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Al-Nedawi, J. Szemraj, and C. S. Cierniewski Mast Cell-Derived Exosomes Activate Endothelial Cells to Secrete Plasminogen Activator Inhibitor Type 1 Arterioscler Thromb Vasc Biol, August 1, 2005; 25(8): 1744 - 1749. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-P. Caby, D. Lankar, C. Vincendeau-Scherrer, G. Raposo, and C. Bonnerot Exosomal-like vesicles are present in human blood plasma Int. Immunol., July 1, 2005; 17(7): 879 - 887. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-H. Kim, E. R. Lechman, N. Bianco, R. Menon, A. Keravala, J. Nash, Z. Mi, S. C. Watkins, A. Gambotto, and P. D. Robbins Exosomes Derived from IL-10-Treated Dendritic Cells Can Suppress Inflammation and Collagen-Induced Arthritis J. Immunol., May 15, 2005; 174(10): 6440 - 6448. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Roy, G. Martin, J.-F. Giguere, D. Belanger, M. Petrin, and M. J. Tremblay HIV Type 1 Can Act as an APC upon Acquisition from the Host Cell of Peptide-Loaded HLA-DR and CD86 Molecules J. Immunol., April 15, 2005; 174(8): 4779 - 4788. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Fevrier, D. Vilette, F. Archer, D. Loew, W. Faigle, M. Vidal, H. Laude, and G. Raposo Cells release prions in association with exosomes PNAS, June 29, 2004; 101(26): 9683 - 9688. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Andre, N. Chaput, N. E. C. Schartz, C. Flament, N. Aubert, J. Bernard, F. Lemonnier, G. Raposo, B. Escudier, D.-H. Hsu, et al. Exosomes as Potent Cell-Free Peptide-Based Vaccine. I. Dendritic Cell-Derived Exosomes Transfer Functional MHC Class I/Peptide Complexes to Dendritic Cells J. Immunol., February 15, 2004; 172(4): 2126 - 2136. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Admyre, J. Grunewald, J. Thyberg, S. Gripenback, G. Tornling, A. Eklund, A. Scheynius, and S. Gabrielsson Exosomes with major histocompatibility complex class II and co-stimulatory molecules are present in human BAL fluid Eur. Respir. J., October 1, 2003; 22(4): 578 - 583. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-K. Yu and C.-L. Chen Activation of Mast Cells Is Essential for Development of House Dust Mite Dermatophagoides farinae-Induced Allergic Airway Inflammation in Mice J. Immunol., October 1, 2003; 171(7): 3808 - 3815. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Gould, A. M. Booth, and J. E. K. Hildreth The Trojan exosome hypothesis PNAS, September 16, 2003; 100(19): 10592 - 10597. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Flanagan, J. Middeldorp, and T. Sculley Localization of the Epstein-Barr virus protein LMP 1 to exosomes J. Gen. Virol., July 1, 2003; 84(7): 1871 - 1879. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Wubbolts, R. S. Leckie, P. T. M. Veenhuizen, G. Schwarzmann, W. Mobius, J. Hoernschemeyer, J.-W. Slot, H. J. Geuze, and W. Stoorvogel Proteomic and Biochemical Analyses of Human B Cell-derived Exosomes. POTENTIAL IMPLICATIONS FOR THEIR FUNCTION AND MULTIVESICULAR BODY FORMATION J. Biol. Chem., March 21, 2003; 278(13): 10963 - 10972. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Skokos, H. G. Botros, C. Demeure, J. Morin, R. Peronet, G. Birkenmeier, S. Boudaly, and S. Mecheri Mast Cell-Derived Exosomes Induce Phenotypic and Functional Maturation of Dendritic Cells and Elicit Specific Immune Responses In Vivo J. Immunol., March 15, 2003; 170(6): 3037 - 3045. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. W. Wong, P. S. Foster, S. Yasuda, J. C. Qi, S. Mahalingam, E. A. Mellor, G. Katsoulotos, L. Li, J. A. Boyce, S. A. Krilis, et al. Biochemical and Functional Characterization of Human Transmembrane Tryptase (TMT)/Tryptase gamma . TMT IS AN EXOCYTOSED MAST CELL PROTEASE THAT INDUCES AIRWAY HYPERRESPONSIVENESS IN VIVO VIA AN INTERLEUKIN-13/INTERLEUKIN-4 RECEPTOR alpha /SIGNAL TRANSDUCER AND ACTIVATOR OF TRANSCRIPTION (STAT) 6-DEPENDENT PATHWAY J. Biol. Chem., October 25, 2002; 277(44): 41906 - 41915. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. Mellon, B. T. Frank, and K. C. Fang Mast Cell {alpha}-Chymase Reduces IgE Recognition of Birch Pollen Profilin by Cleaving Antibody-Binding Epitopes J. Immunol., January 1, 2002; 168(1): 290 - 297. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
