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Emory Vaccine Center and Department of Microbiology and Immunology, Emory University, Atlanta, GA 30332
Following infection with intracellular pathogens, Ag-specific
CD8+ T cells become activated and begin to proliferate. As
these cells become activated, they elaborate effector functions
including cytokine production and cytolysis. After the infection has
been cleared, the immune system returns to homeostasis through
apoptosis of the majority of the Ag-specific effector cells. The
surviving memory cells can persist for extended periods and provide
protection against reinfection. Little is known about the changes in
gene expression as Ag-specific cells progress through these stages of
development, i.e., naive to effector to memory. Using recombinant MHC
class I tetramers, we isolated Ag-specific CD8+ T cells
from mice infected with lymphocytic choriomeningitis virus at various
time points and performed semiquantitative RT-PCR. We examined
expression of: 1) genes involved in cell cycle control, 2) effector and
regulatory functions, and 3) susceptibility to apoptosis. We found that
Ag-specific CD8+ memory T cells contain high steady-state
levels of Bcl-2, Bax, IFN-
, and lung
Kruppel-like factor (LKLF), and decreased levels of p21
and p27 mRNA. Moreover, the pattern of gene expression between naive
and memory cells is distinct and suggests that these two cell types
control susceptibility to apoptosis through different
mechanisms.
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