The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zaslaver, A.
Right arrow Articles by Ben-Baruch, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zaslaver, A.
Right arrow Articles by Ben-Baruch, A.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH
Hazardous Substances DB
*CYTOCHALASIN D
The Journal of Immunology, 2001, 166: 1272-1284.
Copyright © 2001 by The American Association of Immunologists

Actin Filaments Are Involved in the Regulation of Trafficking of Two Closely Related Chemokine Receptors, CXCR1 and CXCR2

Alon Zaslaver, Rotem Feniger-Barish and Adit Ben-Baruch

Department of Cell Research and Immunology, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv, Israel

The ligand-induced internalization and recycling of chemokine receptors play a significant role in their regulation. In this study, we analyzed the involvement of actin filaments and of microtubules in the control of ligand-induced internalization and recycling of CXC chemokine receptor (CXCR)1 and CXCR2, two closely related G protein-coupled receptors that mediate ELR-expressing CXC chemokine-induced cellular responses. Nocodazole, a microtubule-disrupting agent, did not affect the IL-8-induced reduction in cell surface expression of CXCR1 and CXCR2, nor did it affect the recycling of these receptors following ligand removal and cell recovery at 37°C. In contrast, cytochalasin D, an actin filament depolymerizing agent, promoted the IL-8-induced reduction in cell surface expression of both CXCR1 and CXCR2. Cytochalasin D significantly inhibited the recycling of both CXCR1 and CXCR2 following IL-8-induced internalization, the inhibition being more pronounced for CXCR2 than for CXCR1. Potent inhibition of recycling was observed also when internalization of CXCR2 was induced by another ELR-expressing CXC chemokine, granulocyte chemotactic protein-2. By the use of carboxyl terminus-truncated CXCR1 and CXCR2 it was observed that the carboxyl terminus domains of CXCR1 and CXCR2 were partially involved in the regulation of the actin-mediated process of receptor recycling. The cytochalasin D-mediated inhibition of CXCR2 recycling had a functional relevance because it impaired the ability of CXCR2-expressing cells to mediate cellular responses. These results suggest that actin filaments, but not microtubules, are involved in the regulation of the intracellular trafficking of CXCR1 and CXCR2, and that actin filaments may be required to enable cellular resensitization following a desensitized refractory period.




This article has been cited by other articles:


Home page
 Rheumatology (Oxford)Home page
S.-C. Hsieh, T.-H. Wu, C.-Y. Tsai, K.-J. Li, M.-C. Lu, C.-H. Wu, and C.-L. Yu
Abnormal in vitro CXCR2 modulation and defective cationic ion transporter expression on polymorphonuclear neutrophils responsible for hyporesponsiveness to IL-8 stimulation in patients with active systemic lupus erythematosus
Rheumatology, February 1, 2008; 47(2): 150 - 157.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
P. T. Morrison, L. H. Thomas, M. Sharland, and J. S. Friedland
RSV-infected airway epithelial cells cause biphasic up-regulation of CCR1 expression on human monocytes
J. Leukoc. Biol., June 1, 2007; 81(6): 1487 - 1495.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
N. F. Neel, L. A. Lapierre, J. R. Goldenring, and A. Richmond
RhoB plays an essential role in CXCR2 sorting decisions
J. Cell Sci., May 1, 2007; 120(9): 1559 - 1571.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
D. Fu and B. D. Roufogalis
Actin disruption inhibits endosomal traffic of P-glycoprotein-EGFP and resistance to daunorubicin accumulation
Am J Physiol Cell Physiol, April 1, 2007; 292(4): C1543 - C1552.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. Dagan-Berger, R. Feniger-Barish, S. Avniel, H. Wald, E. Galun, V. Grabovsky, R. Alon, A. Nagler, A. Ben-Baruch, and A. Peled
Role of CXCR3 carboxyl terminus and third intracellular loop in receptor-mediated migration, adhesion and internalization in response to CXCL11
Blood, May 15, 2006; 107(10): 3821 - 3831.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Laroche, M. D. Rochdi, S. A. Laporte, and J.-L. Parent
Involvement of Actin in Agonist-induced Endocytosis of the G Protein-coupled Receptor for Thromboxane A2: OVERCOMING OF ACTIN DISRUPTION BY ARRESTIN-3 BUT NOT ARRESTIN-2
J. Biol. Chem., June 17, 2005; 280(24): 23215 - 23224.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
Q. Yan, W. Sun, P. Kujala, Y. Lotfi, T. A. Vida, and A. J. Bean
CART: An Hrs/Actinin-4/BERP/Myosin V Protein Complex Required for Efficient Receptor Recycling
Mol. Biol. Cell, May 1, 2005; 16(5): 2470 - 2482.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. J. Baron, G. R. Bolduc, M. B. Goldberg, T. C. Auperin, and L. C. Madoff
Alpha C Protein of Group B Streptococcus Binds Host Cell Surface Glycosaminoglycan and Enters Cells by an Actin-dependent Mechanism
J. Biol. Chem., June 4, 2004; 279(23): 24714 - 24723.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
G.-H. Fan, L. A. Lapierre, J. R. Goldenring, J. Sai, and A. Richmond
Rab11-Family Interacting Protein 2 and Myosin Vb Are Required for CXCR2 Recycling and Receptor-mediated Chemotaxis
Mol. Biol. Cell, May 1, 2004; 15(5): 2456 - 2469.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
S. A. Dunn, M. Mohteshamzadeh, A. K. Daly, and T. H. Thomas
Altered Tropomyosin Expression in Essential Hypertension
Hypertension, February 1, 2003; 41(2): 347 - 354.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. Roland, B. J. Murphy, B. Ahr, V. Robert-Hebmann, V. Delauzun, K. E. Nye, C. Devaux, and M. Biard-Piechaczyk
Role of the intracellular domains of CXCR4 in SDF-1-mediated signaling
Blood, January 15, 2003; 101(2): 399 - 406.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
G. Cheng, Y. Iijima, Y. Ishibashi, D. Kuppuswamy, and G. Cooper IV
Inhibition of G protein-coupled receptor trafficking in neuroblastoma cells by MAP 4 decoration of microtubules
Am J Physiol Heart Circ Physiol, December 1, 2002; 283(6): H2379 - H2388.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
T. Doroshenko, Y. Chaly, V. Savitskiy, O. Maslakova, A. Portyanko, I. Gorudko, and N. N. Voitenok
Phagocytosing neutrophils down-regulate the expression of chemokine receptors CXCR1 and CXCR2
Blood, September 18, 2002; 100(7): 2668 - 2671.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Geminder, O. Sagi-Assif, L. Goldberg, T. Meshel, G. Rechavi, I. P. Witz, and A. Ben-Baruch
A Possible Role for CXCR4 and Its Ligand, the CXC Chemokine Stromal Cell-Derived Factor-1, in the Development of Bone Marrow Metastases in Neuroblastoma
J. Immunol., October 15, 2001; 167(8): 4747 - 4757.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2001 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2001 by The American Association of Immunologists, Inc. All rights reserved.