| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Internal Medicine, Division of Pulmonary and Critical Care Medicine, University of Michigan Medical School, Ann Arbor, MI 48109
Tumors are dependent on angiogenesis for survival and propagation. Accumulated evidence suggests that macrophages are a potentially important source of angiogenic factors in many disease states. However, the role(s) of macrophages in non-small cell lung cancer (NSCLC) have not been determined. We hypothesized that monocyte-derived macrophages are induced by NSCLC to increase expression of angiogenic factors. To define the role of macrophage-tumor cell interaction with respect to angiogenesis, human peripheral blood monocytes (PBM) were cocultured with A549 (human bronchoalveolar cell carcinoma) or Calu 6 (human anaplastic carcinoma) NSCLC cells. The resultant conditioned medium (CM) was evaluated for angiogenic potential and for expression of angiogenic factors. We found that endothelial cell chemotactic activity (as a measure of angiogenic potential) was significantly increased in response to CM from cocultures of PBM/NSCLC compared with PBM alone, NSCLC alone, or a combination of NSCLC and PBM CM generated separately. Subsequent analysis by ELISA reveals markedly increased CXC chemokine expression, with a lesser increase in vascular endothelial growth factor, in CM from PBM/NSCLC coculture. Neutralizing Ab to angiogenic CXC chemokines blocked the increase in endothelial cell chemotaxis. Furthermore, with separately generated CM as a stimulus, we found that macrophages are the predominant source of increased CXC chemokine expression. Finally, we found that NSCLC-derived macrophage migration-inhibitory factor is responsible for the increased expression of macrophage-derived angiogenic activity. These data suggest that the interaction between host macrophages and NSCLC cells synergistically increases angiogenic potential, and that this is due to an increased elaboration of angiogenic CXC chemokines.
This article has been cited by other articles:
|
|
 |
|
  Q. Zhou, X. Yan, J. Gershan, R. J. Orentas, and B. D. Johnson Expression of Macrophage Migration Inhibitory Factor by Neuroblastoma Leads to the Inhibition of Antitumor T Cell Reactivity In Vivo J. Immunol., August 1, 2008; 181(3): 1877 - 1886. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. E. Rendon, T. Roger, I. Teneng, M. Zhao, Y. Al-Abed, T. Calandra, and R. A. Mitchell Regulation of Human Lung Adenocarcinoma Cell Migration and Invasion by Macrophage Migration Inhibitory Factor J. Biol. Chem., October 12, 2007; 282(41): 29910 - 29918. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. McClelland, S. L. Carskadon, L. Zhao, E. S. White, D. G. Beer, M. B. Orringer, A. Pickens, A. C. Chang, and D. A. Arenberg Diversity of the Angiogenic Phenotype in Non-Small Cell Lung Cancer Am. J. Respir. Cell Mol. Biol., March 1, 2007; 36(3): 343 - 350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Wald, U. Izhar, G. Amir, S. Avniel, Y. Bar-Shavit, H. Wald, I. D. Weiss, E. Galun, and A. Peled CD4+CXCR4highCD69+ T Cells Accumulate in Lung Adenocarcinoma J. Immunol., November 15, 2006; 177(10): 6983 - 6990. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Swant, B. E. Rendon, M. Symons, and R. A. Mitchell Rho GTPase-dependent Signaling Is Required for Macrophage Migration Inhibitory Factor-mediated Expression of Cyclin D1 J. Biol. Chem., June 17, 2005; 280(24): 23066 - 23072. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.-L. Yao, Y.-C. Lin, C.-H. Wang, Y.-C. Huang, W.-Y. Liao, S.-S. Wang, J. J. W. Chen, and P.-C. Yang Autocrine and Paracrine Regulation of Interleukin-8 Expression in Lung Cancer Cells Am. J. Respir. Cell Mol. Biol., June 1, 2005; 32(6): 540 - 547. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J.W. Chen, Y.-C. Lin, P.-L. Yao, A. Yuan, H.-Y. Chen, C.-T. Shun, M.-F. Tsai, C.-H. Chen, and P.-C. Yang Tumor-Associated Macrophages: The Double-Edged Sword in Cancer Progression J. Clin. Oncol., February 10, 2005; 23(5): 953 - 964. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. Langenfeld and J. Langenfeld Bone Morphogenetic Protein-2 Stimulates Angiogenesis in Developing Tumors Mol. Cancer Res., March 1, 2004; 2(3): 141 - 149. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Campa, S. Zienolddiny, V. Maggini, V. Skaug, A. Haugen, and F. Canzian Association of a common polymorphism in the cyclooxygenase 2 gene with risk of non-small cell lung cancer Carcinogenesis, February 1, 2004; 25(2): 229 - 235. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Amin, O. V. Volpert, J. M. Woods, P. Kumar, L. A. Harlow, and A. E. Koch Migration Inhibitory Factor Mediates Angiogenesis via Mitogen-Activated Protein Kinase and Phosphatidylinositol Kinase Circ. Res., August 22, 2003; 93(4): 321 - 329. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J.W. Chen, P.-L. Yao, A. Yuan, T.-M. Hong, C.-T. Shun, M.-L. Kuo, Y.-C. Lee, and P.-C. Yang Up-Regulation of Tumor Interleukin-8 Expression by Infiltrating Macrophages: Its Correlation with Tumor Angiogenesis and Patient Survival in Non-Small Cell Lung Cancer Clin. Cancer Res., February 1, 2003; 9(2): 729 - 737. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. S. White, K. R. Flaherty, S. Carskadon, A. Brant, M. D. Iannettoni, J. Yee, M. B. Orringer, and D. A. Arenberg Macrophage Migration Inhibitory Factor and CXC Chemokine Expression in Non-Small Cell Lung Cancer: Role in Angiogenesis and Prognosis Clin. Cancer Res., February 1, 2003; 9(2): 853 - 860. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Tomiyasu, I. Yoshino, R. Suemitsu, T. Okamoto, and K. Sugimachi Quantification of Macrophage Migration Inhibitory Factor mRNA Expression in Non-Small Cell Lung Cancer Tissues and Its Clinical Significance Clin. Cancer Res., December 1, 2002; 8(12): 3755 - 3760. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Saijo, M. Tanaka, M. Miki, K. Usui, T. Suzuki, M. Maemondo, X. Hong, R. Tazawa, T. Kikuchi, K. Matsushima, et al. Proinflammatory Cytokine IL-1{beta} Promotes Tumor Growth of Lewis Lung Carcinoma by Induction of Angiogenic Factors: In Vivo Analysis of Tumor-Stromal Interaction J. Immunol., July 1, 2002; 169(1): 469 - 475. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Barchowsky, N. V. Soucy, K. A. O'Hara, J. Hwa, T. L. Noreault, and A. S. Andrew A Novel Pathway for Nickel-induced Interleukin-8 Expression J. Biol. Chem., June 28, 2002; 277(27): 24225 - 24231. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. S. White, D. L. Livant, S. Markwart, and D. A. Arenberg Monocyte-Fibronectin Interactions, Via {alpha}5{beta}1 Integrin, Induce Expression of CXC Chemokine-Dependent Angiogenic Activity J. Immunol., November 1, 2001; 167(9): 5362 - 5366. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
