| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Veterinary Biosciences, Ohio State University, Columbus, OH 43210
Gastritis due to Helicobacter pylori in mice and
humans is considered a Th1-mediated disease, but the specific cell
subsets and cytokines involved are still not well understood. The goal
of this study was to investigate the immunopathogenesis of H.
pylori-induced gastritis and delayed-type hypersensitivity
(DTH) in mice. C57BL/6-Prkdcscid mice were
infected with H. pylori and reconstituted with
CD4+, CD4-depleted, CD4+CD45RBhigh,
or CD4+CD45RBlow splenocytes from wild-type
C57BL/6 mice or with splenocytes from
C57BL/6IFN-
-/- or
C57BL/6IL-10-/- mice. Four or eight weeks after transfer,
DTH to H. pylori Ags was determined by footpad
injection; gastritis and bacterial colonization were quantified; and
IFN- secretion by splenocytes in response to H.
pylori Ag was determined. Gastritis and DTH were present in
recipients of unfractionated splenocytes, CD4+ splenocytes,
and CD4+CD45RBhigh splenocytes, but absent in
the other groups. IFN- secretion in response to H.
pylori Ags was correlated with gastritis, although splenocytes
from all groups of mice secreted some IFN-. Gastritis was most
severe in recipients of splenocytes from IL-10-deficient mice, and
least severe in those given IFN--deficient splenocytes. Bacterial
colonization in all groups was inversely correlated with gastritis.
These data indicate that 1) CD4+ T cells are both necessary
and sufficient for gastritis and DTH due to H. pylori in
mice; 2) high expression of CD45RB is a marker for gastritis-inducing
CD4+ cells; and 3) IFN- contributes to gastritis and
IL-10 suppresses it, but IFN- secretion alone is not sufficient to
induce gastritis. The results support the assertion that H.
pylori is mediated by a Th1-biased cellular immune
response.
This article has been cited by other articles:
|
|
 |
|
  K.-P. Ko, S. K. Park, L. Y. Cho, J. Gwack, J. J. Yang, A. Shin, C. S. Kim, Y. Kim, D. Kang, S.-H. Chang, et al. Soybean Product Intake Modifies the Association between Interleukin-10 Genetic Polymorphisms and Gastric Cancer Risk J. Nutr., May 1, 2009; 139(5): 1008 - 1012. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. L.W. Wong, S.-L. Zhu, X. R. Huang, J. Ma, H. H.X. Xia, R. Bucala, B. C.Y. Wong, and H. Y. Lan Essential Role for Macrophage Migration Inhibitory Factor in Gastritis Induced by Helicobacter pylori Am. J. Pathol., April 1, 2009; 174(4): 1319 - 1328. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. P. Tan, J. Pedersen, Y. Zhan, A. M. Lew, M. J. Pearse, O. L. C. Wijburg, and R. A. Strugnell CD8+ T Cells Are Associated with Severe Gastritis in Helicobacter pylori- Infected Mice in the Absence of CD4+ T Cells Infect. Immun., March 1, 2008; 76(3): 1289 - 1297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-W. Lee, V. P. Rao, A. B. Rogers, Z. Ge, S. E. Erdman, M. T. Whary, and J. G. Fox Wild-Type and Interleukin-10-Deficient Regulatory T Cells Reduce Effector T-Cell-Mediated Gastroduodenitis in Rag2-/- Mice, but Only Wild-Type Regulatory T Cells Suppress Helicobacter pylori Gastritis Infect. Immun., June 1, 2007; 75(6): 2699 - 2707. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Caruso, D. Fina, I. Peluso, M. C. Fantini, C. Tosti, G. D. V. Blanco, O. A. Paoluzi, F. Caprioli, F. Andrei, C. Stolfi, et al. IL-21 Is Highly Produced in Helicobacter pylori-Infected Gastric Mucosa and Promotes Gelatinases Synthesis J. Immunol., May 1, 2007; 178(9): 5957 - 5965. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kiriya, N. Watanabe, A. Nishio, K. Okazaki, M. Kido, K. Saga, J. Tanaka, T. Akamatsu, S. Ohashi, M. Asada, et al. Essential role of Peyer's patches in the development of Helicobacter-induced gastritis Int. Immunol., April 1, 2007; 19(4): 435 - 446. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. M. S. Algood and T. L. Cover Helicobacter pylori Persistence: an Overview of Interactions between H. pylori and Host Immune Defenses Clin. Microbiol. Rev., October 1, 2006; 19(4): 597 - 613. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Eaton, L. H. Benson, J. Haeger, and B. M. Gray Role of Transcription Factor T-bet Expression by CD4+ Cells in Gastritis Due to Helicobacter pylori in Mice. Infect. Immun., August 1, 2006; 74(8): 4673 - 4684. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. Kusters, A. H. M. van Vliet, and E. J. Kuipers Pathogenesis of Helicobacter pylori Infection Clin. Microbiol. Rev., July 1, 2006; 19(3): 449 - 490. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Y. Kao, S. Rathinavelu, K. A. Eaton, L. Bai, Y. Zavros, M. Takami, A. Pierzchala, and J. L. Merchant Helicobacter pylori-secreted factors inhibit dendritic cell IL-12 secretion: a mechanism of ineffective host defense Am J Physiol Gastrointest Liver Physiol, July 1, 2006; 291(1): G73 - G81. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kaparakis, K. L. Laurie, O. Wijburg, J. Pedersen, M. Pearse, I. R. van Driel, P. A. Gleeson, and R. A. Strugnell CD4+ CD25+ Regulatory T Cells Modulate the T-Cell and Antibody Responses in Helicobacter-Infected BALB/c Mice. Infect. Immun., June 1, 2006; 74(6): 3519 - 3529. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Anderson, S. J. Czinn, R. W. Redline, and T. G. Blanchard Induction of CTLA-4-Mediated Anergy Contributes to Persistent Colonization in the Murine Model of Gastric Helicobacter pylori Infection J. Immunol., May 1, 2006; 176(9): 5306 - 5313. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Tan, C. D. Fraley, M. Zhang, D. Dailidiene, A. Kornberg, and D. E. Berg Diverse Phenotypes Resulting from Polyphosphate Kinase Gene (ppk1) Inactivation in Different Strains of Helicobacter pylori J. Bacteriol., November 15, 2005; 187(22): 7687 - 7695. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Lundgren, C. Trollmo, A. Edebo, A.-M. Svennerholm, and B. S. Lundin Helicobacter pylori-Specific CD4+ T Cells Home to and Accumulate in the Human Helicobacter pylori-Infected Gastric Mucosa Infect. Immun., September 1, 2005; 73(9): 5612 - 5619. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yamaoka, K. Yamauchi, H. Ota, A. Sugiyama, S. Ishizone, D. Y. Graham, F. Maruta, M. Murakami, and T. Katsuyama Natural History of Gastric Mucosal Cytokine Expression in Helicobacter pylori Gastritis in Mongolian Gerbils Infect. Immun., April 1, 2005; 73(4): 2205 - 2212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Sebkova, A. Pellicano, G. Monteleone, B. Grazioli, G. Guarnieri, M. Imeneo, F. Pallone, and F. Luzza Extracellular Signal-Regulated Protein Kinase Mediates Interleukin 17 (IL-17)-Induced IL-8 Secretion in Helicobacter pylori-Infected Human Gastric Epithelial Cells Infect. Immun., September 1, 2004; 72(9): 5019 - 5026. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R Rad, A Dossumbekova, B Neu, R Lang, S Bauer, D Saur, M Gerhard, and C Prinz Cytokine gene polymorphisms influence mucosal cytokine expression, gastric inflammation, and host specific colonisation during Helicobacter pylori infection Gut, August 1, 2004; 53(8): 1082 - 1089. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Eaton, S. M. Logan, P. E. Baker, R. A. Peterson, M. A. Monteiro, and E. Altman Helicobacter pylori with a Truncated Lipopolysaccharide O Chain Fails To Induce Gastritis in SCID Mice Injected with Splenocytes from Wild-Type C57BL/6J Mice Infect. Immun., July 1, 2004; 72(7): 3925 - 3931. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zavros, J. Y. Kao, and J. L. Merchant Inflammation and Cancer III. Somatostatin and the innate immune system Am J Physiol Gastrointest Liver Physiol, May 1, 2004; 286(5): G698 - G701. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Akhiani, K. Schon, L. E. Franzen, J. Pappo, and N. Lycke Helicobacter pylori-Specific Antibodies Impair the Development of Gastritis, Facilitate Bacterial Colonization, and Counteract Resistance against Infection J. Immunol., April 15, 2004; 172(8): 5024 - 5033. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Macarthur, G. L. Hold, and E. M. El-Omar Inflammation and Cancer II. Role of chronic inflammation and cytokine gene polymorphisms in the pathogenesis of gastrointestinal malignancy Am J Physiol Gastrointest Liver Physiol, April 1, 2004; 286(4): G515 - G520. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. F. Ismail, J. Zhang, R. G. Lynch, Y. Wang, and D. J. Berg Role for Complement in Development of Helicobacter-Induced Gastritis in Interleukin-10-Deficient Mice Infect. Immun., December 1, 2003; 71(12): 7140 - 7148. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Zavros, S. Rathinavelu, J. Y. Kao, A. Todisco, J. Del Valle, J. V. Weinstock, M. J. Low, and J. L. Merchant Treatment of Helicobacter gastritis with IL-4 requires somatostatin PNAS, October 28, 2003; 100(22): 12944 - 12949. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Meyer, K. S. Ramanujam, A. P. Gobert, S. P. James, and K. T. Wilson Cutting Edge: Cyclooxygenase-2 Activation Suppresses Th1 Polarization in Response to Helicobacter pylori J. Immunol., October 15, 2003; 171(8): 3913 - 3917. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S Futagami, T Hiratsuka, A Tatsuguchi, K Suzuki, M Kusunoki, Y Shinji, K Shinoki, T Iizumi, T Akamatsu, H Nishigaki, et al. Monocyte chemoattractant protein 1 (MCP-1) released from Helicobacter pylori stimulated gastric epithelial cells induces cyclooxygenase 2 expression and activation in T cells Gut, September 1, 2003; 52(9): 1257 - 1264. [Abstract] [Full Text]
|
|
|
|
|
|
H. F. Ismail, P. Fick, J. Zhang, R. G. Lynch, and D. J. Berg Depletion of Neutrophils in IL-10-/- Mice Delays Clearance of Gastric Helicobacter Infection and Decreases the Th1 Immune Response to Helicobacter J. Immunol., April 1, 2003; 170(7): 3782 - 3789. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Syder, J. D. Oh, J. L. Guruge, D. O'Donnell, M. Karlsson, J. C. Mills, B. M. Bjorkholm, and J. I. Gordon The impact of parietal cells on Helicobacter pylori tropism and host pathology: An analysis using gnotobiotic normal and transgenic mice PNAS, March 18, 2003; 100(6): 3467 - 3472. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Krauss-Etschmann, E. Sammler, S. Koletzko, N. Konstantopoulos, D. Aust, B. Gebert, B. Luckow, D. Reinhardt, and D. J. Schendel Chemokine Receptor 5 Expression in Gastric Mucosa of Helicobacter pylori-Infected and Noninfected Children Clin. Vaccine Immunol., January 1, 2003; 10(1): 22 - 29. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Akhiani, J. Pappo, Z. Kabok, K. Schon, W. Gao, L. E. Franzen, and N. Lycke Protection Against Helicobacter pylori Infection Following Immunization Is IL-12-Dependent and Mediated by Th1 Cells J. Immunol., December 15, 2002; 169(12): 6977 - 6984. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
