| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Section of Atherosclerosis, Department of Medicine,
Center for Comparative Medicine,
Speros P. Martel Laboratory of Leukocyte Biology, Department of Pediatrics, and
Section of Infectious Disease, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030
Mice deficient in CD18, which lack all four CD11 integrins, have leukocytosis and increased susceptibility to bacterial infection. To determine the effect of deficiencies in LFA-1 (CD11a/CD18) or Mac-1 (CD11b/CD18) on host defense against systemic bacterial infection, knockout mice were inoculated i.p. with Streptococcus pneumoniae. Increased mortality occurred in both LFA-1-/- (15 of 17 vs 13 of 35 in wild type (WT), p < 0.01) and Mac-1-/- (17 of 34 vs 6 of 25, p < 0.01) mice. All deaths in LFA-1-/- mice occurred after 72 h, whereas most deaths in Mac-1-/- mice occurred within 24–48 h. At 24 h, 21 of 27 Mac-1-/- mice were bacteremic, vs 15 of 25 WT (p = 0.05); no difference was observed between LFA-1-/- and WT. Increased bacteria were recovered from Mac-1-/- spleens at 2 h (p = 0.03) and 6 h (p = 0.002) and from livers (p = 0.001) by 6 h. No difference was observed at 2 h in LFA-1-/- mice, but by 6 h increased bacteria were recovered from spleens (p = 0.008) and livers (p = 0.04). Baseline and peak leukocyte counts were similar between Mac-1-/- and WT, but elevated in LFA-1-/-. At 8 h, peritoneal neutrophils were increased in Mac-1-/-, but not significantly different in LFA-1-/-. Histopathologically, at 24 h Mac-1-/- animals had bacteremia and lymphoid depletion, consistent with sepsis. LFA-1-/- mice had increased incidence of otitis media and meningitis/encephalitis vs WT at 72 and 96 h. Both Mac-1 and LFA-1 play important but distinct roles in host defense to S. pneumoniae.
This article has been cited by other articles:
|
|
 |
|
  M. Gil, F. X. McCormack, and A. M. LeVine Surfactant Protein A Modulates Cell Surface Expression of CR3 on Alveolar Macrophages and Enhances CR3-mediated Phagocytosis J. Biol. Chem., March 20, 2009; 284(12): 7495 - 7504. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. U. Eisenhardt, M. Schwarz, N. Schallner, J. Soosairajah, N. Bassler, D. Huang, C. Bode, and K. Peter Generation of activation-specific human anti-{alpha}M{beta}2 single-chain antibodies as potential diagnostic tools and therapeutic agents Blood, April 15, 2007; 109(8): 3521 - 3528. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Li, A. R. Burns, and C. W. Smith Lymphocyte Function-Associated Antigen-1-Dependent Inhibition of Corneal Wound Healing Am. J. Pathol., November 1, 2006; 169(5): 1590 - 1600. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ghosh, A. A. Chackerian, C. M. Parker, C. M. Ballantyne, and S. M. Behar The LFA-1 adhesion molecule is required for protective immunity during pulmonary Mycobacterium tuberculosis infection. J. Immunol., April 15, 2006; 176(8): 4914 - 4922. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Lowell Beautiful blistering Blood, February 1, 2006; 107(3): 849 - 850. [Full Text] [PDF]
|
|
|
|
|
|
W. Jia, H. Li, and Y.-W. He The extracellular matrix protein mindin serves as an integrin ligand and is critical for inflammatory cell recruitment Blood, December 1, 2005; 106(12): 3854 - 3859. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Thornton and L. S. McDaniel THP-1 Monocytes Up-Regulate Intercellular Adhesion Molecule 1 in Response to Pneumolysin from Streptococcus pneumoniae Infect. Immun., October 1, 2005; 73(10): 6493 - 6498. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Watts, F. J. M. Beurskens, I. Martin-Padura, C. M. Ballantyne, L. B. Klickstein, M. B. Brenner, and D. M. Lee Manifestations of Inflammatory Arthritis Are Critically Dependent on LFA-1 J. Immunol., March 15, 2005; 174(6): 3668 - 3675. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. T. Brandhorst, M. Wuthrich, B. Finkel-Jimenez, T. Warner, and B. S. Klein Exploiting Type 3 Complement Receptor for TNF-{alpha} Suppression, Immune Evasion, and Progressive Pulmonary Fungal Infection J. Immunol., December 15, 2004; 173(12): 7444 - 7453. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Ren, M. A. McCrory, C. Pass, D. C. Bullard, C. M. Ballantyne, Y. Xu, D. E. Briles, and A. J. Szalai The Virulence Function of Streptococcus pneumoniae Surface Protein A Involves Inhibition of Complement Activation and Impairment of Complement Receptor-Mediated Protection J. Immunol., December 15, 2004; 173(12): 7506 - 7512. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kandula and C. Abraham LFA-1 on CD4+ T Cells Is Required for Optimal Antigen-Dependent Activation In Vivo J. Immunol., October 1, 2004; 173(7): 4443 - 4451. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Wu, J. R. Rodgers, X.-Y. D. Perrard, J. L. Perrard, J. E. Prince, Y. Abe, B. K. Davis, G. Dietsch, C. W. Smith, and C. M. Ballantyne Deficiency of CD11b or CD11d Results in Reduced Staphylococcal Enterotoxin-Induced T Cell Response and T Cell Phenotypic Changes J. Immunol., July 1, 2004; 173(1): 297 - 306. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Wu, J. E. Prince, C. F. Brayton, C. Shah, D. Zeve, S. H. Gregory, C. W. Smith, and C. M. Ballantyne Host Resistance of CD18 Knockout Mice against Systemic Infection with Listeria monocytogenes Infect. Immun., October 1, 2003; 71(10): 5986 - 5993. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Schramm, T. Schaefer, M. D. Menger, and H. Thorlacius Acute mast cell-dependent neutrophil recruitment in the skin is mediated by KC and LFA-1: inhibitory mechanisms of dexamethasone J. Leukoc. Biol., December 1, 2002; 72(6): 1122 - 1132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kadono, G. M. Venturi, D. A. Steeber, and T. F. Tedder Leukocyte Rolling Velocities and Migration Are Optimized by Cooperative L-Selectin and Intercellular Adhesion Molecule-1 Functions J. Immunol., October 15, 2002; 169(8): 4542 - 4550. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
