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to Bad1
*
Department of Immunology and Oncology, Centro Nacional de Biotecnología, Campus de Cantoblanco, Madrid, Spain;
Laboratoire de Physiologie de la Reproduction, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Paris, France; and
Département d’Immunologie, Laboratoire de Signalisation Immunoparasitaire, Institut Pasteur, Paris, France
The diverse forms of protein phosphatase 1 (PP1) in vivo result
from the association of the catalytic subunit with different regulatory
subunits. We recently have described that PP1
is a Ras-activated Bad
phosphatase that regulates IL-2 deprivation-induced apoptosis. With the
yeast two-hybrid system, GST fusion proteins, indirect
immunofluorescence, and coimmunoprecipitation, we found that Bcl-2
interacts with PP1 and Bad. In contrast, Bad did not interact with
14-3-3 protein. Bcl-2 depletion decreased phosphatase activity and
association of PP1 to Bad. Bcl-2 contains the RIVAF motif, analogous
to the well characterized R/KXV/IXF consensus motif shared by most
PP1-interacting proteins. This sequence is involved in the binding of
Bcl-2 to PP1. Disruption of Bcl-2/PP1 association strongly
decreased Bcl-2 and Bad-associated phosphatase activity and formation
of the trimolecular complex. These results suggest that Bcl-2 targets
PP1 to Bad.
This article has been cited by other articles:
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  H.-S. Huang and E. Y. C. Lee Protein Phosphatase-1 Inhibitor-3 Is an in Vivo Target of Caspase-3 and Participates in the Apoptotic Response J. Biol. Chem., June 27, 2008; 283(26): 18135 - 18146. [Abstract] [Full Text] [PDF]
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 H. CEULEMANS and M. BOLLEN Functional Diversity of Protein Phosphatase-1, a Cellular Economizer and Reset Button Physiol Rev, January 1, 2004; 84(1): 1 - 39. [Abstract] [Full Text] [PDF]
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 R. C. Gupta, S. Mishra, S. Rastogi, M. Imai, O. Habib, and H. N. Sabbah Cardiac SR-coupled PP1 activity and expression are increased and inhibitor 1 protein expression is decreased in failing hearts Am J Physiol Heart Circ Physiol, December 1, 2003; 285(6): H2373 - H2381. [Abstract] [Full Text] [PDF]
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 W. Xu, X. Yuan, Y. J. Jung, Y. Yang, A. Basso, N. Rosen, E. J. Chung, J. Trepel, and L. Neckers The Heat Shock Protein 90 Inhibitor Geldanamycin and the ErbB Inhibitor ZD1839 Promote Rapid PP1 Phosphatase-Dependent Inactivation of AKT in ErbB2 Overexpressing Breast Cancer Cells Cancer Res., November 15, 2003; 63(22): 7777 - 7784. [Abstract] [Full Text] [PDF]
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P. Wakula, M. Beullens, H. Ceulemans, W. Stalmans, and M. Bollen Degeneracy and Function of the Ubiquitous RVXF Motif That Mediates Binding to Protein Phosphatase-1 J. Biol. Chem., May 23, 2003; 278(21): 18817 - 18823. [Abstract] [Full Text] [PDF]
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 V. Ayllon, A. Fleischer, X. Cayla, A. Garcia, and A. Rebollo Segregation of Bad from Lipid Rafts Is Implicated in the Induction of Apoptosis J. Immunol., April 1, 2002; 168(7): 3387 - 3393. [Abstract] [Full Text] [PDF]
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 P. T. W. Cohen Protein phosphatase 1 - targeted in many directions J. Cell Sci., January 15, 2002; 115(2): 241 - 256. [Abstract] [Full Text] [PDF]
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