| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Laboratory of Immunology, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224;
Mathematical and Statistical Computing Laboratory, Center of Information Technology, National Institutes of Health, Bethesda, MD 20892; and
DNA Array Unit, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224
In an attempt to understand the molecular basis for the
immunological memory response, we have used cDNA microarrays to measure
gene expression of human memory and naive CD4+ T cells at
rest and after activation. Our analysis of 54,768 cDNA clones provides
the first glimpse into gene expression patterns of memory and naive
CD4+ T cells at the genome-scale and reveals several novel
findings. First, memory and naive CD4+ T cells expressed
similar numbers of genes at rest and after activation. Second, we have
identified 14 cDNA clones that expressed higher levels of transcripts
in memory cells than in naive cells. Third, we have identified 135 (130
known genes and 5 expressed sequence tags) up-regulated and 68 (42
known genes and 26 expressed sequence tags) down-regulated cDNA clones
in memory CD4+ T after in vitro stimulation with
anti-CD3 plus anti-CD28. Interestingly, the increase in mRNA
levels of up-regulated genes was greater in memory than in naive
CD4+ T cells after in vitro stimulation and was higher with
anti-CD3 plus anti-CD28 than with anti-CD3 alone in both
memory and naive CD4+ T cells. Finally, the changes in
expression of actin and cytokine genes identified by cDNA microarrays
were confirmed by Northern and protein analyses. Together, we have
identified 
200 cDNA clones whose expression levels changed after
activation and suggest that the level of expression of up-regulated
genes is a molecular mechanism that differentiates the response of
memory from naive CD4+ T cells.
This article has been cited by other articles:
|
|
 |
|
  Y. Yang, J. An, and N.-p. Weng Telomerase Is Involved in IL-7-Mediated Differential Survival of Naive and Memory CD4+ T Cells J. Immunol., March 15, 2008; 180(6): 3775 - 3781. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. K. McKinstry, S. Golech, W.-H. Lee, G. Huston, N.-P. Weng, and S. L. Swain Rapid default transition of CD4 T cell effectors to functional memory cells J. Exp. Med., September 3, 2007; 204(9): 2199 - 2211. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Fann, J. M. Godlove, M. Catalfamo, W. H. Wood III, F. J. Chrest, N. Chun, L. Granger, R. Wersto, K. Madara, K. Becker, et al. Histone acetylation is associated with differential gene expression in the rapid and robust memory CD8+ T-cell response Blood, November 15, 2006; 108(10): 3363 - 3370. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bosco, K. L. McKenna, C. J. Devitt, M. J. Firth, P. D. Sly, and P. G. Holt Identification of novel th2-associated genes in T memory responses to allergens. J. Immunol., April 15, 2006; 176(8): 4766 - 4777. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Goda, T. Kanaji, S. Kanaji, G. Tanaka, K. Arima, S. Ohno, and K. Izuhara Involvement of IL-32 in activation-induced cell death in T cells Int. Immunol., February 1, 2006; 18(2): 233 - 240. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Li, W. Zhi, P. Wareski, and N.-p. Weng IL-15 Activates Telomerase and Minimizes Telomere Loss and May Preserve the Replicative Life Span of Memory CD8+ T Cells In Vitro J. Immunol., April 1, 2005; 174(7): 4019 - 4024. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Hess, Y. Yang, S. Golech, A. Sharov, K. G. Becker, and N.-p. Weng Kinetic assessment of general gene expression changes during human naive CD4+ T cell activation Int. Immunol., December 1, 2004; 16(12): 1711 - 1721. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Liu and S. I. Abrams Alterations in Fas Expression Are Characteristic of, But Not Solely Responsible for, Enhanced Metastatic Competence J. Immunol., June 15, 2003; 170(12): 5973 - 5980. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Liu and S. I. Abrams Coordinate Regulation of IFN Consensus Sequence-Binding Protein and Caspase-1 in the Sensitization of Human Colon Carcinoma Cells to Fas-Mediated Apoptosis by IFN-{gamma} J. Immunol., June 15, 2003; 170(12): 6329 - 6337. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Palena, J. Schlom, and K.-Y. Tsang Differential Gene Expression Profiles in a Human T-cell Line Stimulated with a Tumor-associated Self-peptide versus an Enhancer Agonist Peptide Clin. Cancer Res., May 1, 2003; 9(5): 1616 - 1627. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. D. Dixit, R. Sridaran, M. A. Edmonsond, D. Taub, and W. E. Thompson Gonadotropin-Releasing Hormone Attenuates Pregnancy-Associated Thymic Involution and Modulates the Expression of Antiproliferative Gene Product Prohibitin Endocrinology, April 1, 2003; 144(4): 1496 - 1505. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Manel, S. Kinet, J.-L. Battini, F. J. Kim, N. Taylor, and M. Sitbon The HTLV receptor is an early T-cell activation marker whose expression requires de novo protein synthesis Blood, March 1, 2003; 101(5): 1913 - 1918. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Liu, M. Catalfamo, Y. Li, P. A. Henkart, and N.-p. Weng IL-15 mimics T cell receptor crosslinking in the induction of cellular proliferation, gene expression, and cytotoxicity in CD8+ memory T cells PNAS, April 30, 2002; 99(9): 6192 - 6197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Liu, M. Catalfamo, Y. Li, P. A. Henkart, and N.-p. Weng IL-15 mimics T cell receptor crosslinking in the induction of cellular proliferation, gene expression, and cytotoxicity in CD8+ memory T cells PNAS, April 30, 2002; 99(9): 6192 - 6197. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
