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The Journal of Immunology, 2001, 166: 7009-7013.
Copyright © 2001 by The American Association of Immunologists

CUTTING EDGE

Cutting Edge: Lipid Raft Integrity Affects the Efficiency of MHC Class I Tetramer Binding and Cell Surface TCR Arrangement on CD8+ T Cells1

Donald R. Drake, III* and Thomas J. Braciale2,*,{dagger}

* Beirne B. Carter Center for Immunology Research, and {dagger} Departments of Pathology and Microbiology, University of Virginia, Charlottesville, VA 22908

Physically distinct cholesterol/sphingolipid-rich plasma membrane microdomains, so-called lipid rafts, have been recognized to play an important regulatory role in various cellular processes, from membrane trafficking to signal transduction, in a number of cell types. We report here that the ability of TCR on activated, functional CD8+ T lymphocytes to efficiently bind MHC class I tetramer complexes is dependent on the integrity of lipid rafts on the T lymphocyte membrane. We further provide evidence that TCR interact (associate) with lipid raft elements on the T cell surface before receptor engagement and that the topological arrangement of TCR on the cell surface is likewise influenced by lipid raft integrity.




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