| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Physiology Program, Harvard School of Public Health, Boston, MA 02115; and
Department of Pediatrics, Rainbow Babies and Children’s Hospital, Case Western Reserve University, Cleveland, OH 44106
Changes in the cytoskeleton of endothelial cells (ECs) play
important roles in mediating neutrophil migration during inflammation.
Previous studies demonstrated that neutrophil adherence to
TNF-
-treated pulmonary microvascular ECs induced cytoskeletal
remodeling in ECs that required ICAM-1 ligation and oxidant production
and was mimicked by cross-linking ICAM-1. In this study, we examined
the role of ICAM-1-induced signaling pathways in mediating actin
cytoskeletal remodeling. Cross-linking ICAM-1 induced alterations in
ICAM-1 distribution, as well as the filamentous actin rearrangements
and stiffening of ECs shown previously. ICAM-1 cross-linking induced
phosphorylation of the p38 mitogen-activated protein kinase (MAPK) that
was inhibited by allopurinol and also induced an increase in the
activity of the p38 MAPK that was inhibited by SB203580. However,
SB203580 had no effect on oxidant production in ECs or ICAM-1
clustering. ICAM-1 cross-linking also induced phosphorylation of heat
shock protein 27, an actin-binding protein that may be involved in
filamentous actin polymerization. The time course of heat shock protein
27 phosphorylation paralleled that of p38 MAPK phosphorylation and was
completely inhibited by SB203580. In addition, SB203580 blocked the EC
stiffening response induced by either neutrophil adherence or ICAM-1
cross-linking. Moreover, pretreatment of ECs with SB203580 reduced
neutrophil migration toward EC junctions. Taken together, these data
demonstrate that activation of p38 MAPK, mediated by xanthine
oxidase-generated oxidant production, is required for cytoskeletal
remodeling in ECs induced by ICAM-1 cross-linking or neutrophil
adherence. These cytoskeletal changes in ECs may in turn modulate
neutrophil migration toward EC junctions.
This article has been cited by other articles:
|
|
 |
|
  R. M. Rao, L. Yang, G. Garcia-Cardena, and F. W. Luscinskas Endothelial-Dependent Mechanisms of Leukocyte Recruitment to the Vascular Wall Circ. Res., August 3, 2007; 101(3): 234 - 247. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Urquhart, G. Rosignoli, D. Cooper, R. Motterlini, and M. Perretti Carbon Monoxide-Releasing Molecules Modulate Leukocyte-Endothelial Interactions under Flow J. Pharmacol. Exp. Ther., May 1, 2007; 321(2): 656 - 662. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. L. Korpi-Steiner, M. E. Bates, W.-M. Lee, D. J. Hall, and P. J. Bertics Human rhinovirus induces robust IP-10 release by monocytic cells, which is independent of viral replication but linked to type I interferon receptor ligation and STAT1 activation J. Leukoc. Biol., December 1, 2006; 80(6): 1364 - 1374. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Yang, J. R. Kowalski, P. Yacono, M. Bajmoczi, S. K. Shaw, R. M. Froio, D. E. Golan, S. M. Thomas, and F. W. Luscinskas Endothelial Cell Cortactin Coordinates Intercellular Adhesion Molecule-1 Clustering and Actin Cytoskeleton Remodeling during Polymorphonuclear Leukocyte Adhesion and Transmigration J. Immunol., November 1, 2006; 177(9): 6440 - 6449. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Koss, G. R. Pfeiffer II, Y. Wang, S. T. Thomas, M. Yerukhimovich, W. A. Gaarde, C. M. Doerschuk, and Q. Wang Ezrin/Radixin/Moesin Proteins Are Phosphorylated by TNF-{alpha} and Modulate Permeability Increases in Human Pulmonary Microvascular Endothelial Cells J. Immunol., January 15, 2006; 176(2): 1218 - 1227. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hu, S. P. RamachandraRao, S. Siva, C. Valancius, Y. Zhu, K. Mahadev, I. Toh, B. J. Goldstein, M. Woolkalis, and K. Sharma Reactive oxygen species production via NADPH oxidase mediates TGF-{beta}-induced cytoskeletal alterations in endothelial cells Am J Physiol Renal Physiol, October 1, 2005; 289(4): F816 - F825. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Fujiwara, E. Jin, M. Ghazizadeh, and O. Kawanami Activation of PAR4 Induces a Distinct Actin Fiber Formation via p38 MAPK in Human Lung Endothelial Cells J. Histochem. Cytochem., September 1, 2005; 53(9): 1121 - 1129. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Yang, R. M. Froio, T. E. Sciuto, A. M. Dvorak, R. Alon, and F. W. Luscinskas ICAM-1 regulates neutrophil adhesion and transcellular migration of TNF-{alpha}-activated vascular endothelium under flow Blood, July 15, 2005; 106(2): 584 - 592. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Cook-Mills and T. L. Deem Active participation of endothelial cells in inflammation J. Leukoc. Biol., April 1, 2005; 77(4): 487 - 495. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. N. Baranova, T. G. Vishnyakova, A. V. Bocharov, R. Kurlander, Z. Chen, M. L. Kimelman, A. T. Remaley, G. Csako, F. Thomas, T. L. Eggerman, et al. Serum Amyloid A Binding to CLA-1 (CD36 and LIMPII Analogous-1) Mediates Serum Amyloid A Protein-induced Activation of ERK1/2 and p38 Mitogen-activated Protein Kinases J. Biol. Chem., March 4, 2005; 280(9): 8031 - 8040. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. R. Curran, R. K. Morgan, P. J. Kingham, N. Durcan, W. G. McLean, M. T. Walsh, and R. W. Costello Mechanism of eosinophil induced signaling in cholinergic IMR-32 cells Am J Physiol Lung Cell Mol Physiol, February 1, 2005; 288(2): L326 - L332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Wang, M. Yerukhimovich, W. A. Gaarde, I. J. Popoff, and C. M. Doerschuk MKK3 and -6-dependent activation of p38{alpha} MAP kinase is required for cytoskeletal changes in pulmonary microvascular endothelial cells induced by ICAM-1 ligation Am J Physiol Lung Cell Mol Physiol, February 1, 2005; 288(2): L359 - L369. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Yu, D. Bian, C. Mahanivong, R. K. Cheng, W. Zhou, and S. Huang p38 Mitogen-activated Protein Kinase Regulation of Endothelial Cell Migration Depends on Urokinase Plasminogen Activator Expression J. Biol. Chem., November 26, 2004; 279(48): 50446 - 50454. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. van Buul and P. L. Hordijk Signaling in Leukocyte Transendothelial Migration Arterioscler. Thromb. Vasc. Biol., May 1, 2004; 24(5): 824 - 833. [Abstract] [Full Text]
|
|
|
|
|
|
C. G. Kevil, A. W. Orr, W. Langston, K. Mickett, J. Murphy-Ullrich, R. P. Patel, D. F. Kucik{ddagger}{ddagger}, and D. C. Bullard Intercellular Adhesion Molecule-1 (ICAM-1) Regulates Endothelial Cell Motility through a Nitric Oxide-dependent Pathway J. Biol. Chem., April 30, 2004; 279(18): 19230 - 19238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. V. Usatyuk and V. Natarajan Role of Mitogen-activated Protein Kinases in 4-Hydroxy-2-nonenal-induced Actin Remodeling and Barrier Function in Endothelial Cells J. Biol. Chem., March 19, 2004; 279(12): 11789 - 11797. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Wang, G. R. Pfeiffer II, and W. A. Gaarde Activation of SRC Tyrosine Kinases in Response to ICAM-1 Ligation in Pulmonary Microvascular Endothelial Cells J. Biol. Chem., November 28, 2003; 278(48): 47731 - 47743. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Lyck, Y. Reiss, N. Gerwin, J. Greenwood, P. Adamson, and B. Engelhardt T-cell interaction with ICAM-1/ICAM-2 double-deficient brain endothelium in vitro: the cytoplasmic tail of endothelial ICAM-1 is necessary for transendothelial migration of T cells Blood, November 15, 2003; 102(10): 3675 - 3683. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Kingham, W. G. McLean, M.-T. Walsh, A. D. Fryer, G. J. Gleich, and R. W. Costello Effects of eosinophils on nerve cell morphology and development: the role of reactive oxygen species and p38 MAP kinase Am J Physiol Lung Cell Mol Physiol, October 1, 2003; 285(4): L915 - L924. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. R. Alevriadou CAMs and Rho small GTPases: gatekeepers for leukocyte transendothelial migration. Focus on "VCAM-1-mediated Rac signaling controls endothelial cell-cell contacts and leukocyte transmigration" Am J Physiol Cell Physiol, August 1, 2003; 285(2): C250 - C252. [Full Text] [PDF]
|
|
|
|
 |
|
 A. R. Burns, C. W. Smith, and D. C. Walker Unique Structural Features That Influence Neutrophil Emigration Into the Lung Physiol Rev, April 1, 2003; 83(2): 309 - 336. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Blaber, E. Stylianou, A. Clayton, and R. Steadman Selective Regulation of ICAM-1 and RANTES Gene Expression after ICAM-1 Ligation on Human Renal Fibroblasts J. Am. Soc. Nephrol., January 1, 2003; 14(1): 116 - 127. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. Mizgerd, M. R. Spieker, and M. M. Lupa Exon truncation by alternative splicing of murine ICAM-1 Physiol Genomics, December 26, 2002; 12(1): 47 - 51. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Vliagoftis Thrombin Induces Mast Cell Adhesion to Fibronectin: Evidence for Involvement of Protease-Activated Receptor-1 J. Immunol., October 15, 2002; 169(8): 4551 - 4558. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Wang, G. R. Pfeiffer II, T. Stevens, and C. M. Doerschuk Lung Microvascular and Arterial Endothelial Cells Differ in Their Responses to Intercellular Adhesion Molecule-1 Ligation Am. J. Respir. Crit. Care Med., September 15, 2002; 166(6): 872 - 877. [Abstract] [Full Text]
|
|
|
|
|
|
J. D. van Buul, C. Voermans, V. van den Berg, E. C. Anthony, F. P. J. Mul, S. van Wetering, C. E. van der Schoot, and P. L. Hordijk Migration of Human Hematopoietic Progenitor Cells Across Bone Marrow Endothelium Is Regulated by Vascular Endothelial Cadherin J. Immunol., January 15, 2002; 168(2): 588 - 596. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. Cara, J. Kaur, M. Forster, D.-M. McCafferty, and P. Kubes Role of p38 Mitogen-Activated Protein Kinase in Chemokine-Induced Emigration and Chemotaxis In Vivo J. Immunol., December 1, 2001; 167(11): 6552 - 6558. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Kingham, W. G. McLean, D. A. Sawatzky, M. T. Walsh, and R. W. Costello Adhesion-dependent interactions between eosinophils and cholinergic nerves Am J Physiol Lung Cell Mol Physiol, June 1, 2002; 282(6): L1229 - L1238. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
