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-Chain Gene Rearrangement Correlates with Its Association with the Underphosphorylated Form of Retinoblastoma1
Department of Life Science, Tokyo Institute of Technology, Yokohama, Japan
The KI and KII sites play a crucial role in 
-chain gene
rearrangement, which was investigated in mice deficient for these
sites. Previously, we found that Pax-5 can bind to the KI and KII
sites; however, the function of Pax-5 in -chain gene rearrangement
has not been investigated. Here, we have used an in vitro culture
system in which differentiation from pre-B cells to immature B cells is
induced by removing IL-7. We showed that, after the induction of
differentiation, Pax-5 dissociated from the KI and KII revealed by EMSA
analyses, and this dissociation occurred specifically at the KI and KII
sites, but not at the Pax-5 binding site, in the CD19 promoter because
of a lower binding affinity of Pax-5 for the KI and KII sites. During
differentiation induced by removing IL-7, the underphosphorylated form
of retinoblastoma preferentially associated with Pax-5, which caused
dissociation of Pax-5 from KI and KII sites. These results suggest that
the dissociation of Pax-5 from the KI and KII sites is important in the
induction of -chain gene rearrangement.
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