| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Biology, Johns Hopkins University, Baltimore, MD 21218
Fluorescence resonance energy transfer between cyan fluorescent protein- and yellow fluorescent protein-tagged MHC class I molecules reports on their spatial organization during assembly and export from the endoplasmic reticulum (ER). A fraction of MHC class I molecules is clustered in the ER at steady state. Contrary to expectations from biochemical models, this fraction is not bound to the TAP. Instead, it appears that MHC class I molecules cluster after peptide loading. This clustering points toward a novel step involved in the selective export of peptide-loaded MHC class I molecules from the ER. Consistent with this model, we detected clusters of wild-type HLA-A2 molecules and of mutant A2-T134K molecules that cannot bind TAP, but HLA-A2 did not detectably cluster with A2-T134K at steady state. Lactacystin treatment disrupted the HLA-A2 clusters, but had no effect on the A2-T134K clusters. However, when cells were fed peptides with high affinity for HLA-A2, mixed clusters containing both HLA-A2 and A2-T134K were detected.
This article has been cited by other articles:
|
|
 |
|
  L. Martinez Munoz, P. Lucas, G. Navarro, A. I. Checa, R. Franco, C. Martinez-A., J. M. Rodriguez-Frade, and M. Mellado Dynamic Regulation of CXCR1 and CXCR2 Homo- and Heterodimers J. Immunol., December 1, 2009; 183(11): 7337 - 7346. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Bocksteins, A. J. Labro, E. Mayeur, T. Bruyns, J.-P. Timmermans, D. Adriaensen, and D. J. Snyders Conserved Negative Charges in the N-terminal Tetramerization Domain Mediate Efficient Assembly of Kv2.1 and Kv2.1/Kv6.4 Channels J. Biol. Chem., November 13, 2009; 284(46): 31625 - 31634. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Abe, N. Van Prooyen, J. J. Ladasky, and M. Edidin Interaction of Bap31 and MHC Class I Molecules and Their Traffic Out of the Endoplasmic Reticulum J. Immunol., April 15, 2009; 182(8): 4776 - 4783. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Bremond, O. Meynet, K. Mahiddine, S. Coito, M. Tichet, K. Scotlandi, J.-P. Breittmayer, P. Gounon, P. A. Gleeson, A. Bernard, et al. Regulation of HLA class I surface expression requires CD99 and p230/golgin-245 interaction Blood, January 8, 2009; 113(2): 347 - 357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Tuli, M. Sharma, M. M. McIlhaney, J. E. Talmadge, N. Naslavsky, S. Caplan, and J. C. Solheim Amyloid Precursor-Like Protein 2 Increases the Endocytosis, Instability, and Turnover of the H2-Kd MHC Class I Molecule J. Immunol., August 1, 2008; 181(3): 1978 - 1987. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Chichili and W. Rodgers Clustering of Membrane Raft Proteins by the Actin Cytoskeleton J. Biol. Chem., December 14, 2007; 282(50): 36682 - 36691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. W. Everett and M. Edidin Tapasin Increases Efficiency of MHC I Assembly in the Endoplasmic Reticulum but Does Not Affect MHC I Stability at the Cell Surface J. Immunol., December 1, 2007; 179(11): 7646 - 7652. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Wallrabe, G. Bonamy, A. Periasamy, and M. Barroso Receptor Complexes Cotransported via Polarized Endocytic Pathways Form Clusters with Distinct Organizations Mol. Biol. Cell, June 1, 2007; 18(6): 2226 - 2243. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Thomas, C. A. Nechamen, J. E. Mazurkiewicz, M. Muda, S. Palmer, and J. A. Dias Follice-Stimulating Hormone Receptor Forms Oligomers and Shows Evidence of Carboxyl-Terminal Proteolytic Processing Endocrinology, May 1, 2007; 148(5): 1987 - 1995. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Ladasky, S. Boyle, M. Seth, H. Li, T. Pentcheva, F. Abe, S. J. Steinberg, and M. Edidin Bap31 Enhances the Endoplasmic Reticulum Export and Quality Control of Human Class I MHC Molecules J. Immunol., November 1, 2006; 177(9): 6172 - 6181. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Chattopadhyay, S. Bhatia, A. Fiser, S. C. Almo, and S. G. Nathenson Structural Basis of Inducible Costimulator Ligand Costimulatory Function: Determination of the Cell Surface Oligomeric State and Functional Mapping of the Receptor Binding Site of the Protein J. Immunol., September 15, 2006; 177(6): 3920 - 3929. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Philip and S. Scarlata Real-Time Measurements of Protein Affinities on Membrane Surfaces by Fluorescence Spectroscopy Sci. Signal., August 29, 2006; 2006(350): pl5 - pl5. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. H. Boyle, A. K. Gillingham, S. Munro, and J. Trowsdale Selective Export of HLA-F by Its Cytoplasmic Tail. J. Immunol., June 1, 2006; 176(11): 6464 - 6472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R. Fooksman, G. K. Gronvall, Q. Tang, and M. Edidin Clustering Class I MHC Modulates Sensitivity of T Cell Recognition. J. Immunol., June 1, 2006; 176(11): 6673 - 6680. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Nobles, A. Benians, and A. Tinker Heterotrimeric G proteins precouple with G protein-coupled receptors in living cells PNAS, December 20, 2005; 102(51): 18706 - 18711. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bhatia, M. Edidin, S. C. Almo, and S. G. Nathenson Different cell surface oligomeric states of B7-1 and B7-2: Implications for signaling PNAS, October 25, 2005; 102(43): 15569 - 15574. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Benians, M. Nobles, S. Hosny, and A. Tinker Regulators of G-protein Signaling Form a Quaternary Complex with the Agonist, Receptor, and G-protein: A NOVEL EXPLANATION FOR THE ACCELERATION OF SIGNALING ACTIVATION KINETICS J. Biol. Chem., April 8, 2005; 280(14): 13383 - 13394. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-E. Paquet, M. Cohen-Doyle, G. C. Shore, and D. B. Williams Bap29/31 Influences the Intracellular Traffic of MHC Class I Molecules J. Immunol., June 15, 2004; 172(12): 7548 - 7555. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. M. PAULSSON and P. WANG Quality control of MHC class I maturation FASEB J, January 1, 2004; 18(1): 31 - 38. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Faure, D. F. Barber, S. M. Takahashi, T. Jin, and E. O. Long Spontaneous Clustering and Tyrosine Phosphorylation of NK Cell Inhibitory Receptor Induced by Ligand Binding J. Immunol., June 15, 2003; 170(12): 6107 - 6114. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Williams, J. F. Roeth, M. R. Kasper, R. I. Fleis, C. G. Przybycin, and K. L. Collins Direct Binding of Human Immunodeficiency Virus Type 1 Nef to the Major Histocompatibility Complex Class I (MHC-I) Cytoplasmic Tail Disrupts MHC-I Trafficking J. Virol., October 25, 2002; 76(23): 12173 - 12184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Issafras, S. Angers, S. Bulenger, C. Blanpain, M. Parmentier, C. Labbe-Jullie, M. Bouvier, and S. Marullo Constitutive Agonist-independent CCR5 Oligomerization and Antibody-mediated Clustering Occurring at Physiological Levels of Receptors J. Biol. Chem., September 13, 2002; 277(38): 34666 - 34673. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. T. Spiliotis, T. Pentcheva, and M. Edidin Probing for Membrane Domains in the Endoplasmic Reticulum: Retention and Degradation of Unassembled MHC Class I Molecules Mol. Biol. Cell, May 1, 2002; 13(5): 1566 - 1581. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Pentcheva, E. T. Spiliotis, and M. Edidin Cutting Edge: Tapasin Is Retained in the Endoplasmic Reticulum by Dynamic Clustering and Exclusion from Endoplasmic Reticulum Exit Sites J. Immunol., February 15, 2002; 168(4): 1538 - 1541. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
