HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Petersson, K. Articles by Ivars, F. Search for Related Content PubMed PubMed Citation Articles by Petersson, K. Articles by Ivars, F.

Early TCR Expression Promotes Maturation of T Cells Expressing FcRI Containing TCR/CD3 Complexes¹

Section for Immunology, Department of Cell and Molecular Biology, Lund University, Lund, Sweden

In a previous study we presented data indicating that the expanded population of CD4^-CD8^- (DN) T cells in TCR-chain-transgenic mice was partially if not entirely derived from T cell lineage cells. The development of both T cells and DN T cells is poorly understood; therefore, we thought it would be important to identify the immediate precursors of the transgene-induced DN T cells. We have in this report studied the early T cell development in these mice and we show that the transgenic TCR-chain is expressed by precursor thymocytes already at the CD3^-CD4^-CD8^- (triple negative, TN) CD44⁺CD25^- stage of development. Both by using purified precursor populations in reconstitution experiments and by analyzing fetal thymocyte development, we demonstrated that early TN precursors expressing endogenous TCR-chains matured into DN T cells at several stages of development. The genes encoding the -chain of the high affinity receptor for IgE (FcRI) and the CD3 protein were found to be reciprocally expressed in TN thymocytes such that during development the FcRI expression decreased whereas CD3 expression increased. Furthermore, in a fraction of the transgene-induced DN T cells the FcRI protein colocalized with the TCR/CD3 complex. These data suggest that similarly to T cells and NKT cells, precursors expressing the TCR early in the common developmental pathway may use the FcRI protein as a signaling component of the TCR/CD3 complex.

This article has been cited by other articles:

				K. N. Taylor, V. R. Shinde Patil, and Y. L. Colson Reconstitution of Allogeneic Hemopoietic Stem Cells: The Essential Role of FcR{gamma} and the TCR beta-Chain-FCp33 Complex J. Immunol., August 1, 2006; 177(3): 1444 - 1450. [Abstract] [Full Text] [PDF]

				B. P.-L. Lee, E. Mansfield, S.-C. Hsieh, T. Hernandez-Boussard, W. Chen, C. W. Thomson, M. S. Ford, S. E. Bosinger, S. Der, Z.-x. Zhang, et al. Expression Profiling of Murine Double-Negative Regulatory T Cells Suggest Mechanisms for Prolonged Cardiac Allograft Survival J. Immunol., April 15, 2005; 174(8): 4535 - 4544. [Abstract] [Full Text] [PDF]

				P. Gadue, L. Yin, S. Jain, and P. L. Stein Restoration of NK T Cell Development in fyn-Mutant Mice by a TCR Reveals a Requirement for Fyn During Early NK T Cell Ontogeny J. Immunol., May 15, 2004; 172(10): 6093 - 6100. [Abstract] [Full Text] [PDF]

				J. M. Jameson, G. Cauvi, D. A. Witherden, and W. L. Havran A Keratinocyte-Responsive {gamma}{delta} TCR Is Necessary for Dendritic Epidermal T Cell Activation by Damaged Keratinocytes and Maintenance in the Epidermis J. Immunol., March 15, 2004; 172(6): 3573 - 3579. [Abstract] [Full Text] [PDF]