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The Journal of Immunology, 2001, 166: 6323-6331.
Copyright © 2001 by The American Association of Immunologists

IL-10-Deficient Mice Demonstrate Multiple Organ Failure and Increased Mortality During Escherichia coli Peritonitis Despite an Accelerated Bacterial Clearance1

Miguel E. Sewnath*, Dariusz P. Olszyna{dagger},{ddagger}, Rakesh Birjmohun*, Fiebo J. W. ten Kate§, Dirk J. Gouma* and Tom van der Poll{dagger},{ddagger}

Departments of * Surgery, {dagger} Experimental Internal Medicine, {ddagger} Infectious Diseases, Tropical Medicine and AIDS, and § Pathology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands

To determine the role of endogenous IL-10 in local antibacterial host defense and in the development of a systemic inflammatory response syndrome during abdominal sepsis, IL-10 gene-deficient (IL-10-/-) and wild-type (IL-10+/+) mice received an i.p. injection with Escherichia coli. Peritonitis was associated with a bacterial dose-dependent increase in IL-10 concentrations in peritoneal fluid and plasma. The recovery of E. coli from the peritoneal fluid, blood, and lungs was diminished in IL-10-/- mice, indicating that endogenous IL-10 impaired bacterial clearance. Despite a lower bacterial load, IL-10-/- mice had higher concentrations of TNF, macrophage inflammatory protein-2 and keratinocyte in peritoneal fluid and plasma, and demonstrated more severe multiple organ damage as indicated by clinical chemistry and histopathology. Furthermore, IL-10-/- mice showed an increased neutrophil recruitment to the peritoneal cavity. To examine the role of elevated TNF levels in the altered host response in IL-10-/- mice, the effect of a neutralizing anti-TNF mAb was determined. Anti-TNF did not influence the clearance of E. coli in either IL-10+/+ or IL-10-/- mice. Furthermore, anti-TNF did not affect leukocyte influx in the peritoneal fluid, multiple organ damage, or survival in IL-10+/+ mice. In IL-10-/- mice, anti-TNF partially attenuated neutrophil recruitment and multiple organ damage, and prevented the increased lethality. These data suggest that although endogenous IL-10 facilitates the outgrowth and dissemination of bacteria during E. coli peritonitis, it protects mice from lethality by attenuating the development of a systemic inflammatory response syndrome by a mechanism that involves inhibition of TNF release.




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