| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
,
Departments of
*
Anesthesia,
Medicine, and
Surgery, and
Cardiovascular Research Institute, University of California San Francisco, CA 94110; and
¶
Department of Comparative Biosciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706
Our recent experimental work demonstrated that a
neutrophil-dependent inflammatory response in the lung prevented the
normal up-regulation of alveolar fluid clearance by catecholamines
following hemorrhagic shock. In this study, we tested the hypothesis
that the release of NO within the airspaces of the lung was responsible
for the shock-mediated failure of the alveolar epithelium to respond to
catecholamines in rats. Hemorrhagic shock was associated with an
inducible NO synthase (iNOS)-dependent increase in the lung production
of NO and a failure of the alveolar epithelium to up-regulate vectorial
fluid transport in response to 
-adrenergic agonists. Inhibition of
iNOS restored the normal catecholamine-mediated up-regulation of
alveolar liquid clearance. Airspace instillation of dibutyryl cAMP, a
stable analog of cAMP, restored the normal fluid transport capacity of
the alveolar epithelium after prolonged hemorrhagic shock, whereas
direct stimulation of adenyl cyclase by forskolin had no effect.
Pretreatment with pyrrolidine dithiocarbamate or sulfasalazine
attenuated the iNOS-dependent production of NO in the lung and restored
the normal up-regulation of alveolar fluid clearance by catecholamines
after prolonged hemorrhagic shock. Based on in vitro studies with an
alveolar epithelial cell line, A549 cells, the effect of sulfasalazine
appeared to be mediated in part by inhibition of NF-
B activation,
and the protective effect was mediated by the inhibition of IB
protein degradation. In summary, these results provide the first in
vivo evidence that NO, released within the airspaces of the lung
probably secondary to the NF-B-dependent activation of iNOS, is a
major proximal inflammatory mediator that limits the rate of alveolar
epithelial transport after prolonged hemorrhagic shock by directly
impairing the function of membrane proteins involved in the
-adrenergic receptor-cAMP signaling pathway in alveolar
epithelium.
This article has been cited by other articles:
|
|
 |
|
  G. M. Mutlu and P. Factor Alveolar Epithelial 2-Adrenergic Receptors Am. J. Respir. Cell Mol. Biol., February 1, 2008; 38(2): 127 - 134. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Song and S. Matalon Modulation of alveolar fluid clearance by reactive oxygen-nitrogen intermediates Am J Physiol Lung Cell Mol Physiol, October 1, 2007; 293(4): L855 - L858. [Full Text] [PDF]
|
|
|
|
 |
|
 J. W. Lee, X. Fang, G. Dolganov, R. D. Fremont, J. A. Bastarache, L. B. Ware, and M. A. Matthay Acute Lung Injury Edema Fluid Decreases Net Fluid Transport across Human Alveolar Epithelial Type II Cells J. Biol. Chem., August 17, 2007; 282(33): 24109 - 24119. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Matthay and H. G. Folkesson Reduction of Alveolar Epithelial Ion and Fluid Transport by Inflammatory Mediators Am. J. Respir. Cell Mol. Biol., March 1, 2007; 36(3): 389 - 389. [Full Text] [PDF]
|
|
|
|
 |
|
 B. Geny, H. Khun, C. Fitting, L. Zarantonelli, C. Mazuet, N. Cayet, M. Szatanik, M.-C. Prevost, J.-M. Cavaillon, M. Huerre, et al. Clostridium sordellii Lethal Toxin Kills Mice by Inducing a Major Increase in Lung Vascular Permeability Am. J. Pathol., March 1, 2007; 170(3): 1003 - 1017. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G D Perkins, N Nathani, D F McAuley, F Gao, and D R Thickett In vitro and in vivo effects of salbutamol on neutrophil function in acute lung injury Thorax, January 1, 2007; 62(1): 36 - 42. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Godzich, M. Hodnett, J. A. Frank, G. Su, M. Pespeni, A. Angel, M. B. Howard, M. A. Matthay, and J. F. Pittet Activation of the stress protein response prevents the development of pulmonary edema by inhibiting VEGF cell signaling in a model of lung ischemia-reperfusion injury in rats FASEB J, July 1, 2006; 20(9): 1519 - 1521. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Li and H. G. Folkesson RNA interference for {alpha}-ENaC inhibits rat lung fluid absorption in vivo Am J Physiol Lung Cell Mol Physiol, April 1, 2006; 290(4): L649 - L660. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. D. Perkins, D. F. McAuley, D. R. Thickett, and F. Gao The beta-Agonist Lung Injury Trial (BALTI): A Randomized Placebo-controlled Clinical Trial Am. J. Respir. Crit. Care Med., February 1, 2006; 173(3): 281 - 287. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Minamoto, H. Harada, V. N. Lama, M. A. Fedarau, and D. J. Pinsky Reciprocal regulation of airway rejection by the inducible gas-forming enzymes heme oxygenase and nitric oxide synthase J. Exp. Med., July 18, 2005; 202(2): 283 - 294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Roux, H. Kawakatsu, B. Gartland, M. Pespeni, D. Sheppard, M. A. Matthay, C. M. Canessa, and J.-F. Pittet Interleukin-1{beta} Decreases Expression of the Epithelial Sodium Channel {alpha}-Subunit in Alveolar Epithelial Cells via a p38 MAPK-dependent Signaling Pathway J. Biol. Chem., May 13, 2005; 280(19): 18579 - 18589. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. E. Parsons, M. A. Matthay, L. B. Ware, M. D. Eisner, and and the National Heart, Lung, Blood Institute Acut Elevated plasma levels of soluble TNF receptors are associated with morbidity and mortality in patients with acute lung injury Am J Physiol Lung Cell Mol Physiol, March 1, 2005; 288(3): L426 - L431. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Mutlu, W. J. Koch, and P. Factor Alveolar Epithelial {beta}2-Adrenergic Receptors: Their Role in Regulation of Alveolar Active Sodium Transport Am. J. Respir. Crit. Care Med., December 15, 2004; 170(12): 1270 - 1275. [Full Text] [PDF]
|
|
|
|
|
|
A. Ishizaka, T. Matsuda, K. H. Albertine, H. Koh, S. Tasaka, N. Hasegawa, N. Kohno, T. Kotani, H. Morisaki, J. Takeda, et al. Elevation of KL-6, a lung epithelial cell marker, in plasma and epithelial lining fluid in acute respiratory distress syndrome Am J Physiol Lung Cell Mol Physiol, June 1, 2004; 286(6): L1088 - L1094. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Han, M. P. Fink, T. Uchiyama, R. Yang, and R. L. Delude Increased iNOS activity is essential for pulmonary epithelial tight junction dysfunction in endotoxemic mice Am J Physiol Lung Cell Mol Physiol, February 1, 2004; 286(2): L259 - L267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Frank, J. Roux, H. Kawakatsu, G. Su, A. Dagenais, Y. Berthiaume, M. Howard, C. M. Canessa, X. Fang, D. Sheppard, et al. Transforming Growth Factor-{beta}1 Decreases Expression of the Epithelial Sodium Channel {alpha}ENaC and Alveolar Epithelial Vectorial Sodium and Fluid Transport via an ERK1/2-dependent Mechanism J. Biol. Chem., November 7, 2003; 278(45): 43939 - 43950. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. B. Ware, X. Fang, and M. A. Matthay Protein C and thrombomodulin in human acute lung injury Am J Physiol Lung Cell Mol Physiol, September 1, 2003; 285(3): L514 - L521. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Frank, J.-F. Pittet, H. Lee, M. Godzich, and M. A. Matthay High tidal volume ventilation induces NOS2 and impairs cAMP- dependent air space fluid clearance Am J Physiol Lung Cell Mol Physiol, May 1, 2003; 284(5): L791 - L798. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Matthay Alveolar Fluid Clearance in Patients With ARDS: Does It Make a Difference? Chest, December 1, 2002; 122(6_suppl): 340S - 343S. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Sartori and M.A. Matthay Alveolar epithelial fluid transport in acute lung injury: new insights Eur. Respir. J., November 1, 2002; 20(5): 1299 - 1313. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. B. Ware, X. Fang, Y. Wang, T. Sakuma, T. S. Hall, and M. A. Matthay Lung Edema Clearance: 20 Years of Progress: Selected Contribution: Mechanisms that may stimulate the resolution of alveolar edema in the transplanted human lung J Appl Physiol, November 1, 2002; 93(5): 1869 - 1874. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Pham, T. Uchida, C. Planes, L. B. Ware, R. Kaner, M. A. Matthay, and C. Clerici Hypoxia upregulates VEGF expression in alveolar epithelial cells in vitro and in vivo Am J Physiol Lung Cell Mol Physiol, November 1, 2002; 283(5): L1133 - L1142. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Matthay, H. G. Folkesson, and C. Clerici Lung Epithelial Fluid Transport and the Resolution of Pulmonary Edema Physiol Rev, July 1, 2002; 82(3): 569 - 600. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. R. Pitt and C. M. St. Croix Complex Regulation of iNOS in Lung Am. J. Respir. Cell Mol. Biol., January 1, 2002; 26(1): 6 - 9. [Full Text] [PDF]
|
|
|
|
|
|
M. L. Vivona, M. Matthay, M. B. Chabaud, G. Friedlander, and C. Clerici Hypoxia Reduces Alveolar Epithelial Sodium and Fluid Transport in Rats . Reversal by beta -Adrenergic Agonist Treatment Am. J. Respir. Cell Mol. Biol., November 1, 2001; 25(5): 554 - 561. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Dumasius, J. I. Sznajder, Z. S. Azzam, J. Boja, G. M. Mutlu, M. B. Maron, and P. Factor {beta}2-Adrenergic Receptor Overexpression Increases Alveolar Fluid Clearance and Responsiveness to Endogenous Catecholamines in Rats Circ. Res., November 9, 2001; 89(10): 907 - 914. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
