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*
Immunology Division and
Transplantation and Autoimmunity Division, The Walter and Eliza Hall Institute of Medical Research, P.O. Royal Melbourne Hospital, Parkville, Victoria, Australia;
The Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia;
The Department of Pathology and Immunology, Monash Medical School, Prahran, Victoria, Australia; and
¶ Department of Nephrology and Immunology, Clinic of the Rheinisch-Westfälische Technische Hochschule, Aachen, Germany
To better understand the antigenic requirements for cross-presentation, we compared the in vivo efficiency of presentation of cell-associated vs soluble OVA with the OT-I (CD8) and OT-II (CD4) TCR transgenic lines. Cross-presentation of cell-associated OVA was very efficient, requiring as little as 21 ng of OVA to activate OT-II cells and 100-fold less to activate OT-I cells. In contrast, soluble OVA was presented inefficiently, requiring at least 10,000 ng OVA for activation of either T cell subset. Thus, cell-associated OVA was presented 500-fold more efficiently than soluble OVA to CD4 T cells and 50,000-fold more efficiently to CD8 T cells. These data, which represent the first quantitative in vivo analysis of cross-presentation, show that cell-associated OVA is very efficiently presented via the class I pathway.
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J. de Jersey, D. Carmignac, T. Barthlott, I. Robinson, and B. Stockinger Activation of CD8 T Cells by Antigen Expressed in the Pituitary Gland J. Immunol., December 15, 2002; 169(12): 6753 - 6759. [Abstract] [Full Text] [PDF]
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C. Rush, T. Mitchell, and P. Garside Efficient Priming of CD4+ and CD8+ T Cells by DNA Vaccination Depends on Appropriate Targeting of Sufficient Levels of Immunologically Relevant Antigen to Appropriate Processing Pathways J. Immunol., November 1, 2002; 169(9): 4951 - 4960. [Abstract] [Full Text] [PDF]
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K. Liu, T. Iyoda, M. Saternus, Y. Kimura, K. Inaba, and R. M. Steinman Immune Tolerance After Delivery of Dying Cells to Dendritic Cells In Situ J. Exp. Med., October 21, 2002; 196(8): 1091 - 1097. [Abstract] [Full Text] [PDF]
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G. T. Belz, G. M.N. Behrens, C. M. Smith, J. F.A.P. Miller, C. Jones, K. Lejon, C. G. Fathman, S. N. Mueller, K. Shortman, F. R. Carbone, et al. The CD8{alpha}+ Dendritic Cell Is Responsible for Inducing Peripheral Self-Tolerance to Tissue-associated Antigens J. Exp. Med., October 21, 2002; 196(8): 1099 - 1104. [Abstract] [Full Text] [PDF]
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M. Skoberne, S. Schenk, H. Hof, and G. Geginat Cross-Presentation of Listeria monocytogenes-Derived CD4 T Cell Epitopes J. Immunol., August 1, 2002; 169(3): 1410 - 1418. [Abstract] [Full Text] [PDF]
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G. Moron, P. Rueda, I. Casal, and C. Leclerc CD8{alpha}2 CD11b+ Dendritic Cells Present Exogenous Virus-like Particles to CD8+ T Cells and Subsequently Express CD8{alpha} and CD205 Molecules J. Exp. Med., May 20, 2002; 195(10): 1233 - 1245. [Abstract] [Full Text] [PDF]
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 E. B. Olasz, J. Linton, and S. I. Katz Soluble proteins and haptens on bone marrow-derived dendritic cells are presented to host CD4 T cells in an MHC-restricted manner Int. Immunol., May 1, 2002; 14(5): 493 - 502. [Abstract] [Full Text] [PDF]
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J. H. Cho, J. W. Youn, and Y. C. Sung Cross-Priming as a Predominant Mechanism for Inducing CD8+ T Cell Responses in Gene Gun DNA Immunization J. Immunol., November 15, 2001; 167(10): 5549 - 5557. [Abstract] [Full Text] [PDF]
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