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Usage in Immature Thymocytes Is Independent of DJ Proximity and pT
Pairing1
Ludwig Institute for Cancer Research, Lausanne Branch, University of Lausanne, Epalinges, Switzerland
During thymus development, the TCR 
locus rearranges before the
TCR 
locus. Pairing of productively rearranged TCR -chains with
an invariant pT chain leads to the formation of a pre-TCR and
subsequent expansion of immature pre-T cells. Essentially nothing is
known about the TCR V repertoire in pre-T cells before or after the
expression of a pre-TCR. Using intracellular staining, we show here
that the TCR V repertoire is significantly biased at the earliest
developmental stage in which VDJ rearrangement has occurred.
Moreover (and in contrast to the VH repertoire in immature
B cells), V repertoire biases in immature T cells do not reflect
proximity of V gene segments to the DJ cluster, nor do they
depend upon preferential V pairing with the pT chain. We conclude
that V gene repertoires in developing T and B cells are controlled by
partially distinct mechanisms.
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