| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Department of Infectious Diseases and Microbiology and
Department of Immunology, Imperial College School of Medicine, St. Mary’s Campus, London, United Kingdom;
Glaxo-Wellcome Immunology Division, Stevenage, United Kingdom; and
Unité d’Oncologie Virale, Institut Pasteur, Paris, France
Following uptake by macrophages, live mycobacteria initially reside within an immature phagosome that resists acidification and retains access to recycling endosomes. Glycolipids are exported from the mycobacterial phagosome and become available for immune recognition by CD1-restricted T cells. The aim of this study was to explore the possibility that lipoproteins might similarly escape from the phagosome and act as immune targets in cells infected with live mycobacteria. We have focused on a 19-kDa lipoprotein from Mycobacterium tuberculosis that was previously shown to be recognized by CD8+ T cells. The 19-kDa Ag was found to traffic separately from live mycobacteria within infected macrophages by a pathway that was dependent on acylation of the protein. When expressed as a recombinant protein in rapid-growing mycobacteria, the 19-kDa Ag was able to deliver peptides for recognition by MHC class I-restricted T cells by a TAP-independent mechanism. Entry into the class I pathway was rapid, dependent on acylation, and could be blocked by killing the mycobacteria by heating before infection. Although the pattern of 19-kDa trafficking was similar with different mycobacterial species, preliminary experiments suggest that class I presentation is more efficient during infection with rapid-growing mycobacteria than with the slow-growing bacillus Calmette-Guérin vaccine strain.
This article has been cited by other articles:
|
|
 |
|
  M. Bastian, T. Braun, H. Bruns, M. Rollinghoff, and S. Stenger Mycobacterial Lipopeptides Elicit CD4+ CTLs in Mycobacterium tuberculosis-Infected Humans J. Immunol., March 1, 2008; 180(5): 3436 - 3446. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Barrionuevo, J. Cassataro, M. V. Delpino, A. Zwerdling, K. A. Pasquevich, C. G. Samartino, J. C. Wallach, C. A. Fossati, and G. H. Giambartolomei Brucella abortus Inhibits Major Histocompatibility Complex Class II Expression and Antigen Processing through Interleukin-6 Secretion via Toll-Like Receptor 2 Infect. Immun., January 1, 2008; 76(1): 250 - 262. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gandotra, S. Jang, P. J. Murray, P. Salgame, and S. Ehrt Nucleotide-Binding Oligomerization Domain Protein 2-Deficient Mice Control Infection with Mycobacterium tuberculosis Infect. Immun., November 1, 2007; 75(11): 5127 - 5134. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Narayana, B. Joshi, V. M. Katoch, K. C. Mishra, and K. N. Balaji Differential B-Cell Responses Are Induced by Mycobacterium tuberculosis PE Antigens Rv1169c, Rv0978c, and Rv1818c Clin. Vaccine Immunol., October 1, 2007; 14(10): 1334 - 1341. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E.-J. Im, N. Saubi, G. Virgili, C. Sander, D. Teoh, J. M. Gatell, H. McShane, J. Joseph, and T. Hanke Vaccine Platform for Prevention of Tuberculosis and Mother-to-Child Transmission of Human Immunodeficiency Virus Type 1 through Breastfeeding J. Virol., September 1, 2007; 81(17): 9408 - 9418. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.-H. Hovav, M. J. Cayabyab, M. W. Panas, S. Santra, J. Greenland, R. Geiben, B. F. Haynes, W. R. Jacobs Jr., and N. L. Letvin Rapid Memory CD8+ T-Lymphocyte Induction through Priming with Recombinant Mycobacterium smegmatis J. Virol., January 1, 2007; 81(1): 74 - 83. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Lewinsohn, J. E. Grotzke, A. S. Heinzel, L. Zhu, P. J. Ovendale, M. Johnson, and M. R. Alderson Secreted Proteins from Mycobacterium tuberculosis Gain Access to the Cytosolic MHC Class-I Antigen-Processing Pathway J. Immunol., July 1, 2006; 177(1): 437 - 442. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Torres, L. Ramachandra, R. E. Rojas, K. Bobadilla, J. Thomas, D. H. Canaday, C. V. Harding, and W. H. Boom Role of Phagosomes and Major Histocompatibility Complex Class II (MHC-II) Compartment in MHC-II Antigen Processing of Mycobacterium tuberculosis in Human Macrophages Infect. Immun., March 1, 2006; 74(3): 1621 - 1630. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Cayabyab, A.-H. Hovav, T. Hsu, G. R. Krivulka, M. A. Lifton, D. A. Gorgone, G. J. Fennelly, B. F. Haynes, W. R. Jacobs Jr., and N. L. Letvin Generation of CD8+ T-Cell Responses by a Recombinant Nonpathogenic Mycobacterium smegmatis Vaccine Vector Expressing Human Immunodeficiency Virus Type 1 Env J. Virol., February 15, 2006; 80(4): 1645 - 1652. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Sendide, A.-E. Deghmane, D. Pechkovsky, Y. Av-Gay, A. Talal, and Z. Hmama Mycobacterium bovis BCG Attenuates Surface Expression of Mature Class II Molecules through IL-10-Dependent Inhibition of Cathepsin S J. Immunol., October 15, 2005; 175(8): 5324 - 5332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Stewart, K. A. Wilkinson, S. M. Newton, S. M. Sullivan, O. Neyrolles, J. R. Wain, J. Patel, K.-L. Pool, D. B. Young, and R. J. Wilkinson Effect of Deletion or Overexpression of the 19-Kilodalton Lipoprotein Rv3763 on the Innate Response to Mycobacterium tuberculosis Infect. Immun., October 1, 2005; 73(10): 6831 - 6837. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Bergman, L. A. Cummings, S. L. R. Barrett, K. D. Smith, J. C. Lara, A. Aderem, and B. T. Cookson CD4+ T Cells and Toll-Like Receptors Recognize Salmonella Antigens Expressed in Bacterial Surface Organelles Infect. Immun., March 1, 2005; 73(3): 1350 - 1356. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. J. Cheadle, D. O'Donnell, P. J. Selby, and A. M. Jackson Closely Related Mycobacterial Strains Demonstrate Contrasting Levels of Efficacy as Antitumor Vaccines and Are Processed for Major Histocompatibility Complex Class I Presentation by Multiple Routes in Dendritic Cells Infect. Immun., February 1, 2005; 73(2): 784 - 794. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. K. Pai, M. E. Pennini, A. A. R. Tobian, D. H. Canaday, W. H. Boom, and C. V. Harding Prolonged Toll-Like Receptor Signaling by Mycobacterium tuberculosis and Its 19-Kilodalton Lipoprotein Inhibits Gamma Interferon-Induced Regulation of Selected Genes in Macrophages Infect. Immun., November 1, 2004; 72(11): 6603 - 6614. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Romano, O. Denis, S. D'Souza, X.-M. Wang, T. H. M. Ottenhoff, J.-M. Brulet, and K. Huygen Induction of In Vivo Functional Db-Restricted Cytolytic T Cell Activity against a Putative Phosphate Transport Receptor of Mycobacterium tuberculosis J. Immunol., June 1, 2004; 172(11): 6913 - 6921. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Hmama, K. Sendide, A. Talal, R. Garcia, K. Dobos, and N. E. Reiner Quantitative analysis of phagolysosome fusion in intact cells: inhibition by mycobacterial lipoarabinomannan and rescue by an 1{alpha},25-dihydroxyvitamin D3-phosphoinositide 3-kinase pathway J. Cell Sci., April 15, 2004; 117(10): 2131 - 2140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Shams, P. F. Barnes, S. E. Weis, P. Klucar, and B. Wizel Human CD8+ T cells recognize epitopes of the 28-kDa hemolysin and the 38-kDa antigen of Mycobacterium tuberculosis J. Leukoc. Biol., December 1, 2003; 74(6): 1008 - 1014. [Abstract] [Full Text]
|
|
|
|
|
|
A. J. Gehring, R. E. Rojas, D. H. Canaday, D. L. Lakey, C. V. Harding, and W. H. Boom The Mycobacterium tuberculosis 19-Kilodalton Lipoprotein Inhibits Gamma Interferon-Regulated HLA-DR and Fc{gamma}R1 on Human Macrophages through Toll-Like Receptor 2 Infect. Immun., August 1, 2003; 71(8): 4487 - 4497. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. K. Pai, M. Convery, T. A. Hamilton, W. H. Boom, and C. V. Harding Inhibition of IFN-{gamma}-Induced Class II Transactivator Expression by a 19-kDa Lipoprotein from Mycobacterium tuberculosis: A Potential Mechanism for Immune Evasion J. Immunol., July 1, 2003; 171(1): 175 - 184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.-H. Hovav, J. Mullerad, L. Davidovitch, Y. Fishman, F. Bigi, A. Cataldi, and H. Bercovier The Mycobacterium tuberculosis Recombinant 27-Kilodalton Lipoprotein Induces a Strong Th1-Type Immune Response Deleterious to Protection Infect. Immun., June 1, 2003; 71(6): 3146 - 3154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Tailleux, O. Neyrolles, S. Honore-Bouakline, E. Perret, F. Sanchez, J.-P. Abastado, P. H. Lagrange, J. C. Gluckman, M. Rosenzwajg, and J.-L. Herrmann Constrained Intracellular Survival of Mycobacterium tuberculosis in Human Dendritic Cells J. Immunol., February 15, 2003; 170(4): 1939 - 1948. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. S. Heinzel, J. E. Grotzke, R. A. Lines, D. A. Lewinsohn, A. L. McNabb, D. N. Streblow, V. M. Braud, H. J. Grieser, J. T. Belisle, and D. M. Lewinsohn HLA-E-dependent Presentation of Mtb-derived Antigen to Human CD8+ T Cells J. Exp. Med., December 2, 2002; 196(11): 1473 - 1481. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. G. Russell, H. C. Mwandumba, and E. E. Rhoades Mycobacterium and the coat of many lipids J. Cell Biol., August 5, 2002; 158(3): 421 - 426. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D'Souza, V. Rosseels, O. Denis, A. Tanghe, N. De Smet, F. Jurion, K. Palfliet, N. Castiglioni, A. Vanonckelen, C. Wheeler, et al. Improved Tuberculosis DNA Vaccines by Formulation in Cationic Lipids Infect. Immun., July 1, 2002; 70(7): 3681 - 3688. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Grode, M. Kursar, J. Fensterle, S. H. E. Kaufmann, and J. Hess Cell-Mediated Immunity Induced by Recombinant Mycobacterium bovis Bacille Calmette-Guerin Strains Against an Intracellular Bacterial Pathogen: Importance of Antigen Secretion or Membrane-Targeted Antigen Display as Lipoprotein for Vaccine Efficacy J. Immunol., February 15, 2002; 168(4): 1869 - 1876. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Neufert, R. K. Pai, E. H. Noss, M. Berger, W. H. Boom, and C. V. Harding Mycobacterium tuberculosis 19-kDa Lipoprotein Promotes Neutrophil Activation J. Immunol., August 1, 2001; 167(3): 1542 - 1549. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. A. Post, C. Manca, O. Neyrolles, B. Ryffel, D. B. Young, and G. Kaplan Mycobacterium tuberculosis 19-Kilodalton Lipoprotein Inhibits Mycobacterium smegmatis-Induced Cytokine Production by Human Macrophages In Vitro Infect. Immun., March 1, 2001; 69(3): 1433 - 1439. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
