| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Department of Immunology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan;
Division of Environmental Pharmacology, Department of Pharmaceutical Sciences, Osaka University, Osaka, Japan; and
Department of Molecular Immunology, Nara Institute for Science and Technology, Ikoma, Japan
A cDNA encoding a membrane-associated complement (C) regulatory protein was identified here for the first time in an oviparous vertebrate, chicken. This protein, named Cremp, possessed five short consensus repeats (SCRs) and one SCR-like domain followed by a transmembrane domain and a cytoplasmic tail. SCR1/SCR2 of Cremp were 43.6% identical with SCR2/SCR3 of human decay-accelerating factor (CD55), and SCR3/SCR4 were 45.3% identical with those of human membrane cofactor protein (CD46). Cremp is likely to be an ancestral hybrid protein of human decay-accelerating factor and membrane cofactor protein rather than a homolog of rodent C receptor 1-related protein y, which structurally resembles human CR1 (CD35). Chinese hamster ovary cells transfected with Cremp were efficiently protected from chicken C but not from human or rabbit C in both classical and alternative pathways. Thus, chicken Cremp is a membrane C regulator for cell protection against homologous C. Cremp mRNA was seen as a doublet comprised of a faint band of 2.2 kb and a thick band of 3.0 kb on RNA blotting analysis. An Ab against chicken Cremp recognized a single band of 46.8 kDa on immunoblotting. mRNA and protein of Cremp were ubiquitously expressed in all chicken organs tested. Minute amounts of dimer were present in some tissues. Surface expression of Cremp was confirmed by flow cytometry and immunofluorescence analysis. These results suggested that even in nonmammals a C regulatory membrane protein with ubiquitous tissue distribution should be a prerequisite for protection of host cells from homologous C attack.
This article has been cited by other articles:
|
|
 |
|
  T. Ebihara, H. Masuda, T. Akazawa, M. Shingai, H. Kikuta, T. Ariga, M. Matsumoto, and T. Seya Induction of NKG2D ligands on human dendritic cells by TLR ligand stimulation and RNA virus infection Int. Immunol., October 1, 2007; 19(10): 1145 - 1155. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ishii, A. Matsuo, H. Sawa, T. Tsujita, K. Shida, M. Matsumoto, and T. Seya Lamprey TLRs with Properties Distinct from Those of the Variable Lymphocyte Receptors J. Immunol., January 1, 2007; 178(1): 397 - 406. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Seya, K. Funami, M. Taniguchi, and M. Matsumoto Antibodies against human Toll-like receptors (TLRs): TLR distribution and localization in human dendritic cells Innate Immunity, December 1, 2005; 11(6): 369 - 374. [Abstract] [PDF]
|
|
|
|
|
|
H. Oshiumi, K. Shida, R. Goitsuka, Y. Kimura, J. Katoh, S. Ohba, Y. Tamaki, T. Hattori, N. Yamada, N. Inoue, et al. Regulator of Complement Activation (RCA) Locus in Chicken: Identification of Chicken RCA Gene Cluster and Functional RCA Proteins J. Immunol., August 1, 2005; 175(3): 1724 - 1734. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kimura, N. Inoue, A. Fukui, H. Oshiumi, M. Matsumoto, M. Nonaka, S. Kuratani, T. Fujita, M. Nonaka, and T. Seya A Short Consensus Repeat-Containing Complement Regulatory Protein of Lamprey That Participates in Cleavage of Lamprey Complement 3 J. Immunol., July 15, 2004; 173(2): 1118 - 1128. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Inoue, M. Ikawa, T. Nakanishi, M. Matsumoto, M. Nomura, T. Seya, and M. Okabe Disruption of Mouse CD46 Causes an Accelerated Spontaneous Acrosome Reaction in Sperm Mol. Cell. Biol., April 1, 2003; 23(7): 2614 - 2622. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Fukui, N. Inoue, M. Matsumoto, M. Nomura, K. Yamada, Y. Matsuda, K. Toyoshima, and T. Seya Molecular Cloning and Functional Characterization of Chicken Toll-like Receptors. A SINGLE CHICKEN TOLL COVERS MULTIPLE MOLECULAR PATTERNS J. Biol. Chem., December 7, 2001; 276(50): 47143 - 47149. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tsuji, J. Uehori, M. Matsumoto, Y. Suzuki, A. Matsuhisa, K. Toyoshima, and T. Seya Human Intelectin Is a Novel Soluble Lectin That Recognizes Galactofuranose in Carbohydrate Chains of Bacterial Cell Wall J. Biol. Chem., June 22, 2001; 276(26): 23456 - 23463. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
