The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Girschick, H. J.
Right arrow Articles by Lipsky, P. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Girschick, H. J.
Right arrow Articles by Lipsky, P. E.
The Journal of Immunology, 2001, 166: 377-386.
Copyright © 2001 by The American Association of Immunologists

RAG1 and RAG2 Expression by B Cell Subsets from Human Tonsil and Peripheral Blood1

Hermann J. Girschick, Amrie C. Grammer2, Toshihiro Nanki2, Marlyn Mayo and Peter E. Lipsky2,3

Department of Internal Medicine, Harold C. Simmons Arthritis Research Center, University of Texas Southwestern Medical Center, Dallas, TX 75235

It has been suggested that B cells acquire the capacity for secondary V(D)J recombination during germinal center (GC) reactions. The nature of these B cells remains controversial. Subsets of tonsil and blood B cells and also individual B cells were examined for the expression of recombination-activating gene (RAG) mRNA. Semiquantitative analysis indicated that RAG1 mRNA was present in all tonsil B cell subsets, with the largest amount found in naive B cells. RAG2 mRNA was only found in tonsil naive B cells, centrocytes, and to a lesser extent in centroblasts. Neither RAG1 nor RAG2 mRNA was routinely found in normal peripheral blood B cells. In individual tonsil B cells, RAG1 and RAG2 mRNAs were found in 18% of naive B cells, 22% of GC founder cells, 0% of centroblasts, 13% of centrocytes, and 9% of memory B cells. Individual naive tonsil B cells containing both RAG1 and RAG2 mRNA were activated (CD69+). In normal peripheral blood ~5% of B cells expressed both RAG1 and RAG2. These cells were uniformly postswitch memory B cells as documented by the coexpression of IgG mRNA. These results indicate that coordinate RAG expression is not found in normal peripheral naive B cells but is up-regulated in naive B cells which are activated in the tonsil. With the exception of centroblasts, RAG1 and RAG2 expression can be found in all components of the GC, including postswitch memory B cells, some of which may circulate in the blood of normal subjects.




This article has been cited by other articles:


Home page
 J. Immunol.Home page
W. Su, J. N. Gordon, F. Barone, L. Boursier, W. Turnbull, S. Mendis, D. K. Dunn-Walters, and J. Spencer
Lambda Light Chain Revision in the Human Intestinal IgA Response
J. Immunol., July 15, 2008; 181(2): 1264 - 1271.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Hillion, M. Dueymes, P. Youinou, and C. Jamin
IL-6 Contributes to the Expression of RAGs in Human Mature B Cells
J. Immunol., November 15, 2007; 179(10): 6790 - 6798.
[Abstract] [Full Text] [PDF]

Home page
 Ann Rheum DisHome page
C Faber, H Morbach, S K Singh, and H J Girschick
Differential expression patterns of recombination-activating genes in individual mature B cells in juvenile idiopathic arthritis
Ann Rheum Dis, October 1, 2006; 65(10): 1351 - 1356.
[Abstract] [Full Text] [PDF]

Home page
 Ann Rheum DisHome page
H Morbach, S K Singh, C Faber, P E Lipsky, and H J Girschick
Analysis of RAG expression by peripheral blood CD5+ and CD5- B cells of patients with childhood systemic lupus erythematosus
Ann Rheum Dis, April 1, 2006; 65(4): 482 - 487.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Hillion, A. Saraux, P. Youinou, and C. Jamin
Expression of RAGs in Peripheral B Cells outside Germinal Centers Is Associated with the Expression of CD5
J. Immunol., May 1, 2005; 174(9): 5553 - 5561.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. S. Rice, J. Newman, C. Wang, D. J. Michael, and B. Diamond
Receptor editing in peripheral B cell tolerance
PNAS, February 1, 2005; 102(5): 1608 - 1613.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T.-H. Thai and J. F. Kearney
Distinct and Opposite Activities of Human Terminal Deoxynucleotidyltransferase Splice Variants
J. Immunol., September 15, 2004; 173(6): 4009 - 4019.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. H. Sasso, M. Martinez, S. L. Yarfitz, P. Ghillani, L. Musset, J.-C. Piette, and P. Cacoub
Frequent Joining of Bcl-2 to a JH6 Gene in Hepatitis C Virus-Associated t(14;18)
J. Immunol., September 1, 2004; 173(5): 3549 - 3556.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
W. Su, L. Boursier, A. Padala, J. D. Sanderson, and J. Spencer
Biases in Ig {lambda} Light Chain Rearrangements in Human Intestinal Plasma Cells
J. Immunol., February 15, 2004; 172(4): 2360 - 2366.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
N. Meru, A. Jung, I. Baumann, and G. Niedobitek
Expression of the recombination-activating genes in extrafollicular lymphocytes but no apparent reinduction in germinal center reactions in human tonsils
Blood, January 15, 2002; 99(2): 531 - 537.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2001 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2001 by The American Association of Immunologists, Inc. All rights reserved.