| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
CUTTING EDGE |
Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Tokyo, Japan
MD-2 associates with the extracellular domain of Toll-like receptor 4 (TLR4) and greatly enhances LPS signaling via TLR4. Taxol, which mimics the action of LPS on murine macrophages, induces signals via mouse TLR4-MD-2, but not via human TLR4-MD-2. Here we investigated the molecular basis for this species-specific action of Taxol. Expression of mouse MD-2 conferred both LPS and Taxol responsiveness on human embryonic kidney 293 cells expressing mouse TLR4, whereas expression of human MD-2 conferred LPS responsiveness alone, suggesting that MD-2 is responsible for the species-specificity as to Taxol responsiveness. Furthermore, mouse MD-2 mutants, in which Gln22 was changed to other amino acids, showed dramatically reduced ability to confer Taxol responsiveness, although their ability to confer LPS responsiveness was not affected. These results indicated that Gln22 of mouse MD-2 is essential for Taxol signaling but not for LPS signaling.
This article has been cited by other articles:
|
|
 |
|
  S. M. Zimmer, J. Liu, J. L. Clayton, D. S. Stephens, and J. P. Snyder Paclitaxel Binding to Human and Murine MD-2 J. Biol. Chem., October 10, 2008; 283(41): 27916 - 27926. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. D. Snyder, R. E. Oberley-Deegan, K. L. Goss, S. A. Romig-Martin, L. L. Stoll, J. M. Snyder, and N. L. Weintraub Surfactant protein D is expressed and modulates inflammatory responses in human coronary artery smooth muscle cells Am J Physiol Heart Circ Physiol, May 1, 2008; 294(5): H2053 - H2059. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Rallabhandi, A. Awomoyi, K. E. Thomas, A. Phalipon, Y. Fujimoto, K. Fukase, S. Kusumoto, N. Qureshi, M. B. Sztein, and S. N. Vogel Differential Activation of Human TLR4 by Escherichia coli and Shigella flexneri 2a Lipopolysaccharide: Combined Effects of Lipid A Acylation State and TLR4 Polymorphisms on Signaling J. Immunol., January 15, 2008; 180(2): 1139 - 1147. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M Zimmer, S. M Zughaier, Y.-L. Tzeng, and D. S Stephens Human MD-2 discrimination of meningococcal lipid A structures and activation of TLR4 Glycobiology, August 1, 2007; 17(8): 847 - 856. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Keestra, M. R. de Zoete, R. A. M. H. van Aubel, and J. P. M. van Putten The Central Leucine-Rich Repeat Region of Chicken TLR16 Dictates Unique Ligand Specificity and Species-Specific Interaction with TLR2 J. Immunol., June 1, 2007; 178(11): 7110 - 7119. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. M. Doherty, P. K. Shah, M. Arditi, L. L. Stoll, G. M. Denning, and N. L. Weintraub Lipopolysaccharide, Toll-Like Receptors, and the Immune Contribution to Atherosclerosis Arterioscler. Thromb. Vasc. Biol., May 1, 2005; 25(5): e38 - e39. [Full Text] [PDF]
|
|
|
|
 |
|
 J. Pugin, S. Stern-Voeffray, B. Daubeuf, M. A. Matthay, G. Elson, and I. Dunn-Siegrist Soluble MD-2 activity in plasma from patients with severe sepsis and septic shock Blood, December 15, 2004; 104(13): 4071 - 4079. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kawasaki, R. K. Ernst, and S. I. Miller Deacylation and palmitoylation of lipid A by Salmonellae outer membrane enzymes modulate host signaling through Toll-like receptor 4 Innate Immunity, December 1, 2004; 10(6): 439 - 444. [Abstract] [PDF]
|
|
|
|
|
|
L. L. Stoll, G. M. Denning, and N. L. Weintraub Potential Role of Endotoxin as a Proinflammatory Mediator of Atherosclerosis Arterioscler. Thromb. Vasc. Biol., December 1, 2004; 24(12): 2227 - 2236. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Fujimoto, S. Yamazaki, A. Eto-Kimura, K. Takeshige, and T. Muta The Amino-terminal Region of Toll-like Receptor 4 Is Essential for Binding to MD-2 and Receptor Translocation to the Cell Surface J. Biol. Chem., November 12, 2004; 279(46): 47431 - 47437. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Gon, Y. Asai, S. Hashimoto, K. Mizumura, I. Jibiki, T. Machino, C. Ra, and T. Horie A20 Inhibits Toll-Like Receptor 2- and 4-Mediated Interleukin-8 Synthesis in Airway Epithelial Cells Am. J. Respir. Cell Mol. Biol., September 1, 2004; 31(3): 330 - 336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. N. Kennedy, G. E. D. Mullen, C. A. Leifer, C. Lee, A. Mazzoni, K. N. Dileepan, and D. M. Segal A Complex of Soluble MD-2 and Lipopolysaccharide Serves as an Activating Ligand for Toll-like Receptor 4 J. Biol. Chem., August 13, 2004; 279(33): 34698 - 34704. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-i. Saitoh, S. Akashi, T. Yamada, N. Tanimura, M. Kobayashi, K. Konno, F. Matsumoto, K. Fukase, S. Kusumoto, Y. Nagai, et al. Lipid A antagonist, lipid IVa, is distinct from lipid A in interaction with Toll-like receptor 4 (TLR4)-MD-2 and ligand-induced TLR4 oligomerization Int. Immunol., July 1, 2004; 16(7): 961 - 969. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kawasaki, R. K. Ernst, and S. I. Miller 3-O-Deacylation of Lipid A by PagL, a PhoP/PhoQ-regulated Deacylase of Salmonella typhimurium, Modulates Signaling through Toll-like Receptor 4 J. Biol. Chem., May 7, 2004; 279(19): 20044 - 20048. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Oshiumi, M. Sasai, K. Shida, T. Fujita, M. Matsumoto, and T. Seya TIR-containing Adapter Molecule (TICAM)-2, a Bridging Adapter Recruiting to Toll-like Receptor 4 TICAM-1 That Induces Interferon-{beta} J. Biol. Chem., December 12, 2003; 278(50): 49751 - 49762. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Re and J. L. Strominger Separate Functional Domains of Human MD-2 Mediate Toll-Like Receptor 4-Binding and Lipopolysaccharide Responsiveness J. Immunol., November 15, 2003; 171(10): 5272 - 5276. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Pinhal-Enfield, M. Ramanathan, G. Hasko, S. N. Vogel, A. L. Salzman, G.-J. Boons, and S. J. Leibovich An Angiogenic Switch in Macrophages Involving Synergy between Toll-Like Receptors 2, 4, 7, and 9 and Adenosine A2A Receptors Am. J. Pathol., August 1, 2003; 163(2): 711 - 721. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kawasaki, H. Nogawa, and M. Nishijima Identification of Mouse MD-2 Residues Important for Forming the Cell Surface TLR4-MD-2 Complex Recognized by Anti-TLR4-MD-2 Antibodies, and for Conferring LPS and Taxol Responsiveness on Mouse TLR4 by Alanine-Scanning Mutagenesis J. Immunol., January 1, 2003; 170(1): 413 - 420. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Muroi, T. Ohnishi, and K.-i. Tanamoto MD-2, a Novel Accessory Molecule, Is Involved in Species-Specific Actions of Salmonella Lipid A Infect. Immun., July 1, 2002; 70(7): 3546 - 3550. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Re and J. L. Strominger Monomeric Recombinant MD-2 Binds Toll-like Receptor 4 Tightly and Confers Lipopolysaccharide Responsiveness J. Biol. Chem., June 21, 2002; 277(26): 23427 - 23432. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M A Moscarello, B Mak, T A Nguyen, D D Wood, F Mastronardi, and S K Ludwin Paclitaxel (Taxol) attenuates clinical disease in a spontaneously demyelinating transgenic mouse and induces remyelination Multiple Sclerosis, April 1, 2002; 8(2): 130 - 138. [Abstract] [PDF]
|
|
|
|
|
|
K. Gomi, K. Kawasaki, Y. Kawai, M. Shiozaki, and M. Nishijima Toll-Like Receptor 4-MD-2 Complex Mediates the Signal Transduction Induced by Flavolipin, an Amino Acid-Containing Lipid Unique to Flavobacterium meningosepticum J. Immunol., March 15, 2002; 168(6): 2939 - 2943. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H. Diks, S. J.H. van Deventer, and M. P. Peppelenbosch Invited review: Lipopolysaccharide recognition, internalisation, signalling and other cellular effects Innate Immunity, October 1, 2001; 7(5): 335 - 348. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
