| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Dipartimento di Medicina Sperimentale e Clinica, Università di Catanzaro Magna Graecia, Catanzaro, Italy;
Department of Pediatric Gastroenterology, St. Bartholomew’s and Royal London School of Medicine and Dentistry, London, United Kingdom;
Cattedra di Gastroenterologia, Seconda Università di Napoli, Naples, Italy;
§
Dipartimento di Biologia e Patologia Cellulare e Molecolare, Università di Napoli Federico II, Naples, Italy; and
¶
Università di Roma Tor Vergata, Rome, Italy
Helicobacter pylori (Hp)-associated gastritis is characterized by an increased number of acute and chronic inflammatory cells secreting cytokines that contribute to maintain and expand the local inflammation. Locally induced IL-8 is believed to play a major role in the Hp-associated acute inflammatory response. Factors/mechanisms that regulate IL-8 induction are, however, not fully understood. In the present study we investigated whether Hp infection is associated with an increased production of IL-17, a T cell-derived cytokine capable of modulating IL-8 gene expression. We showed that both IL-17 RNA transcripts and protein were expressed at a higher level in the whole gastric mucosal and lamina propria mononuclear cell samples from Hp-infected patients than in those from uninfected subjects. Hp eradication was associated with a marked down-regulation of IL-17 expression. The addition of a neutralizing anti-IL-17 Ab to the gastric lamina propria mononuclear cell cultures resulted in a significant inhibition of IL-8 secretion, indicating that IL-17 contributes to enhance IL-8 in the Hp-colonized gastric mucosa. Consistently, stimulation of MKN 28 cells, a gastric epithelial cell line, with IL-17 increased IL-8 secretion. Finally, conditioned medium from the IL-17-stimulated MKN 28 cell cultures promoted the in vitro polymorphonuclear leukocyte migration. This effect was inhibitable by a neutralizing IL-8 but not IL-17 Ab. Together, these data indicate that biologically active IL-17 production is increased during Hp infection, suggesting the possibility that this cytokine may play an important role in the inflammatory response to the Hp colonization.
This article has been cited by other articles:
|
|
 |
|
  S. Shahrara, S. R. Pickens, A. Dorfleutner, and R. M. Pope IL-17 Induces Monocyte Migration in Rheumatoid Arthritis J. Immunol., March 15, 2009; 182(6): 3884 - 3891. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Webb, A. Johnson, M. Fortunato, A. Platt, T. Crabbe, M. I. Christie, G. F. Watt, S. G. Ward, and L. A. Jopling Evidence for PI-3K-dependent migration of Th17-polarized cells in response to CCR2 and CCR6 agonists J. Leukoc. Biol., October 1, 2008; 84(4): 1202 - 1212. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M J Blaser, Y Chen, and J Reibman Authors' response Gut, August 1, 2008; 57(8): 1179 - 1179. [Full Text] [PDF]
|
|
|
|
|
|
K. Yamauchi, I.-J. Choi, H. Lu, H. Ogiwara, D. Y. Graham, and Y. Yamaoka Regulation of IL-18 in Helicobacter pylori Infection J. Immunol., January 15, 2008; 180(2): 1207 - 1216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Niess, F. Leithauser, G. Adler, and J. Reimann Commensal Gut Flora Drives the Expansion of Proinflammatory CD4 T Cells in the Colonic Lamina Propria under Normal and Inflammatory Conditions J. Immunol., January 1, 2008; 180(1): 559 - 568. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Maitra, F. Shen, W. Hanel, K. Mossman, J. Tocker, D. Swart, and S. L. Gaffen Distinct functional motifs within the IL-17 receptor regulate signal transduction and target gene expression PNAS, May 1, 2007; 104(18): 7506 - 7511. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. N. Mathur, H.-C. Chang, D. G. Zisoulis, R. Kapur, M. L. Belladonna, G. S. Kansas, and M. H. Kaplan T-bet is a critical determinant in the instability of the IL-17-secreting T-helper phenotype Blood, September 1, 2006; 108(5): 1595 - 1601. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. K. Liu, X. Lin, and S. L. Gaffen Crucial Role for Nuclear Factor of Activated T Cells in T Cell Receptor-mediated Regulation of Human Interleukin-17 J. Biol. Chem., December 10, 2004; 279(50): 52762 - 52771. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Sebkova, A. Pellicano, G. Monteleone, B. Grazioli, G. Guarnieri, M. Imeneo, F. Pallone, and F. Luzza Extracellular Signal-Regulated Protein Kinase Mediates Interleukin 17 (IL-17)-Induced IL-8 Secretion in Helicobacter pylori-Infected Human Gastric Epithelial Cells Infect. Immun., September 1, 2004; 72(9): 5019 - 5026. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kranzer, A. Eckhardt, M. Aigner, G. Knoll, L. Deml, C. Speth, N. Lehn, M. Rehli, and W. Schneider-Brachert Induction of Maturation and Cytokine Release of Human Dendritic Cells by Helicobacter pylori Infect. Immun., August 1, 2004; 72(8): 4416 - 4423. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Ruddy, F. Shen, J. B. Smith, A. Sharma, and S. L. Gaffen Interleukin-17 regulates expression of the CXC chemokine LIX/CXCL5 in osteoblasts: implications for inflammation and neutrophil recruitment J. Leukoc. Biol., July 1, 2004; 76(1): 135 - 144. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S Fujino, A Andoh, S Bamba, A Ogawa, K Hata, Y Araki, T Bamba, and Y Fujiyama Increased expression of interleukin 17 in inflammatory bowel disease Gut, January 1, 2003; 52(1): 65 - 70. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Maertzdorf, A. D. M. E. Osterhaus, and G. M. G. M. Verjans IL-17 Expression in Human Herpetic Stromal Keratitis: Modulatory Effects on Chemokine Production by Corneal Fibroblasts J. Immunol., November 15, 2002; 169(10): 5897 - 5903. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Aggarwal and A. L. Gurney IL-17: prototype member of an emerging cytokine family J. Leukoc. Biol., January 1, 2002; 71(1): 1 - 8. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Cox, C. L. Clayton, T. Tomita, D. M. Wallace, P. A. Robinson, and J. E. Crabtree cDNA Array Analysis of cag Pathogenicity Island-Associated Helicobacter pylori Epithelial Cell Response Genes Infect. Immun., November 1, 2001; 69(11): 6970 - 6980. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Akkoyunlu, S. E. Malawista, J. Anguita, and E. Fikrig Exploitation of Interleukin-8-Induced Neutrophil Chemotaxis by the Agent of Human Granulocytic Ehrlichiosis Infect. Immun., September 1, 2001; 69(9): 5577 - 5588. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
