The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tan-Pertel, H. T.
Right arrow Articles by Gasson, J. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tan-Pertel, H. T.
Right arrow Articles by Gasson, J. C.
The Journal of Immunology, 2000, 165: 4428-4436.
Copyright © 2000 by The American Association of Immunologists

Notch Signaling Enhances Survival and Alters Differentiation of 32D Myeloblasts1

Hongying Tina Tan-Pertel*, Liberty Walker{dagger}, Damaris Browning*, Alison Miyamoto{dagger}, Gerry Weinmaster{dagger} and Judith C. Gasson2,*,{dagger}

* Division of Hematology-Oncology, Department of Medicine, University of California at Los Angeles School of Medicine and Jonsson Comprehensive Cancer Center, Los Angeles, CA 90095; and {dagger} Department of Biological Chemistry, University of California at Los Angeles Molecular Biology Institute, Los Angeles, CA 90095

The Notch transmembrane receptors play important roles in precursor survival and cell fate specification during hematopoiesis. To investigate the function of Notch and the signaling events activated by Notch in myeloid development, we expressed truncated forms of Notch1 or Notch2 proteins that either can or cannot activate the core binding factor 1 (CBF1) in 32D (clone 3) myeloblasts. 32D cells proliferate as blasts in the presence of the cytokines, GM-CSF or IL-3, but they initiate differentiation and undergo granulopoiesis in the presence of granulocyte CSF (G-CSF). 32D cells expressing constitutively active forms of Notch1 or Notch2 proteins that signal through the CBF1 pathway maintained significantly higher numbers of viable cells and exhibited less cell death during G-CSF induction compared with controls. They also displayed enhanced entry into granulopoiesis, and inhibited postmitotic terminal differentiation. In contrast, Notch1 constructs that either lacked sequences necessary for CBF1 binding or that failed to localize to the nucleus had little effect. Elevated numbers of viable cells during G-CSF treatment were also observed in 32D cells overexpressing the basic helix-loop-helix protein (bHLH), HES1, consistent with activation of the CBF1 pathway. Taken together, our data suggest that Notch signaling enhances 32D cell survival, promotes entry into granulopoiesis, and inhibits postmitotic differentiation through a CBF1-dependent pathway.




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
C. Wang, R. Qi, N. Li, Z. Wang, H. An, Q. Zhang, Y. Yu, and X. Cao
Notch1 Signaling Sensitizes Tumor Necrosis Factor-related Apoptosis-inducing Ligand-induced Apoptosis in Human Hepatocellular Carcinoma Cells by Inhibiting Akt/Hdm2-mediated p53 Degradation and Up-regulating p53-dependent DR5 Expression
J. Biol. Chem., June 12, 2009; 284(24): 16183 - 16190.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
L. Zhou, L. W. Li, Q. Yan, B. Petryniak, Y. Man, C. Su, J. Shim, S. Chervin, and J. B. Lowe
Notch-dependent control of myelopoiesis is regulated by fucosylation
Blood, July 15, 2008; 112(2): 308 - 319.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Sakata-Yanagimoto, E. Nakagami-Yamaguchi, T. Saito, K. Kumano, K. Yasutomo, S. Ogawa, M. Kurokawa, and S. Chiba
From the Cover: Coordinated regulation of transcription factors through Notch2 is an important mediator of mast cell fate
PNAS, June 3, 2008; 105(22): 7839 - 7844.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
U. Ganapati, H. T. Tan, M. Lynch, M. Dolezal, S. de Vos, and J. C. Gasson
Modeling Notch Signaling in Normal and Neoplastic Hematopoiesis: Global Gene Expression Profiling in Response to Activated Notch Expression
Stem Cells, August 1, 2007; 25(8): 1872 - 1880.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. F. de Pooter, T. M. Schmitt, J. L. de la Pompa, Y. Fujiwara, S. H. Orkin, and J. C. Zuniga-Pflucker
Notch Signaling Requires GATA-2 to Inhibit Myelopoiesis from Embryonic Stem Cells and Primary Hemopoietic Progenitors
J. Immunol., May 1, 2006; 176(9): 5267 - 5275.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
H. Neves, F. Weerkamp, A. C. Gomes, B. A. E. Naber, P. Gameiro, J. D. Becker, P. Lucio, N. Clode, J. J. M. Van Dongen, F. J. T. Staal, et al.
Effects of Delta1 and Jagged1 on Early Human Hematopoiesis: Correlation with Expression of Notch Signaling-Related Genes in CD34+ Cells
Stem Cells, May 1, 2006; 24(5): 1328 - 1337.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Miyamoto, R. Lau, P. W. Hein, J. M. Shipley, and G. Weinmaster
Microfibrillar Proteins MAGP-1 and MAGP-2 Induce Notch1 Extracellular Domain Dissociation and Receptor Activation
J. Biol. Chem., April 14, 2006; 281(15): 10089 - 10097.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Ishiko, I. Matsumura, S. Ezoe, K. Gale, J. Ishiko, Y. Satoh, H. Tanaka, H. Shibayama, M. Mizuki, T. Era, et al.
Notch Signals Inhibit the Development of Erythroid/Megakaryocytic Cells by Suppressing GATA-1 Activity through the Induction of HES1
J. Biol. Chem., February 11, 2005; 280(6): 4929 - 4939.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
S. J. Erkeland, M. Valkhof, C. Heijmans-Antonissen, A. van Hoven-Beijen, R. Delwel, M. H. A. Hermans, and I. P. Touw
Large-Scale Identification of Disease Genes Involved in Acute Myeloid Leukemia
J. Virol., February 15, 2004; 78(4): 1971 - 1980.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Z. Duan, F.-Q. Li, J. Wechsler, K. Meade-White, K. Williams, K. F. Benson, and M. Horwitz
A Novel Notch Protein, N2N, Targeted by Neutrophil Elastase and Implicated in Hereditary Neutropenia
Mol. Cell. Biol., January 1, 2004; 24(1): 58 - 70.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. Schroeder, H. Kohlhof, N. Rieber, and U. Just
Notch Signaling Induces Multilineage Myeloid Differentiation and Up-Regulates PU.1 Expression
J. Immunol., June 1, 2003; 170(11): 5538 - 5548.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
T. Yamada, H. Yamazaki, T. Yamane, M. Yoshino, H. Okuyama, M. Tsuneto, T. Kurino, S.-I. Hayashi, and S. Sakano
Regulation of osteoclast development by Notch signaling directed to osteoclast precursors and through stromal cells
Blood, March 15, 2003; 101(6): 2227 - 2234.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Weijzen, M. P. Velders, A. G. Elmishad, P. E. Bacon, J. R. Panella, B. J. Nickoloff, L. Miele, and W. M. Kast
The Notch Ligand Jagged-1 Is Able to Induce Maturation of Monocyte-Derived Human Dendritic Cells
J. Immunol., October 15, 2002; 169(8): 4273 - 4278.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
L. Walker, A. Carlson, H. T. Tan-Pertel, G. Weinmaster, and J. Gasson
The Notch Receptor and Its Ligands Are Selectively Expressed During Hematopoietic Development in the Mouse
Stem Cells, November 1, 2001; 19(6): 543 - 552.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. K. Hadland, N. R. Manley, D.-m. Su, G. D. Longmore, C. L. Moore, M. S. Wolfe, E. H. Schroeter, and R. Kopan
gamma -Secretase inhibitors repress thymocyte development
PNAS, June 19, 2001; 98(13): 7487 - 7491.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Ingles-Esteve, L. Espinosa, L. A. Milner, C. Caelles, and A. Bigas
Phosphorylation of Ser2078 Modulates the Notch2 Function in 32D Cell Differentiation
J. Biol. Chem., November 21, 2001; 276(48): 44873 - 44880.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2000 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2000 by The American Association of Immunologists, Inc. All rights reserved.