| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Department of Experimental Medicine, Human Anatomy Section, University of Genova,
Monoclonal Antibody Unit, National Institute for Cancer Research, Genova, Italy;
Department of Biomorphology, Human Anatomy Section, University of Messina, Messina, Italy; and
§
Immunex, Seattle, WA
The inhibitory molecule CD85/LIR-1/ILT2 has been detected previously on the surface of a small proportion of T lymphocytes. In this study, evidence is provided that, although only a fraction of CD3+ cells are stained by mAb specific for CD85/LIR-1/ILT2 on their surface, this inhibitory receptor is present in the cytoplasm of all T lymphocytes, and that it is detectable on the surface of all T cell clones by the M402 mAb. Biochemical analyses further demonstrate that CD85/LIR-1/ILT2 is present in all T clones analyzed, and that the protein is tyrosine-phosphorylated. Expression of mRNA coding for CD85/LIR-1/ILT2 has been assessed by RT-PCR. Notably, in the NKL cell line and in one T cell clone, amplification of the messenger required 30 cycles only, whereas, in other T cell clones, an amplification product was detected by increasing the number of cycles. CD85/LIR-1/ILT2 inhibits CD3/TCR-mediated activation in both CD4+ and CD8+ clones, and it down-regulates Ag recognition by CD8+ cells in a clonally distributed fashion. Addition of anti-ILT2 HP-F1 mAb in the cytolytic assay enhances target cell lysis mediated by Ag-specific CTL. This could be due to interference of the mAb with receptor/ligand interactions. In contrast, HP-F1 mAb cross-linking triggers inhibitory signals that reduce cytotoxicity. CD85/LIR-1/ILT2 also controls responses to recall Ags and, in low responders, its engagement sharply increases T cell proliferation. The inhibitory function of the molecule is also confirmed by its ability to reduce CD3/TCR-induced intracellular Ca2+ mobilization.
This article has been cited by other articles:
|
|
 |
|
  Y.-H. Huang, A. L. Zozulya, C. Weidenfeller, N. Schwab, and H. Wiendl T cell suppression by naturally occurring HLA-G-expressing regulatory CD4+ T cells is IL-10-dependent and reversible J. Leukoc. Biol., August 1, 2009; 86(2): 273 - 281. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Mori, S. Tsuji, M. Inui, Y. Sakamoto, S. Endo, Y. Ito, S. Fujimura, T. Koga, A. Nakamura, H. Takayanagi, et al. Inhibitory Immunoglobulin-Like Receptors LILRB and PIR-B Negatively Regulate Osteoclast Development J. Immunol., October 1, 2008; 181(7): 4742 - 4751. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Morel and T. Bellon HLA Class I Molecules Regulate IFN-{gamma} Production Induced in NK Cells by Target Cells, Viral Products, or Immature Dendritic Cells through the Inhibitory Receptor ILT2/CD85j J. Immunol., August 15, 2008; 181(4): 2368 - 2381. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. van Stijn, A. T. Rowshani, S. L. Yong, F. Baas, E. Roosnek, I. J. M. ten Berge, and R. A. W. van Lier Human Cytomegalovirus Infection Induces a Rapid and Sustained Change in the Expression of NK Cell Receptors on CD8+ T Cells J. Immunol., April 1, 2008; 180(7): 4550 - 4560. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Tedla, C.-W. Lee, L. Borges, C. L. Geczy, and J. P. Arm Differential expression of leukocyte immunoglobulin-like receptors on cord blood-derived human mast cell progenitors and mature mast cells J. Leukoc. Biol., February 1, 2008; 83(2): 334 - 343. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Maffei, F. Ghiotto, M. Occhino, M. Bono, A. De Santanna, L. Battini, G. L. Gusella, F. Fais, S. Bruno, and E. Ciccone Human Cytomegalovirus Regulates Surface Expression of the Viral Protein UL18 by Means of Two Motifs Present in the Cytoplasmic Tail J. Immunol., January 15, 2008; 180(2): 969 - 979. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. S. Wagner, L. Walther-Jallow, E. Buentke, H.-G. Ljunggren, A. Achour, and B. J. Chambers Human cytomegalovirus-derived protein UL18 alters the phenotype and function of monocyte-derived dendritic cells J. Leukoc. Biol., January 1, 2008; 83(1): 56 - 63. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. S. Wagner, H.-G. Ljunggren, and A. Achour Immune Modulation by the Human Cytomegalovirus-Encoded Molecule UL18, a Mystery Yet to Be Solved J. Immunol., January 1, 2008; 180(1): 19 - 24. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Lichterfeld, D. G. Kavanagh, K. L. Williams, B. Moza, S. K. Mui, T. Miura, R. Sivamurthy, R. Allgaier, F. Pereyra, A. Trocha, et al. A viral CTL escape mutation leading to immunoglobulin-like transcript 4 mediated functional inhibition of myelomonocytic cells J. Exp. Med., November 26, 2007; 204(12): 2813 - 2824. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. S. Wagner, G. C. Riise, T. Bergstrom, K. Karre, E. Carbone, and L. Berg Increased Expression of Leukocyte Ig-Like Receptor-1 and Activating Role of UL18 in the Response to Cytomegalovirus Infection J. Immunol., March 15, 2007; 178(6): 3536 - 3543. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Bahri, F. Hirsch, A. Josse, N. Rouas-Freiss, N. Bidere, A. Vasquez, E. D. Carosella, B. Charpentier, and A. Durrbach Soluble HLA-G Inhibits Cell Cycle Progression in Human Alloreactive T Lymphocytes J. Immunol., February 1, 2006; 176(3): 1331 - 1339. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. E. Kirwan and D. N. Burshtyn Killer Cell Ig-Like Receptor-Dependent Signaling by Ig-Like Transcript 2 (ILT2/CD85j/LILRB1/LIR-1) J. Immunol., October 15, 2005; 175(8): 5006 - 5015. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Merlo, C. Tenca, F. Fais, L. Battini, E. Ciccone, C. E. Grossi, and D. Saverino Inhibitory Receptors CD85j, LAIR-1, and CD152 Down-Regulate Immunoglobulin and Cytokine Production by Human B Lymphocytes Clin. Vaccine Immunol., June 1, 2005; 12(6): 705 - 712. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Tenca, A. Merlo, E. Merck, E. E. M. Bates, D. Saverino, R. Simone, D. Zarcone, G. Trinchieri, C. E. Grossi, and E. Ciccone CD85j (Leukocyte Ig-Like Receptor-1/Ig-Like Transcript 2) Inhibits Human Osteoclast-Associated Receptor-Mediated Activation of Human Dendritic Cells J. Immunol., June 1, 2005; 174(11): 6757 - 6763. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Vales-Gomez, M. Shiroishi, K. Maenaka, and H. T. Reyburn Genetic Variability of the Major Histocompatibility Complex Class I Homologue Encoded by Human Cytomegalovirus Leads to Differential Binding to the Inhibitory Receptor ILT2 J. Virol., February 15, 2005; 79(4): 2251 - 2260. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Anfossi, J.-M. Doisne, M.-A. Peyrat, S. Ugolini, O. Bonnaud, D. Bossy, V. Pitard, P. Merville, J.-F. Moreau, J.-F. Delfraissy, et al. Coordinated Expression of Ig-Like Inhibitory MHC Class I Receptors and Acquisition of Cytotoxic Function in Human CD8+ T Cells J. Immunol., December 15, 2004; 173(12): 7223 - 7229. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. E. Sloane, N. Tedla, M. Awoniyi, D. W. MacGlashan Jr, L. Borges, K. F. Austen, and J. P. Arm Leukocyte immunoglobulin-like receptors: novel innate receptors for human basophil activation and inhibition Blood, November 1, 2004; 104(9): 2832 - 2839. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. LeMaoult, I. Krawice-Radanne, J. Dausset, and E. D. Carosella HLA-G1-expressing antigen-presenting cells induce immunosuppressive CD4+ T cells PNAS, May 4, 2004; 101(18): 7064 - 7069. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Saverino, F. Ghiotto, A. Merlo, S. Bruno, L. Battini, M. Occhino, M. Maffei, C. Tenca, S. Pileri, L. Baldi, et al. Specific Recognition of the Viral Protein UL18 by CD85j/LIR-1/ILT2 on CD8+ T Cells Mediates the Non-MHC-Restricted Lysis of Human Cytomegalovirus-Infected Cells J. Immunol., May 1, 2004; 172(9): 5629 - 5637. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Urosevic, J. Kamarashev, G. Burg, and R. Dummer Primary cutaneous CD8+ and CD56+ T-cell lymphomas express HLA-G and killer-cell inhibitory ligand, ILT2 Blood, March 1, 2004; 103(5): 1796 - 1798. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. C. Ibrahim, Y. Allory, F. Commo, B. Gattegno, P. Callard, and P. Paul Altered Pattern of Major Histocompatibility Complex Expression in Renal Carcinoma: Tumor-Specific Expression of the Nonclassical Human Leukocyte Antigen-G Molecule Is Restricted to Clear Cell Carcinoma While Up-Regulation of Other Major Histocompatibility Complex Antigens Is Primarily Distributed in All Subtypes of Renal Carcinoma Am. J. Pathol., February 1, 2003; 162(2): 501 - 508. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nikolova, P. Musette, M. Bagot, L. Boumsell, and A. Bensussan Engagement of ILT2/CD85j in Sezary syndrome cells inhibits their CD3/TCR signaling Blood, July 18, 2002; 100(3): 1019 - 1025. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Bellon, F. Kitzig, J. Sayos, and M. Lopez-Botet Mutational Analysis of Immunoreceptor Tyrosine-Based Inhibition Motifs of the Ig-Like Transcript 2 (CD85j) Leukocyte Receptor J. Immunol., April 1, 2002; 168(7): 3351 - 3359. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Tedla, K. Gibson, H. P. McNeil, D. Cosman, L. Borges, and J. P. Arm The Co-Expression of Activating and Inhibitory Leukocyte Immunoglobulin-Like Receptors in Rheumatoid Synovium Am. J. Pathol., February 1, 2002; 160(2): 425 - 431. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Urosevic, J. Willers, B. Mueller, W. Kempf, G. Burg, and R. Dummer HLA-G protein up-regulation in primary cutaneous lymphomas is associated with interleukin-10 expression in large cell T-cell lymphomas and indolent B-cell lymphomas Blood, January 15, 2002; 99(2): 609 - 617. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Saverino, A. Merlo, S. Bruno, V. Pistoia, C. E. Grossi, and E. Ciccone Dual Effect of CD85/Leukocyte Ig-Like Receptor-1/Ig-Like Transcript 2 and CD152 (CTLA-4) on Cytokine Production by Antigen-Stimulated Human T Cells J. Immunol., January 1, 2002; 168(1): 207 - 215. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Merlo, D. Saverino, C. Tenca, C. E. Grossi, S. Bruno, and E. Ciccone CD85/LIR-1/ILT2 and CD152 (Cytotoxic T Lymphocyte Antigen 4) Inhibitory Molecules Down-Regulate the Cytolytic Activity of Human CD4+ T-Cell Clones Specific for Mycobacterium tuberculosis Infect. Immun., October 1, 2001; 69(10): 6022 - 6029. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Aractingi, N. Briand, C. Le Danff, M. Viguier, H. Bachelez, L. Michel, L. Dubertret, and E. D. Carosella HLA-G and NK Receptor Are Expressed in Psoriatic Skin : A Possible Pathway for Regulating Infiltrating T Cells? Am. J. Pathol., July 1, 2001; 159(1): 71 - 77. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
