| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Laboratory of Immunology, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892
IL-4 is known to promote the differentiation of CD4+ T
cells into IL-4-secreting Th2 cells. However, the cellular source of
the early burst of IL-4 that drives Th2 responses in vivo has not been
conclusively identified. Mice deficient for the IL-4 receptor 
-chain
(IL-4R-/-) retain the capacity to secrete IL-4 and can
be used to identify those cell types that produce IL-4 without a
requirement for prior IL-4-mediated stimulation. To address whether
naive, conventional CD4+ T cells may act as initial
producers of IL-4 in Ag-specific responses, we crossed the BALB/c
IL-4R-/-mice to DO11.10/scid TCR
transgenic mice. Lymph node cells from wild-type and
IL-4R-/- DO11.10/scid mice secreted
50 pg of IL-4 per106 cells within 48 h after
peptide stimulation. This small amount of IL-4 was sufficient to cause
the differentiation of wild-type CD4+ T cells into Th2
cells, particularly if IFN-
and IL-12 were neutralized during the
priming cultures. CD4+ cells from the
IL-4R-/- mice gave rise to a minor proportion (2%)
of IL-4-producing cells upon stimulation in the presence of
anti-IFN- and anti-IL-12. These data show that conventional,
naive CD4+ T cells may be considered as initial sources of
IL-4 and, in the absence of IFN- and IL-12, this IL-4 can induce Th2
polarization.
This article has been cited by other articles:
|
|
 |
|
  K. Honma, D. Kimura, N. Tominaga, M. Miyakoda, T. Matsuyama, and K. Yui Interferon regulatory factor 4 differentially regulates the production of Th2 cytokines in naive vs. effector/memory CD4+ T cells PNAS, October 14, 2008; 105(41): 15890 - 15895. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Xin, K. Ohmori, J. Nishida, Y. Zhu, and H. Huang The initial response of CD4+ IL-4-producing cells Int. Immunol., March 1, 2007; 19(3): 305 - 310. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Sun and E. J. Pearce Suppression of Early IL-4 Production Underlies the Failure of CD4 T Cells Activated by TLR-Stimulated Dendritic Cells to Differentiate into Th2 Cells J. Immunol., February 1, 2007; 178(3): 1635 - 1644. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Balic, Y. M. Harcus, M. D. Taylor, F. Brombacher, and R. M. Maizels IL-4R signaling is required to induce IL-10 for the establishment of Th2 dominance Int. Immunol., October 1, 2006; 18(10): 1421 - 1431. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Voehringer, T. A. Reese, X. Huang, K. Shinkai, and R. M. Locksley Type 2 immunity is controlled by IL-4/IL-13 expression in hematopoietic non-eosinophil cells of the innate immune system J. Exp. Med., June 12, 2006; 203(6): 1435 - 1446. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. So, J. Song, K. Sugie, A. Altman, and M. Croft Signals from OX40 regulate nuclear factor of activated T cells c1 and T cell helper 2 lineage commitment PNAS, March 7, 2006; 103(10): 3740 - 3745. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Foley, U. Jung, A. Miera, T. Borenstein, J. Mariotti, M. Eckhaus, B. E. Bierer, and D. H. Fowler Ex Vivo Rapamycin Generates Donor Th2 Cells That Potently Inhibit Graft-versus-Host Disease and Graft-versus-Tumor Effects via an IL-4-Dependent Mechanism J. Immunol., November 1, 2005; 175(9): 5732 - 5743. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Liu, Q. Liu, H. Hamed, R. M. Anthony, A. Foster, F. D. Finkelman, J. F. Urban Jr, and W. C. Gause IL-2 and Autocrine IL-4 Drive the In Vivo Development of Antigen-Specific Th2 T Cells Elicited by Nematode Parasites J. Immunol., February 15, 2005; 174(4): 2242 - 2249. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R. Patel, M. H. Kaplan, and C.-H. Chang Altered Th1 Cell Differentiation Programming by CIITA Deficiency J. Immunol., November 1, 2004; 173(9): 5501 - 5508. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Rivas-Carvalho, M. A. Meraz-Rios, L. Santos-Argumedo, S. Bajana, G. Soldevila, M. E. Moreno-Garcia, and C. Sanchez-Torres CD16+ human monocyte-derived dendritic cells matured with different and unrelated stimuli promote similar allogeneic Th2 responses: regulation by pro- and anti-inflammatory cytokines Int. Immunol., September 1, 2004; 16(9): 1251 - 1263. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Jankovic, M. C. Kullberg, P. Caspar, and A. Sher Parasite-Induced Th2 Polarization Is Associated with Down-Regulated Dendritic Cell Responsiveness to Th1 Stimuli and a Transient Delay in T Lymphocyte Cycling J. Immunol., August 15, 2004; 173(4): 2419 - 2427. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Kelly-Welch, M. E. F. Melo, E. Smith, A. Q. Ford, C. Haudenschild, N. Noben-Trauth, and A. D. Keegan Complex Role of the IL-4 Receptor {alpha} in a Murine Model of Airway Inflammation: Expression of the IL-4 Receptor {alpha} on Nonlymphoid Cells of Bone Marrow Origin Contributes to Severity of Inflammation J. Immunol., April 1, 2004; 172(7): 4545 - 4555. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. Manfras, S. Reuter, T. Wendland, B. O. Boehm, and P. Kern Impeded Th1 CD4 memory T cell generation in chronic-persisting liver infection with Echinococcus multilocularis Int. Immunol., January 1, 2004; 16(1): 43 - 50. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Maszyna, H. Hoff, D. Kunkel, A. Radbruch, and M. C. Brunner-Weinzierl Diversity of Clonal T Cell Proliferation Is Mediated by Differential Expression of CD152 (CTLA-4) on the Cell Surface of Activated Individual T Lymphocytes J. Immunol., October 1, 2003; 171(7): 3459 - 3466. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Noben-Trauth, R. Lira, H. Nagase, W. E. Paul, and D. L. Sacks The Relative Contribution of IL-4 Receptor Signaling and IL-10 to Susceptibility to Leishmania major J. Immunol., May 15, 2003; 170(10): 5152 - 5158. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.-J. Linton, B. Bautista, E. Biederman, E. S. Bradley, J. Harbertson, R. M. Kondrack, R. C. Padrick, and L. M. Bradley Costimulation via OX40L Expressed by B Cells Is Sufficient to Determine the Extent of Primary CD4 Cell Expansion and Th2 Cytokine Secretion In Vivo J. Exp. Med., April 7, 2003; 197(7): 875 - 883. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Metwali, A. Blum, D. E. Elliott, and J. V. Weinstock Interleukin-4 Receptor {alpha} Chain and STAT6 Signaling Inhibit Gamma Interferon but Not Th2 Cytokine Expression within Schistosome Granulomas Infect. Immun., October 1, 2002; 70(10): 5651 - 5658. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Venuprasad, P. P. Banerjee, S. Chattopadhyay, S. Sharma, S. Pal, P. B. Parab, D. Mitra, and B. Saha Human Neutrophil-Expressed CD28 Interacts with Macrophage B7 to Induce Phosphatidylinositol 3-Kinase-Dependent IFN-{gamma} Secretion and Restriction of Leishmania Growth J. Immunol., July 15, 2002; 169(2): 920 - 928. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Brogdon, D. Leitenberg, and K. Bottomly The Potency of TCR Signaling Differentially Regulates NFATc/p Activity and Early IL-4 Transcription in Naive CD4+ T Cells J. Immunol., April 15, 2002; 168(8): 3825 - 3832. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Y. Karulin, M. D. Hesse, H. C. Yip, and P. V. Lehmann Indirect IL-4 Pathway in Type 1 Immunity J. Immunol., January 15, 2002; 168(2): 545 - 553. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Henderson, M. M. Kamdar, and A. Bamezai Ly-6A.2 Expression Regulates Antigen-Specific CD4+ T Cell Proliferation and Cytokine Production J. Immunol., January 1, 2002; 168(1): 118 - 126. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Foucras, A. Gallard, C. Coureau, J.-M. Kanellopoulos, and J.-C. Guery Chronic Soluble Antigen Sensitization Primes a Unique Memory/Effector T Cell Repertoire Associated with Th2 Phenotype Acquisition In Vivo J. Immunol., January 1, 2002; 168(1): 179 - 187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. B. Campbell, T. Komata, and A. Kelso CD4 Ligation Promotes the IL-4-Independent Development of IL-4-Producing Clones from Naive CD4+ T Cells J. Immunol., November 15, 2001; 167(10): 5610 - 5619. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Maldonado-Lopez, C. Maliszewski, J. Urbain, and M. Moser Cytokines Regulate the Capacity of CD8{alpha}+ and CD8{alpha}- Dendritic Cells to Prime Th1/Th2 Cells In Vivo J. Immunol., October 15, 2001; 167(8): 4345 - 4350. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
