| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
B in Cytokine Production Induced from Human Airway Epithelial Cells by Rhinovirus Infection1
Divisions of
*
Clinical Immunology and
Pulmonary and Critical Care Medicine, Department of Medicine, and
Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, MD 21224
Infection of human epithelial cells with human rhinovirus (HRV)-16
induces rapid production of several proinflammatory cytokines,
including IL-8, IL-6, and GM-CSF. We evaluated the role of NF-
B in
HRV-16-induced IL-8 and IL-6 production by EMSA using oligonucleotides
corresponding to the binding sites for NF-B in the IL-6 and IL-8
gene promoters. Consistent with the rapid induction of mRNA for IL-8
and IL-6, maximal NF-B binding to both oligonucleotides was detected
at 30 min after infection. NF-B complexes contained p65 and p50, but
not c-Rel. The IL-8 oligonucleotide bound recombinant p50 with only
about one-tenth the efficiency of the IL-6 oligonucleotide, even though
epithelial cells produced more IL-8 protein than IL-6. Neither the
potent glucocorticoid, budesonide (10-7 M), nor a NO donor
inhibited NF-B binding to either cytokine promoter or induction of
mRNA for either IL-8 or IL-6. Sulfasalazine and calpain inhibitor I,
inhibitors of NF-B activation, blocked HRV-16-induced formation of
NF-B complexes with oligonucleotides from both cytokines, but did
not inhibit mRNA induction for either cytokine. By contrast,
sulfasalazine clearly inhibited HRV-16 induction of mRNA for GM-CSF in
the same cells. Thus, HRV-16 induces epithelial expression of IL-8 and
IL-6 by an NF-B-independent pathway, whereas induction of GM-CSF is
at least partially dependent upon NF-B
activation.
This article has been cited by other articles:
|
|
 |
|
  M. R. Edwards, J. Haas, R. A. Panettieri Jr., M. Johnson, and S. L. Johnston Corticosteroids and beta2 Agonists Differentially Regulate Rhinovirus-induced Interleukin-6 via Distinct Cis-acting Elements J. Biol. Chem., May 25, 2007; 282(21): 15366 - 15375. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. S. Sajjan, Y. Jia, D. C. Newcomb, J. K. Bentley, N. W. Lukacs, J. J. LiPuma, and M. B. Hershenson H. influenzae potentiates airway epithelial cell responses to rhinovirus by increasing ICAM-1 and TLR3 expression FASEB J, October 1, 2006; 20(12): 2121 - 2123. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Laza-Stanca, L. A. Stanciu, S. D. Message, M. R. Edwards, J. E. Gern, and S. L. Johnston Rhinovirus Replication in Human Macrophages Induces NF-{kappa}B-Dependent Tumor Necrosis Factor Alpha Production. J. Virol., August 1, 2006; 80(16): 8248 - 8258. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Peng, S. Kotla, R. E. Bumgarner, and K. E. Gustin Human rhinovirus attenuates the type I interferon response by disrupting activation of interferon regulatory factor 3. J. Virol., May 1, 2006; 80(10): 5021 - 5031. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Edwards, M. W. Johnson, and S. L. Johnston Combination Therapy: Synergistic Suppression of Virus-Induced Chemokines in Airway Epithelial Cells Am. J. Respir. Cell Mol. Biol., May 1, 2006; 34(5): 616 - 624. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Chen, E. Hamati, P.-K. Lee, W.-M. Lee, S. Wachi, D. Schnurr, S. Yagi, G. Dolganov, H. Boushey, P. Avila, et al. Rhinovirus Induces Airway Epithelial Gene Expression through Double-Stranded RNA and IFN-Dependent Pathways Am. J. Respir. Cell Mol. Biol., February 1, 2006; 34(2): 192 - 203. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. Newcomb, U. Sajjan, S. Nanua, Y. Jia, A. M. Goldsmith, J. K. Bentley, and M. B. Hershenson Phosphatidylinositol 3-Kinase Is Required for Rhinovirus-induced Airway Epithelial Cell Interleukin-8 Expression J. Biol. Chem., November 4, 2005; 280(44): 36952 - 36961. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Hewson, A. Jardine, M. R. Edwards, V. Laza-Stanca, and S. L. Johnston Toll-Like Receptor 3 Is Induced by and Mediates Antiviral Activity against Rhinovirus Infection of Human Bronchial Epithelial Cells J. Virol., October 1, 2005; 79(19): 12273 - 12279. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Johnston Overview of Virus-induced Airway Disease Proceedings of the ATS, August 1, 2005; 2(2): 150 - 156. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. L. Spurrell, S. Wiehler, R. S. Zaheer, S. P. Sanders, and D. Proud Human airway epithelial cells produce IP-10 (CXCL10) in vitro and in vivo upon rhinovirus infection Am J Physiol Lung Cell Mol Physiol, July 1, 2005; 289(1): L85 - L95. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Hall, M. E. Bates, L. Guar, M. Cronan, N. Korpi, and P. J. Bertics The Role of p38 MAPK in Rhinovirus-Induced Monocyte Chemoattractant Protein-1 Production by Monocytic-Lineage Cells J. Immunol., June 15, 2005; 174(12): 8056 - 8063. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. G. Cosio, B. Mann, K. Ito, E. Jazrawi, P. J. Barnes, K. F. Chung, and I. M. Adcock Histone Acetylase and Deacetylase Activity in Alveolar Macrophages and Blood Mononocytes in Asthma Am. J. Respir. Crit. Care Med., July 15, 2004; 170(2): 141 - 147. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Proud, S. P. Sanders, and S. Wiehler Human Rhinovirus Infection Induces Airway Epithelial Cell Production of Human {beta}-Defensin 2 Both In Vitro and In Vivo J. Immunol., April 1, 2004; 172(7): 4637 - 4645. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I S Patel Viral regulation of inflammatory cytokines in epithelial cells: an alternative signalling pathway Thorax, December 1, 2003; 58(12): 1019 - 1019. [Full Text]
|
|
|
|
|
|
T. R. Meusel and F. Imani Viral Induction of Inflammatory Cytokines in Human Epithelial Cells Follows a p38 Mitogen-Activated Protein Kinase-Dependent but NF-{kappa}B-Independent Pathway J. Immunol., October 1, 2003; 171(7): 3768 - 3774. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zhou, A. Tan, S. Iasvovskaia, J. Li, A. Lin, and M. B. Hershenson Ras and Mitogen-Activated Protein Kinase Kinase Kinase-1 Coregulate Activator Protein-1- and Nuclear Factor-{kappa}B-Mediated Gene Expression in Airway Epithelial Cells Am. J. Respir. Cell Mol. Biol., June 1, 2003; 28(6): 762 - 769. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P.G. Gibson, P.A.B. Wark, J.L. Simpson, C. Meldrum, S. Meldrum, N. Saltos, and M. Boyle Induced sputum IL-8 gene expression, neutrophil influx and MMP-9 in allergic bronchopulmonary aspergillosis Eur. Respir. J., April 1, 2003; 21(4): 582 - 588. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. V. Culpitt, D. F. Rogers, P. Shah, C. De Matos, R. E. K. Russell, L. E. Donnelly, and P. J. Barnes Impaired Inhibition by Dexamethasone of Cytokine Release by Alveolar Macrophages from Patients with Chronic Obstructive Pulmonary Disease Am. J. Respir. Crit. Care Med., January 1, 2003; 167(1): 24 - 31. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Sarady, S. L. Otterbein, F. Liu, L. E. Otterbein, and A. M. K. Choi Carbon Monoxide Modulates Endotoxin-Induced Production of Granulocyte Macrophage Colony-Stimulating Factor in Macrophages Am. J. Respir. Cell Mol. Biol., December 1, 2002; 27(6): 739 - 745. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Sanders, J. Kim, K. Ryan Connolly, J. D. Porter, E. S. Siekierski, and D. Proud Nitric Oxide Inhibits Rhinovirus-Induced Granulocyte Macrophage Colony-Stimulating Factor Production in Bronchial Epithelial Cells Am. J. Respir. Cell Mol. Biol., March 1, 2001; 24(3): 317 - 325. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
