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Independent Selection by I-A^k Molecules of Two Epitopes Found in Tandem in an Extended Polypeptide Antigen¹

Raffi Gugasyan^2,3,*, Carlos Velazquez^2,*, Ilan Vidavsky^2,, Brian M. Deck^*, Koen van der Drift, Michael L. Gross^4,*, and Emil R. Unanue^*

Departments of ^* Pathology and Immunology and Chemistry, Washington University School of Medicine, St. Louis, MO 63110

The protein hen egg white lysozyme (HEL) contains two segments, in tandem, from which two families of peptides are selected by the class II molecule I-A^k, during processing. These encompass peptides primarily from residues 31–47 and 48–63. Mutant HEL proteins were created with changes in residues 52 and 55, resulting in a lack of binding and selection of the 48–63 peptides to I-A^k molecules. Such mutant HEL proteins donated the same amount of 31–47 peptide as did the unmodified protein. Other mutant HEL molecules containing proline residues at residue 46, 47, or 48 resulted in extensions of the selected 31–47 or 48–62 families to their overlapping regions (in the carboxyl or amino termini, respectively). However, the amount of each family of peptide selected was not changed. We conclude that the presence or absence of the major peptide from HEL does not influence the selection of other epitopes, and that these two families are selected independently of each other.

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