| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Chemoattractant for Human Neutrophils and Dendritic Cells1
Department of Immunology and Shanghai Brilliance Biotechnology Institute, Second Military Medical University, Shanghai, People’s Republic of China
Chemokines play important roles in leukocyte trafficking as well as
function regulation. In this study, we described the identification and
characterization of a novel CXC chemokine from a human dendritic cell
(DC) cDNA library, the full-length cDNA of which contains an open
reading frame encoding 111 aa with a putative signal peptide of 34 aa.
This CXC chemokine shares greatest homology with macrophage
inflammatory protein (MIP)-2
ß, hence is designated as MIP-2
.
Mouse MIP-2 was identified by electrocloning and is highly
homologous to human MIP-2. Northern blotting revealed that MIP-2
was constitutively and widely expressed in most normal tissues with the
greatest expression in kidney, but undetectable in most tumor cell
lines except THP-1 cells. In situ hybridization analysis demonstrated
that MIP-2 was mainly expressed by the epithelium of tubules in the
kidney and hepatocytes in the liver. Although no detectable expression
was observed in freshly isolated or PMA-treated monocytes, RT-PCR
analysis revealed MIP-2 expression by monocyte-derived DC.
Recombinant MIP-2 from 293 cells is about 9.5 kDa in size and
specifically detectable by its polyclonal Ab developed by the
immunization with its 6His-tagged fusion protein. The eukaryotically
expressed MIP-2 is a potent chemoattractant for neutrophils, and
weaker for DC, but inactive to monocytes, NK cells, and T and B
lymphocytes. Receptor binding assays showed that MIP-2 does not bind
to CXCR2. This implies that DC might contribute to the innate immunity
through the production of neutrophil-attracting chemokines and extends
the knowledge about the regulation of DC
migration.
This article has been cited by other articles:
|
|
 |
|
  G. Helguera, M. Eghbali, D. Sforza, T. Y. Minosyan, L. Toro, and E. Stefani Changes in global gene expression in rat myometrium in transition from late pregnancy to parturition Physiol Genomics, January 1, 2009; 36(2): 89 - 97. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Nara, Y. Nakayama, S. Okamoto, H. Tamura, M. Kiyono, M. Muraoka, K. Tanaka, C. Taya, H. Shitara, R. Ishii, et al. Disruption of CXC Motif Chemokine Ligand-14 in Mice Ameliorates Obesity-induced Insulin Resistance J. Biol. Chem., October 19, 2007; 282(42): 30794 - 30803. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Meuter, P. Schaerli, R. S. Roos, O. Brandau, M. R. Bosl, U. H. von Andrian, and B. Moser Murine CXCL14 Is Dispensable for Dendritic Cell Function and Localization within Peripheral Tissues Mol. Cell. Biol., February 1, 2007; 27(3): 983 - 992. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. H. Durand, V. Flacher, A. Romeas, F. Carrouel, E. Colomb, C. Vincent, H. Magloire, M.-L. Couble, F. Bleicher, M.-J. Staquet, et al. Lipoteichoic Acid Increases TLR and Functional Chemokine Expression while Reducing Dentin Formation in In Vitro Differentiated Human Odontoblasts. J. Immunol., March 1, 2006; 176(5): 2880 - 2887. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. C. Wesselkamper, S. A. McDowell, M. Medvedovic, T. P. Dalton, H. S. Deshmukh, M. A. Sartor, L. M. Case, L. N. Henning, M. T. Borchers, C. R. Tomlinson, et al. The Role of Metallothionein in the Pathogenesis of Acute Lung Injury Am. J. Respir. Cell Mol. Biol., January 1, 2006; 34(1): 73 - 82. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. M. Schmidt-Ott, J. Yang, X. Chen, H. Wang, N. Paragas, K. Mori, J.-Y. Li, B. Lu, F. Costantini, M. Schiffer, et al. Novel Regulators of Kidney Development from the Tips of the Ureteric Bud J. Am. Soc. Nephrol., July 1, 2005; 16(7): 1993 - 2002. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. V. Shurin, R. Ferris, I. L. Tourkova, L. Perez, A. Lokshin, L. Balkir, B. Collins, G. S. Chatta, and M. R. Shurin Loss of New Chemokine CXCL14 in Tumor Tissue Is Associated with Low Infiltration by Dendritic Cells (DC), while Restoration of Human CXCL14 Expression in Tumor Cells Causes Attraction of DC Both In Vitro and In Vivo J. Immunol., May 1, 2005; 174(9): 5490 - 5498. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. D. Shellenberger, M. Wang, M. Gujrati, A. Jayakumar, R. M. Strieter, M. D. Burdick, C. G. Ioannides, C. L. Efferson, A. K. El-Naggar, D. Roberts, et al. BRAK/CXCL14 Is a Potent Inhibitor of Angiogenesis and a Chemotactic Factor for Immature Dendritic Cells Cancer Res., November 15, 2004; 64(22): 8262 - 8270. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Han, M. Zhang, N. Li, T. Chen, Y. Zhang, T. Wan, and X. Cao KLRL1, a novel killer cell lectinlike receptor, inhibits natural killer cell cytotoxicity Blood, November 1, 2004; 104(9): 2858 - 2866. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Wan, X. Zhou, G. Chen, H. An, T. Chen, W. Zhang, S. Liu, Y. Jiang, F. Yang, Y. Wu, et al. Novel heat shock protein Hsp70L1 activates dendritic cells and acts as a Th1 polarizing adjuvant Blood, March 1, 2004; 103(5): 1747 - 1754. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Chen, J. Guo, M. Yang, C. Han, M. Zhang, W. Chen, Q. Liu, J. Wang, and X. Cao Cyclosporin A impairs dendritic cell migration by regulating chemokine receptor expression and inhibiting cyclooxygenase-2 expression Blood, January 15, 2004; 103(2): 413 - 421. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Girotti and H. H. Zingg Gene Expression Profiling of Rat Uterus at Different Stages of Parturition Endocrinology, June 1, 2003; 144(6): 2254 - 2265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Li, W. Zhang, T. Wan, J. Zhang, T. Chen, Y. Yu, J. Wang, and X. Cao Cloning and Characterization of Siglec-10, a Novel Sialic Acid Binding Member of the Ig Superfamily, from Human Dendritic Cells J. Biol. Chem., July 20, 2001; 276(30): 28106 - 28112. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
