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The IgA/IgM Receptor Expressed on a Murine B Cell Lymphoma Is Poly-Ig Receptor¹

Julia M. Phillips-Quagliata^2,*, Samir Patel^*, Jing-Kang Han^*, Sergei Arakelov^*, T. Dharma Rao^*, Marc J. Shulman, Shafie Fazel, Ronald B. Corley, Margaret Everett^§, Michel H. Klein^§, Brian J. Underdown^¶ and Blaise Corthésy^||

^* Department of Pathology, New York University School of Medicine and Kaplan Cancer Center, New York, NY 10016; Department of Immunology and Medical Genetics, University of Toronto, Toronto, Ontario, Canada; Boston University Medical Center, Boston, MA 02118; ^§ Department of Immunology, University of Toronto, Toronto, Ontario, Canada; ^¶ Department of Pathology, McMaster University, Hamilton, Ontario, Canada; and ^|| Division of Immunology and Allergy, University Hospital, Lausanne, Switzerland

T560, a mouse B lymphoma that originated in gut-associated lymphoid tissue, expresses receptors that bind dimeric IgA and IgM in a mutually inhibitory manner but have little affinity for monomeric IgA. Evidence presented in this paper indicates that the receptor is poly-Ig receptor (pIgR) known in humans and domestic cattle to bind both IgA and IgM. The evidence includes the demonstration that binding of IgM is J chain dependent, and that pIg-precipitated receptor has an appropriate M_r of 116–120 kDa and can be detected on immunoblots with specific rabbit anti-mouse pIgR. Overlapping RT-PCR performed using template mRNA from T560 cells and oligonucleotide primer pairs designed from the published sequence of mouse liver pIgR indicate that T560 cells express mRNA virtually identical with that of the epithelial cell pIgR throughout its external, transmembrane, and intracytoplasmic coding regions. Studies using mutant IgAs suggest that the C2 domain of dimeric IgA is not involved in high-affinity binding to the T560 pIgR. Inasmuch as this mouse B cell pIgR binds IgM better than IgA, it is similar to human pIgR and differs from rat, mouse, and rabbit epithelial cell pIgRs that bind IgA but not IgM. Possible explanations for this difference are discussed. All clones of T560 contain some cells that spontaneously secrete both IgG2a and IgA, but all of the IgA recoverable from the medium and from cell lysates is monomeric; it cannot be converted to secretory IgA by T560 cells.

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