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ß Repertoires and Differential Distribution in Lymphoid Tissues1
*
Unité de Biologie Moléculaire du Gène, Institut National de la Santé et de la Recherche Médicale Unité 277, and Institut Pasteur, Paris, France; and the
Unité du Développement des Lymphocytes, Institut Pasteur, Paris, France
NKT cells are a subset of T lymphocytes that is mainly restricted
by the nonclassical MHC class I molecule, CD1d, and that includes
several subpopulations, in particular CD4+ and
CD4-CD8- (DN) cells. In the mouse,
differential distribution of these subpopulations as well as
heterogeneity in the expression of various markers as a function of
tissue localization have been reported. We have thus undertaken a
detailed study of the DN NKT cell subpopulation. With a highly
sensitive semiquantitative RT-PCR technique, its TCR repertoire was
characterized in various tissues. We found that mouse DN NKT cells are
a variable mixture of two subgroups, one bearing the invariant V
14
chain paired to rearranged Vß2, Vß7, Vß8.1, Vß8.2, or Vß8.3
ß-chains and the other exhibiting unskewed - and ß-chains. The
proportion of these subgroups varies from about 100:0 in thymus, 80:20
in liver, and 50:50 in spleen to 20:80% in bone marrow, respectively.
Finally, further heterogeneity in the tissue-derived DN NKT cells was
discovered by sequencing extensively Vß8.2-Jß2.5 rearrangements in
individual mice. Despite a few recurrences in TCR sequences, we found
that each population exhibits its own and broad TCRß
diversity.
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