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*
Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus, Denmark;
Department of Microbiology and Immunology, University of Leicester, Leicester, United Kingdom;
Medical Research Council Immunochemistry Unit, University of Oxford, Oxford, United Kingdom; and
§
Molecular Sciences Division, Nuffield Department of Clinical Medicine, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom
Mannan-binding lectin (MBL) plays a pivotal role in innate immunity by activating complement after binding carbohydrate moieties on pathogenic bacteria and viruses. Structural similarities shared by MBL and C1 complexes and by the MBL- and C1q-associated serine proteases, MBL-associated serine protease (MASP)-1 and MASP-2, and C1r and C1s, respectively, have led to the expectation that the pathways of complement activation by MBL and C1 complexes are likely to be very similar. We have expressed rMASP-2 and show that, whereas C1 complex autoactivation proceeds via a two-step mechanism requiring proteolytic activation of both C1r and C1s, reconstitution with MASP-2 alone is sufficient for complement activation by MBL. The results suggest that the catalytic activities of MASP-2 split between the two proteases of the C1 complex during the course of vertebrate complement evolution.
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N. J. Lynch, S.-u.-H. Khan, C. M. Stover, S. M. Sandrini, D. Marston, J. S. Presanis, and W. J. Schwaeble Composition of the Lectin Pathway of Complement in Gallus gallus: Absence of Mannan-Binding Lectin-Associated Serine Protease-1 in Birds J. Immunol., April 15, 2005; 174(8): 4998 - 5006. [Abstract] [Full Text] [PDF]
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L. A. Gregory, N. M. Thielens, M. Matsushita, R. Sorensen, G. J. Arlaud, J. C. Fontecilla-Camps, and C. Gaboriaud The X-ray Structure of Human Mannan-binding Lectin-associated Protein 19 (MAp19) and Its Interaction Site with Mannan-binding Lectin and L-ficolin J. Biol. Chem., July 9, 2004; 279(28): 29391 - 29397. [Abstract] [Full Text] [PDF]
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N. J. Lynch, S. Roscher, T. Hartung, S. Morath, M. Matsushita, D. N. Maennel, M. Kuraya, T. Fujita, and W. J. Schwaeble L-Ficolin Specifically Binds to Lipoteichoic Acid, a Cell Wall Constituent of Gram-Positive Bacteria, and Activates the Lectin Pathway of Complement J. Immunol., January 15, 2004; 172(2): 1198 - 1202. [Abstract] [Full Text] [PDF]
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Y. Endo, M. Nonaka, H. Saiga, Y. Kakinuma, A. Matsushita, M. Takahashi, M. Matsushita, and T. Fujita Origin of Mannose-Binding Lectin-Associated Serine Protease (MASP)-1 and MASP-3 Involved in the Lectin Complement Pathway Traced Back to the Invertebrate, Amphioxus J. Immunol., May 1, 2003; 170(9): 4701 - 4707. [Abstract] [Full Text] [PDF]
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G. Ambrus, P. Gal, M. Kojima, K. Szilagyi, J. Balczer, J. Antal, L. Graf, A. Laich, B. E. Moffatt, W. Schwaeble, et al. Natural Substrates and Inhibitors of Mannan-Binding Lectin-Associated Serine Protease-1 and -2: A Study on Recombinant Catalytic Fragments J. Immunol., February 1, 2003; 170(3): 1374 - 1382. [Abstract] [Full Text] [PDF]
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K. Uemura, M. Saka, T. Nakagawa, N. Kawasaki, S. Thiel, J. C. Jensenius, and T. Kawasaki L-MBP Is Expressed in Epithelial Cells of Mouse Small Intestine J. Immunol., December 15, 2002; 169(12): 6945 - 6950. [Abstract] [Full Text] [PDF]
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M. Collin, M. D. Svensson, A. G. Sjoholm, J. C. Jensenius, U. Sjobring, and A. Olsen EndoS and SpeB from Streptococcus pyogenes Inhibit Immunoglobulin-Mediated Opsonophagocytosis Infect. Immun., December 1, 2002; 70(12): 6646 - 6651. [Abstract] [Full Text] [PDF]
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S. Cseh, L. Vera, M. Matsushita, T. Fujita, G. J. Arlaud, and N. M. Thielens Characterization of the Interaction Between L-Ficolin/P35 and Mannan-Binding Lectin-Associated Serine Proteases-1 and -2 J. Immunol., November 15, 2002; 169(10): 5735 - 5743. [Abstract] [Full Text] [PDF]
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Y. Endo, M. Takahashi, M. Kuraya, M. Matsushita, C. M. Stover, W. J. Schwaeble, and T. Fujita Functional characterization of human mannose-binding lectin-associated serine protease (MASP)-1/3 and MASP-2 promoters, and comparison with the C1s promoter Int. Immunol., October 1, 2002; 14(10): 1193 - 1201. [Abstract] [Full Text] [PDF]
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S. Gulati, K. Sastry, J. C. Jensenius, P. A. Rice, and S. Ram Regulation of the Mannan-Binding Lectin Pathway of Complement on Neisseria gonorrhoeae by C1-Inhibitor and {alpha}2-Macroglobulin J. Immunol., April 15, 2002; 168(8): 4078 - 4086. [Abstract] [Full Text] [PDF]
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M. Matsushita, M. Kuraya, N. Hamasaki, M. Tsujimura, H. Shiraki, and T. Fujita Activation of the Lectin Complement Pathway by H-Ficolin (Hakata Antigen) J. Immunol., April 1, 2002; 168(7): 3502 - 3506. [Abstract] [Full Text] [PDF]
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I. Celik, C. Stover, M. Botto, S. Thiel, S. Tzima, D. Kunkel, M. Walport, W. Lorenz, and W. Schwaeble Role of the Classical Pathway of Complement Activation in Experimentally Induced Polymicrobial Peritonitis Infect. Immun., December 1, 2001; 69(12): 7304 - 7309. [Abstract] [Full Text] [PDF]
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A. Roos, L. H. Bouwman, D. J. van Gijlswijk-Janssen, M. C. Faber-Krol, G. L. Stahl, and M. R. Daha Human IgA Activates the Complement System Via the Mannan-Binding Lectin Pathway J. Immunol., September 1, 2001; 167(5): 2861 - 2868. [Abstract] [Full Text] [PDF]
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N. M. Thielens, S. Cseh, S. Thiel, T. Vorup-Jensen, V. Rossi, J. C. Jensenius, and G. J. Arlaud Interaction Properties of Human Mannan-Binding Lectin (MBL)-Associated Serine Proteases-1 and -2, MBL-Associated Protein 19, and MBL J. Immunol., April 15, 2001; 166(8): 5068 - 5077. [Abstract] [Full Text] [PDF]
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V. Rossi, S. Cseh, I. Bally, N. M. Thielens, J. C. Jensenius, and G. J. Arlaud Substrate Specificities of Recombinant Mannan-binding Lectin-associated Serine Proteases-1 and -2 J. Biol. Chem., October 26, 2001; 276(44): 40880 - 40887. [Abstract] [Full Text] [PDF]
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