| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Unité de Biologie Moléculaire du Gène, Institut National de la Santé et de la Recherche Médicale, Unité 277, Institut Pasteur, Paris, France;
Institut National de la Santé et de la Recherche Médicale, Unité 463, Institut de Biologie, Nantes, France; and
Immunotech SA, Marseilles, France
In an attempt to provide a global picture of the TCR repertoire
diversity of a chronic T cell response against a common Ag, we
performed an extensive TCR analysis of cells reactive against a
dominant HLA-A2-restricted EBV epitope (hereafter referred to as
GLC/A2), obtained after sorting PBL or synovial fluid lymphocytes from
EBV-seropositive individuals using MHC/peptide multimers. Although TCR
ß-chain diversity of GLC/A2+ T cells was extensive and
varied greatly from one donor to another, we identified in most cell
lines several recurrent Vß subsets (Vß2, Vß4, and Vß16
positive) with highly conserved TCRß complementarity-determining
region 3 (CDR3) length and junctional motifs, which represented from 11
to 98% (mean, 50%) of GLC/A2-reactive cells. While TCR ß-chains
expressed by these subsets showed limited CDR1, CDR2, and CDR3 homology
among themselves, their TCR 
-chains comprised the same TCRAV region,
thus suggesting hierarchical contribution of TCR -chain vs TCR
ß-chain CDR to recognition of this particular MHC/peptide complex.
The common occurrence of T cell clonotypes with public TCR features
within GLC/A2-specific T cells allowed their direct detection within
unsorted PBL using ad hoc clonotypic primers. These results, which
suggest an unexpectedly high contribution of public clonotypes to the
TCR repertoire against a dominant epitope, have several implications
for the follow-up and modulation of T cell-mediated
immunity.
This article has been cited by other articles:
|
|
 |
|
  V. Venturi, H. Y. Chin, D. A. Price, D. C. Douek, and M. P. Davenport The Role of Production Frequency in the Sharing of Simian Immunodeficiency Virus-Specific CD8+ TCRs between Macaques J. Immunol., August 15, 2008; 181(4): 2597 - 2609. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Venturi, K. Kedzierska, D. A. Price, P. C. Doherty, D. C. Douek, S. J. Turner, and M. P. Davenport Sharing of T cell receptors in antigen-specific responses is driven by convergent recombination PNAS, December 5, 2006; 103(49): 18691 - 18696. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Speiser, P. Baumgaertner, C. Barbey, V. Rubio-Godoy, A. Moulin, P. Corthesy, E. Devevre, P.-Y. Dietrich, D. Rimoldi, D. Lienard, et al. A Novel Approach to Characterize Clonality and Differentiation of Human Melanoma-Specific T Cell Responses: Spontaneous Priming and Efficient Boosting by Vaccination J. Immunol., July 15, 2006; 177(2): 1338 - 1348. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Schaft, B. Lankiewicz, J. Drexhage, C. Berrevoets, D. J. Moss, V. Levitsky, M. Bonneville, S. P. Lee, A. J. McMichael, J.-W. Gratama, et al. T cell re-targeting to EBV antigens following TCR gene transfer: CD28-containing receptors mediate enhanced antigen-specific IFN{gamma} production Int. Immunol., April 1, 2006; 18(4): 591 - 601. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Price, J. M. Brenchley, L. E. Ruff, M. R. Betts, B. J. Hill, M. Roederer, R. A. Koup, S. A. Migueles, E. Gostick, L. Wooldridge, et al. Avidity for antigen shapes clonal dominance in CD8+ T cell populations specific for persistent DNA viruses J. Exp. Med., November 21, 2005; 202(10): 1349 - 1361. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Trautmann, M. Rimbert, K. Echasserieau, X. Saulquin, B. Neveu, J. Dechanet, V. Cerundolo, and M. Bonneville Selection of T Cell Clones Expressing High-Affinity Public TCRs within Human Cytomegalovirus-Specific CD8 T Cell Responses J. Immunol., November 1, 2005; 175(9): 6123 - 6132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Miles, D. Elhassen, N. A. Borg, S. L. Silins, F. E. Tynan, J. M. Burrows, A. W. Purcell, L. Kjer-Nielsen, J. Rossjohn, S. R. Burrows, et al. CTL Recognition of a Bulged Viral Peptide Involves Biased TCR Selection J. Immunol., September 15, 2005; 175(6): 3826 - 3834. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Salerno-Goncalves, R. Wahid, and M. B. Sztein Immunization of Volunteers with Salmonella enterica Serovar Typhi Strain Ty21a Elicits the Oligoclonal Expansion of CD8+ T Cells with Predominant V{beta} Repertoires Infect. Immun., June 1, 2005; 73(6): 3521 - 3530. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Facchinetti, S. D. Santa, S. Mezzalira, A. Rosato, and G. Biasi A Large Number of T Lymphocytes Recognize Moloney-Murine Leukemia Virus-Induced Antigens, but a Few Mediate Long-Lasting Tumor Immunosurveillance J. Immunol., May 1, 2005; 174(9): 5398 - 5406. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Curran, O. M. FitzGerald, P. J. Costello, J. M. Selby, D. J. Kane, B. Bresnihan, and R. Winchester Nucleotide Sequencing of Psoriatic Arthritis Tissue before and during Methotrexate Administration Reveals a Complex Inflammatory T Cell Infiltrate with Very Few Clones Exhibiting Features That Suggest They Drive the Inflammatory Process by Recognizing Autoantigens J. Immunol., February 1, 2004; 172(3): 1935 - 1944. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Hamrouni, A. Aublin, P. Guillaume, and J. L. Maryanski T Cell Receptor Gene Rearrangement Lineage Analysis Reveals Clues for the Origin of Highly Restricted Antigen-specific Repertoires J. Exp. Med., March 3, 2003; 197(5): 601 - 614. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Pilch, H. Hohn, C. Neukirch, K. Freitag, P. G. Knapstein, B. Tanner, and M. J. Maeurer Antigen-Driven T-Cell Selection in Patients with Cervical Cancer as Evidenced by T-Cell Receptor Analysis and Recognition of Autologous Tumor Clin. Vaccine Immunol., March 1, 2002; 9(2): 267 - 278. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Peggs, S. Verfuerth, A. Pizzey, J. Ainsworth, P. Moss, and S. Mackinnon Characterization of human cytomegalovirus peptide-specific CD8+ T-cell repertoire diversity following in vitro restimulation by antigen-pulsed dendritic cells Blood, January 1, 2002; 99(1): 213 - 223. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. D. Bourcier, D.-G. Lim, Y.-H. Ding, K. J. Smith, K. Wucherpfennig, and D. A. Hafler Conserved CDR3 Regions in T-Cell Receptor (TCR) CD8+ T Cells That Recognize the Tax11-19/HLA-A*0201 Complex in a Subject Infected with Human T-Cell Leukemia Virus Type 1: Relationship of T-Cell Fine Specificity and Major Histocompatibility Complex/Peptide/TCR Crystal Structure J. Virol., October 15, 2001; 75(20): 9836 - 9843. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. E. Annels, M. F. C. Callan, L. Tan, and A. B. Rickinson Changing Patterns of Dominant TCR Usage with Maturation of an EBV-Specific Cytotoxic T Cell Response J. Immunol., November 1, 2000; 165(9): 4831 - 4841. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
