The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lim, A.
Right arrow Articles by Bonneville, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lim, A.
Right arrow Articles by Bonneville, M.
The Journal of Immunology, 00, 165: 2001-2011.
Copyright © 00 by The American Association of Immunologists

Frequent Contribution of T Cell Clonotypes with Public TCR Features to the Chronic Response Against a Dominant EBV-Derived Epitope: Application to Direct Detection of Their Molecular Imprint on the Human Peripheral T Cell Repertoire1

Annick Lim*, Lydie Trautmann{dagger}, Marie-Alix Peyrat{dagger}, Chrystelle Couedel{dagger}, François Davodeau{dagger}, François Romagné{ddagger}, Philippe Kourilsky* and Marc Bonneville2,{dagger}

* Unité de Biologie Moléculaire du Gène, Institut National de la Santé et de la Recherche Médicale, Unité 277, Institut Pasteur, Paris, France; {dagger} Institut National de la Santé et de la Recherche Médicale, Unité 463, Institut de Biologie, Nantes, France; and {ddagger} Immunotech SA, Marseilles, France

In an attempt to provide a global picture of the TCR repertoire diversity of a chronic T cell response against a common Ag, we performed an extensive TCR analysis of cells reactive against a dominant HLA-A2-restricted EBV epitope (hereafter referred to as GLC/A2), obtained after sorting PBL or synovial fluid lymphocytes from EBV-seropositive individuals using MHC/peptide multimers. Although TCR ß-chain diversity of GLC/A2+ T cells was extensive and varied greatly from one donor to another, we identified in most cell lines several recurrent Vß subsets (Vß2, Vß4, and Vß16 positive) with highly conserved TCRß complementarity-determining region 3 (CDR3) length and junctional motifs, which represented from 11 to 98% (mean, 50%) of GLC/A2-reactive cells. While TCR ß-chains expressed by these subsets showed limited CDR1, CDR2, and CDR3 homology among themselves, their TCR {alpha}-chains comprised the same TCRAV region, thus suggesting hierarchical contribution of TCR {alpha}-chain vs TCR ß-chain CDR to recognition of this particular MHC/peptide complex. The common occurrence of T cell clonotypes with public TCR features within GLC/A2-specific T cells allowed their direct detection within unsorted PBL using ad hoc clonotypic primers. These results, which suggest an unexpectedly high contribution of public clonotypes to the TCR repertoire against a dominant epitope, have several implications for the follow-up and modulation of T cell-mediated immunity.




This article has been cited by other articles:


Home page
 J. Immunol.Home page
E. M. Iancu, P. Corthesy, P. Baumgaertner, E. Devevre, V. Voelter, P. Romero, D. E. Speiser, and N. Rufer
Clonotype Selection and Composition of Human CD8 T Cells Specific for Persistent Herpes Viruses Varies with Differentiation but Is Stable Over Time
J. Immunol., July 1, 2009; 183(1): 319 - 331.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
V. Venturi, H. Y. Chin, T. E. Asher, K. Ladell, P. Scheinberg, E. Bornstein, D. van Bockel, A. D. Kelleher, D. C. Douek, D. A. Price, et al.
TCR {beta}-Chain Sharing in Human CD8+ T Cell Responses to Cytomegalovirus and EBV
J. Immunol., December 1, 2008; 181(11): 7853 - 7862.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
V. Venturi, H. Y. Chin, D. A. Price, D. C. Douek, and M. P. Davenport
The Role of Production Frequency in the Sharing of Simian Immunodeficiency Virus-Specific CD8+ TCRs between Macaques
J. Immunol., August 15, 2008; 181(4): 2597 - 2609.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
V. Venturi, K. Kedzierska, D. A. Price, P. C. Doherty, D. C. Douek, S. J. Turner, and M. P. Davenport
Sharing of T cell receptors in antigen-specific responses is driven by convergent recombination
PNAS, December 5, 2006; 103(49): 18691 - 18696.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. E. Speiser, P. Baumgaertner, C. Barbey, V. Rubio-Godoy, A. Moulin, P. Corthesy, E. Devevre, P.-Y. Dietrich, D. Rimoldi, D. Lienard, et al.
A Novel Approach to Characterize Clonality and Differentiation of Human Melanoma-Specific T Cell Responses: Spontaneous Priming and Efficient Boosting by Vaccination
J. Immunol., July 15, 2006; 177(2): 1338 - 1348.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
N. Schaft, B. Lankiewicz, J. Drexhage, C. Berrevoets, D. J. Moss, V. Levitsky, M. Bonneville, S. P. Lee, A. J. McMichael, J.-W. Gratama, et al.
T cell re-targeting to EBV antigens following TCR gene transfer: CD28-containing receptors mediate enhanced antigen-specific IFN{gamma} production
Int. Immunol., April 1, 2006; 18(4): 591 - 601.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
D. A. Price, J. M. Brenchley, L. E. Ruff, M. R. Betts, B. J. Hill, M. Roederer, R. A. Koup, S. A. Migueles, E. Gostick, L. Wooldridge, et al.
Avidity for antigen shapes clonal dominance in CD8+ T cell populations specific for persistent DNA viruses
J. Exp. Med., November 21, 2005; 202(10): 1349 - 1361.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. Trautmann, M. Rimbert, K. Echasserieau, X. Saulquin, B. Neveu, J. Dechanet, V. Cerundolo, and M. Bonneville
Selection of T Cell Clones Expressing High-Affinity Public TCRs within Human Cytomegalovirus-Specific CD8 T Cell Responses
J. Immunol., November 1, 2005; 175(9): 6123 - 6132.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. J. Miles, D. Elhassen, N. A. Borg, S. L. Silins, F. E. Tynan, J. M. Burrows, A. W. Purcell, L. Kjer-Nielsen, J. Rossjohn, S. R. Burrows, et al.
CTL Recognition of a Bulged Viral Peptide Involves Biased TCR Selection
J. Immunol., September 15, 2005; 175(6): 3826 - 3834.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
R. Salerno-Goncalves, R. Wahid, and M. B. Sztein
Immunization of Volunteers with Salmonella enterica Serovar Typhi Strain Ty21a Elicits the Oligoclonal Expansion of CD8+ T Cells with Predominant V{beta} Repertoires
Infect. Immun., June 1, 2005; 73(6): 3521 - 3530.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Facchinetti, S. D. Santa, S. Mezzalira, A. Rosato, and G. Biasi
A Large Number of T Lymphocytes Recognize Moloney-Murine Leukemia Virus-Induced Antigens, but a Few Mediate Long-Lasting Tumor Immunosurveillance
J. Immunol., May 1, 2005; 174(9): 5398 - 5406.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. A. Curran, O. M. FitzGerald, P. J. Costello, J. M. Selby, D. J. Kane, B. Bresnihan, and R. Winchester
Nucleotide Sequencing of Psoriatic Arthritis Tissue before and during Methotrexate Administration Reveals a Complex Inflammatory T Cell Infiltrate with Very Few Clones Exhibiting Features That Suggest They Drive the Inflammatory Process by Recognizing Autoantigens
J. Immunol., February 1, 2004; 172(3): 1935 - 1944.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
A. Hamrouni, A. Aublin, P. Guillaume, and J. L. Maryanski
T Cell Receptor Gene Rearrangement Lineage Analysis Reveals Clues for the Origin of Highly Restricted Antigen-specific Repertoires
J. Exp. Med., March 3, 2003; 197(5): 601 - 614.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
H. Pilch, H. Hohn, C. Neukirch, K. Freitag, P. G. Knapstein, B. Tanner, and M. J. Maeurer
Antigen-Driven T-Cell Selection in Patients with Cervical Cancer as Evidenced by T-Cell Receptor Analysis and Recognition of Autologous Tumor
Clin. Vaccine Immunol., March 1, 2002; 9(2): 267 - 278.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
K. Peggs, S. Verfuerth, A. Pizzey, J. Ainsworth, P. Moss, and S. Mackinnon
Characterization of human cytomegalovirus peptide-specific CD8+ T-cell repertoire diversity following in vitro restimulation by antigen-pulsed dendritic cells
Blood, January 1, 2002; 99(1): 213 - 223.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
K. D. Bourcier, D.-G. Lim, Y.-H. Ding, K. J. Smith, K. Wucherpfennig, and D. A. Hafler
Conserved CDR3 Regions in T-Cell Receptor (TCR) CD8+ T Cells That Recognize the Tax11-19/HLA-A*0201 Complex in a Subject Infected with Human T-Cell Leukemia Virus Type 1: Relationship of T-Cell Fine Specificity and Major Histocompatibility Complex/Peptide/TCR Crystal Structure
J. Virol., October 15, 2001; 75(20): 9836 - 9843.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. E. Annels, M. F. C. Callan, L. Tan, and A. B. Rickinson
Changing Patterns of Dominant TCR Usage with Maturation of an EBV-Specific Cytotoxic T Cell Response
J. Immunol., November 1, 2000; 165(9): 4831 - 4841.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2000 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2000 by The American Association of Immunologists, Inc. All rights reserved.